cDNA Clone USP20

• Plasmid: pET156P USP20
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His USP20
• Genbank: AAH39593.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGGGGACTCCAGGGACCTTTGCCCTCACCTTGACTCCATAGG
AGAGGTGACCAAAGAGGACTTGCTGCTCAAATCTAAGGGAACCTGTCAGT
CGTGTGGGGTCACCGGACCAAACCTATGGGCCTGTCTGCAGGTTGCCTGC
CCCTATGTTGGCTGCGGAGAATCCTTCGCTGACCACAGCACCATTCATGC
ACAGGCAAAAAAGCACAACTTGACCGTGAACCTGACCACGTTCCGACTGT
GGTGTTACGCCTGTGAGAAGGAGGTATTCCTGGAGCAGCGGCTGGCAGCC
CCTCTGCTGGGCTCCTCTTCCAAGTTCTCTGAACAGGACTCCCCGCCACC
CTCCCACCCTCTGAAAGCTGTTCCTATTGCTGTGGCTGATGAAGGAGAGT
CTGAGTCAGAGGATGATGACCTGAAACCTCGAGGCCTCACGGGCATGAAG
AACCTCGGGAACTCCTGCTACATGAACGCCGCCCTGCAGGCCCTGTCCAA
TTGCCCGCCGCTGACTCAGTTCTTCTTGGAGTGTGGCGGCCTGGTGCGCA
CAGATAAGAAGCCAGCCCTGTGCAAGAGCTACCAGAAGCTGGTCTCTGAG
GTCTGGCATAAGAAACGGCCAAGCTACGTGGTCCCCACCAGTCTGTCTCA
TGGGATCAAGTTGGTCAACCCAATGTTCCGAGGCTATGCCCAGCAGGACA
CCCAAGAGTTCCTTCGCTGCCTGATGGACCAGCTGCACGAGGAGCTCAAG
GAGCCGGTGGTGGCCACGGTGGCGCTGACGGAGGCTCGGGACTCAGATTC
GAGTGACACGGATGAGAAACGGGAGGGTGACCGGAGCCCATCAGAAGATG
AGTTCTTGTCCTGTGACTCGAGCAGTGACCGGGGTGAGGGTGACGGGCAG
GGGCGTGGCGGGGGCAGCTCGCAGGCCGAGACGGAGCTGCTGATCCCAGA
TGAGGCGGGCCGAGCCATCTCTGAGAAGGAGCGGATGAAGGACCGCAAGT
TCTCCTGGGGCCAGCAGCGTACAAACTCGGAGCAAGTGGACGAGGACGCT
GATGTGGACACTGCCATGGCTGCCCTTGACCAGCCCGCGGAGGCCCAGCC
CCCGTCACCACGGTCCTCCAGCCCCTGCCGGACGCCAGAGCCGGACAATG
ATGCTCACCTACGCAGCTCCTCTCGCCCCTGCAGCCCCGTCCACCACCAC
GAGGGCCATGCCAAGCTGTCTAGCAGCCCCCCTCGTGCAAGCCCCGTGAG
GATGGCACCGTCGTACGTGCTCAAGAAAGCCCAGGTATTGAGTGCTGGCA
GCCGGAGGCGGAAGGAGCAGCGCTACCGCAGCGTCATCTCAGACATCTTT
GACGGCTCCATTCTCAGCCTCGTGCAGTGTCTCACCTGTGACCGGGTATC
CACCACAGTGGAAACGTTCCAGGACTTATCACTGCCCATTCCTGGAAAGG
AGGACCTGGCCAAGCTCCATTCAGCCATCTACCAGAATGTGCCGGCCAAG
CCAGGCGCCTGTGGGGACAGCTATGCCGCCCAGGGCTGGCTGGCCTTCAT
TGTGGAGTACATCCGACGGTTTGTGGTATCCTGTACCCCCAGCTGGTTTT
GGGGGCCTGTCGTCACCCTGGAAGACTGCCTTGCTGCCTTCTTTGCCGCT
GATGAGTTAAAGGGTGACAACATGTACAGCTGTGAGCGGTGTAAGAAGCT
GCGGAACGGAGTGAAGTACTGCAAAGTCCTGCGGTTGCCCGAGATCCTGT
GCATTCACCTAAAGCGCTTTCGGCACGAGGTGATGTACTCATTCAAGATC
AACAGCCACGTCTCCTTCCCCCTCGAGGGGCTCGACCTGCGCCCCTTCCT
