cDNA Clone NOD2 (mouse)

• Plasmid: pCMV-FLAG-1 NOD2
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG Nod2
• Genbank: BC044774
• Tag: FLAG
• Species: Mouse

Sequence of Insert

GGATCCATGTGCTCACAGGAAGAGTTCCAGGCACAGAGGAGCCAGCTGGT
GGCACTGCTGATCTCAGGGTCCTTGGAGGGCTTTGAGAGCATCTTAGACT
GGCTGCTGTCTTGGGATGTGCTCTCCAGGGAGGACTATGAGGGCCTCAGC
CTCCCTGGCCAACCTCTCTCCCATTCTGCCAGGCGTCTGCTGGACACAGT
CTGGAACAAGGGTGTTTGGGGCTGTCAGAAGCTCCTCGAAGCTGTGCAGG
AGGCACAGGCCAACAGTCATACCTTTGAACTGTATGGGTCCTGGGACACT
CACTCCCTTCATCCAACCAGAGACCTGCAGAGTCACCGGCCAGCCATTGT
GAGGAGACTCTACAACCATGTAGAAGCCATGCTGGAGCTGGCAAGGGAGG
GGGGGTTTCTGAGCCAGTACGAGTGTGAGGAGATCAGGCTGCCAATCTTC
ACGTCGTCTCAGAGGGCAAGAAGGCTGCTTGATCTCGCTGCGGTGAAGGC
CAATGGACTGGCTGCCTTCCTTCTACAGCACGTCAGGGAACTGCCAGCTC
CACTGCCTCTGCCTTACGAGGGCTACTCTCTGTGTAGGTCCCGCTGTGAC
CGTGGCTTCACCCTCATTTGCCTCTTCTGTATCCTAGCTGCTGAGTGTCA
GAAGTTCATATCTAAGCTGAGGACCATGGTGTTGGCTCAGTCTCGCTTCC
TCAGTACTTACGATGGGTCAGAGAATCTTTGCCTGGAGGATATATACACG
GAGAACATCTTGGAGCTGCGGACTGAAGTGGGCACAGCCGGGGCCTTGCA
GAAGAGCCCTGCCATCCTGGGCCTGGAGGACCTCTTTGATACCCATGGTC
ACCTGAACAGAGATGCCGACACCATACTGGTGGTGGGCGAAGCAGGCAGT
GGCAAGAGCACTCTTCTGCAGCGTTTGCACCTGCTGTGGGCAACAGGGAG
GAGCTTCCAGGAGTTTCTCTTCATTTTCCCATTCAGCTGCCGACAGTTGC
AATGCGTGGCCAAACCGCTGTCCCTGAGGACGCTGCTCTTTGAGCACTGC
TGCTGGCCTGATGTCGCTCAGGACGATGTCTTCCAGTTCCTTCTTGACCA
TCCTGACCGTGTCCTGTTAACCTTTGATGGCTTGGACGAGTTCAAGTTCC
GGTTCACCGACCGGGAGCGCCACTGCTCTCCAATTGACCCCACGTCAGTC
CAGACTCTGCTCTTCAACCTTCTCCAGGGGAACCTGCTGAAGAATGCCTG
CAAGGTGCTGACCAGCCGTCCGGATGCTGTGTCAGCGCTCCTCAGGAAGT
TCGTCCGTACAGAGTGCCAACTGAAGGGCTTCTCTGAAGAGGGCATCCAA
CTGTACCTGAGAAAGCACCACCGGGAACCTGGGGTGGCAGACCGCCTCAT
CCAGCTGATCCAAGCCACCTCAGCCCTGCATGGTTTGTGCCACCTCCCTG
TCTTCTCTTGGATGGTGTCCAGATGCCACCGGGAACTTTTGCTGCAGAAC
AGGGTCTTCCCAACAACCAGCACGGACATGTACCTCCTGATCCTGCAGCA
TTTCCTGCTGCATGCCTCCCCTCCGGACTCCTCCCCACTCGGTCTGGGAC
CTGGACTCCTCCAAAGCCGGCTCTCCACCCTCCTGCACCTTGGCCACCTG
GCTCTCCGGGGCCTGGCCATGAGCTGCTATGTGTTCTCAGCCCAGCAGCT
CCAGGCAGCTCAGGTTGACTCTGATGATATTTCTCTTGGTTTCCTGGTAC
GTGCCCAAAGTAGTGTGCCCGGGAGCAAGGCGCCCCTGGAGTTCCTGCAC
ATTACCTTCCAGTGCTTTTTTGCCGCTTTCTACTTGGCTGTCAGTGCTGA
CACATCAGTGGCCTCTCTCAAGCACCTTTTCAGCTGTGGCCGGCTGGGCA
GCTCACTGCTGGGAAGGCTGCTGCCCAACCTGTGTATCCAGGGCTCCAGA
GTCAAGAAGGGCAGCGAAGCAGCCCTGCTGCAGAAGGCTGAGCCACACAA
CCTGCAGATCACAGCAGCCTTCCTAGCAGGTCTGTTGTCCCAGCAGCATC
GGGACCTGTTGGCTGCATGCCAGATCTCCGAGAGGGTGCTGCTCCAGCGT
CAGGCACGTGCCCGCTCGTGTCTGGCCCACAGCCTCCGCGAGCACTTCCA
TTCCATCCCGCCTGCCGTGCCCGGTGAGACCAAGAGCATGCATGCTATGC
CGGGCTTTATTTGGCTCATCCGGAGCCTGTACGAGATGCAGGAGGAGCAG
TTGGCCCAGGAGGCTGTCCGTCGCTTGGACATCGGGCACCTGAAGTTGAC
ATTTTGCAGAGTGGGCCCTGCAGAGTGTGCTGCGCTGGCCTTTGTACTGC
AACATCTCCAGCGGCCTGTGGCCCTACAGCTGGATTACAACTCTGTGGGA
GATGTCGGAGTGGAACAGCTGCGACCGTGCCTTGGGGTCTGCACAGCTCT
GTATTTGCGAGATAACAATATCTCAGACCGAGGTGCCCGCACGCTGGTTG
AGTGTGCTCTTCGCTGTGAGCAGCTGCAGAAACTAGCTCTCTTCAACAAC
AAACTCACGGATGCGTGCGCCTGCTCCATGGCCAAGCTCCTTGCACACAA
GCAGAACTTCTTGTCCCTGAGGGTGGGGAACAATCACATCACAGCCGCTG
GAGCCGAGGTGCTGGCCCAGGGACTCAAGAGCAACACCTCCCTGAAGTTC
CTGGGGTTCTGGGGCAACAGCGTGGGTGATAAGGGCACCCAAGCCCTGGC
TGAAGTTGTAGCCGACCACCAGAACCTAAAGTGGCTCAGCTTGGTAGGAA
ACAACATTGGCAGCATGGGTGCCCAAGCCCTAGCACTGATGCTGGAGAAG
AACAAGTCACTAGAGGAGCTCTGCCTAGAGGAAAACCATATCTGTGACGA
AGGGGTATATTCTCTCGCAGAAGGACTCAAGAGAAATTCAACTTTGAAAT
TCTTGAAACTGTCCAACAATGGCATCACCTACCGGGGTGCAGAAGCCCTC
TTGCAGGCCCTCAGCAGGAACAGTGCCATTCTGGAGGTTTGGCTTCGAGG
GAACACATTCTCTTTGGAGGAAATCCAAACACTGAGCTCCAGGGACGCCA
GACTCTTGTTGTGAGGATCC

Amino Acid Sequence

MDYKDDDDKGSMCSQEEFQAQRSQLVALLISGSLEGFESILDWLLSWDVL
SREDYEGLSLPGQPLSHSARRLLDTVWNKGVWGCQKLLEAVQEAQANSHT
FELYGSWDTHSLHPTRDLQSHRPAIVRRLYNHVEAMLELAREGGFLSQYE
CEEIRLPIFTSSQRARRLLDLAAVKANGLAAFLLQHVRELPAPLPLPYEG
YSLCRSRCDRGFTLICLFCILAAECQKFISKLRTMVLAQSRFLSTYDGSE
NLCLEDIYTENILELRTEVGTAGALQKSPAILGLEDLFDTHGHLNRDADT
ILVVGEAGSGKSTLLQRLHLLWATGRSFQEFLFIFPFSCRQLQCVAKPLS
LRTLLFEHCCWPDVAQDDVFQFLLDHPDRVLLTFDGLDEFKFRFTDRERH
CSPIDPTSVQTLLFNLLQGNLLKNACKVLTSRPDAVSALLRKFVRTECQL
KGFSEEGIQLYLRKHHREPGVADRLIQLIQATSALHGLCHLPVFSWMVSR
CHRELLLQNRVFPTTSTDMYLLILQHFLLHASPPDSSPLGLGPGLLQSRL
STLLHLGHLALRGLAMSCYVFSAQQLQAAQVDSDDISLGFLVRAQSSVPG
SKAPLEFLHITFQCFFAAFYLAVSADTSVASLKHLFSCGRLGSSLLGRLL
PNLCIQGSRVKKGSEAALLQKAEPHNLQITAAFLAGLLSQQHRDLLAACQ
ISERVLLQRQARARSCLAHSLREHFHSIPPAVPGETKSMHAMPGFIWLIR
SLYEMQEEQLAQEAVRRLDIGHLKLTFCRVGPAECAALAFVLQHLQRPVA
LQLDYNSVGDVGVEQLRPCLGVCTALYLRDNNISDRGARTLVECALRCEQ
LQKLALFNNKLTDACACSMAKLLAHKQNFLSLRVGNNHITAAGAEVLAQG
LKSNTSLKFLGFWGNSVGDKGTQALAEVVADHQNLKWLSLVGNNIGSMGA
QALALMLEKNKSLEELCLEENHICDEGVYSLAEGLKRNSTLKFLKLSNNG
ITYRGAEALLQALSRNSAILEVWLRGNTFSLEEIQTLSSRDARLLL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12976
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal FLAG for mammalian expression. Expressed protein has A628V change compare to above genbank. This agrees with genomic sequence. ; ; Estimated MW of expressed protein : 116609 Daltons