TGCCAAGGAGTGCACATCCCAGATCACCACCTACGACCTCCTCTCGGTCA
TCTGCCACCACGGCACGGCAGGCAGTGGGCACTACATCGCCTACTGCCAG
AACGTGATCAATGGGCAGTGGTACGAGTTTGATGACCAGTACGTCACAGA
AGTCCACGAGACGGTGGTGCAGAACGCCGAGGGCTACGTACTCTTCTACA
GGAAGAGCAGCGAGGAGGCCATGCGGGAGCGACAGCAGGTGGTGTCCCTG
GCCGCCATGCGGGAGCCCAGCCTGCTGCGGTTCTACGTGTCCCGCGAGTG
GCTCAACAAGTTCAACACCTTCGCGGAGCCAGGCCCCATCACCAACCAGA
CCTTCCTCTGCTCCCACGGAGGCATCCCGCCCCACAAATACCACTACATC
GACGACCTGGTGGTCATCCTGCCCCAGAACGTCTGGGAGCACCTGTACAA
CAGATTCGGGGGTGGCCCCGCCGTGAACCACCTGTACGTGTGCTCCATCT
GCCAGGTGGAGATCGAGGCACTGGCCAAGCGCAGGAGGATCGAGATCGAC
ACCTTCATCAAGTTGAACAAGGCCTTCCAGGCCGAGGAGTCGCCGGGCGT
CATCTACTGCATCAGCATGCAGTGGTTCCGGGAGTGGGAGGCGTTCGTCA
AGGGGAAGGACAACGAGCCCCCCGGGCCCATTGACAACAGCAGGATTGCA
CAGGTCAAAGGAAGCGGCCATGTCCAGCTGAAGCAGGGAGCTGACTACGG
GCAGATTTCGGAGGAGACCTGGACCTACCTGAACAGCCTGTATGGAGGTG
GCCCCGAGATTGCCATCCGCCAGAGTGTGGCGCAGCCGCTGGGCCCAGAG
AACCTGCACGGGGAGCAGAAGATCGAAGCCGAGACGCGGGCCGTGTGAGC
GGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMGDSRDLCPHLDSIGEVTKEDLLLKSK
GTCQSCGVTGPNLWACLQVACPYVGCGESFADHSTIHAQAKKHNLTVNLT
TFRLWCYACEKEVFLEQRLAAPLLGSSSKFSEQDSPPPSHPLKAVPIAVA
DEGESESEDDDLKPRGLTGMKNLGNSCYMNAALQALSNCPPLTQFFLECG
GLVRTDKKPALCKSYQKLVSEVWHKKRPSYVVPTSLSHGIKLVNPMFRGY
AQQDTQEFLRCLMDQLHEELKEPVVATVALTEARDSDSSDTDEKREGDRS
PSEDEFLSCDSSSDRGEGDGQGRGGGSSQAETELLIPDEAGRAISEKERM
KDRKFSWGQQRTNSEQVDEDADVDTAMAALDQPAEAQPPSPRSSSPCRTP
EPDNDAHLRSSSRPCSPVHHHEGHAKLSSSPPRASPVRMAPSYVLKKAQV
LSAGSRRRKEQRYRSVISDIFDGSILSLVQCLTCDRVSTTVETFQDLSLP
IPGKEDLAKLHSAIYQNVPAKPGACGDSYAAQGWLAFIVEYIRRFVVSCT
PSWFWGPVVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLRL
PEILCIHLKRFRHEVMYSFKINSHVSFPLEGLDLRPFLAKECTSQITTYD
LLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNAEGY
VLFYRKSSEEAMRERQQVVSLAAMREPSLLRFYVSREWLNKFNTFAEPGP
ITNQTFLCSHGGIPPHKYHYIDDLVVILPQNVWEHLYNRFGGGPAVNHLY
VCSICQVEIEALAKRRRIEIDTFIKLNKAFQAEESPGVIYCISMQWFREW
EAFVKGKDNEPPGPIDNSRIAQVKGSGHVQLKQGADYGQISEETWTYLNS
LYGGGPEIAIRQSVAQPLGPENLHGEQKIEAETRAV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12908
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression. ; ; Estimated MW of expressed protein : 104329 Daltons