cDNA Clone Nemo

• Plasmid: pcDNA5-FRT/TO Nemo FLAG
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: Nemo FLAG
• Genbank: AAH50612
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGCCACCATGAATAGGCACCTCTGGAAGAGCCAACTGTGTGAGAT
GGTGCAGCCCAGTGGTGGCCCGGCAGCAGATCAGGACGTACTGGGCGAAG
AGTCTCCTCTGGGGAAGCCAGCCATGCTGCACCTGCCTTCAGAACAGGGC
GCTCCTGAGACCCTCCAGCGCTGCCTGGAGGAGAATCAAGAGCTCCGAGA
TGCCATCCGGCAGAGCAACCAGATTCTGCGGGAGCGCTGCGAGGAGCTTC
TGCATTTCCAAGCCAGCCAGAGGGAGGAGAAGGAGTTCCTCATGTGCAAG
TTCCAGGAGGCCAGGAAACTGGTGGAGAGACTCGGCCTGGAGAAGCTCGA
TCTGAAGAGGCAGAAGGAGCAGGCTCTGCGGGAGGTGGAGCACCTGAAGA
GATGCCAGCAGCAGATGGCTGAGGACAAGGCCTCTGTGAAAGCCCAGGTG
ACGTCCTTGCTCGGGGAGCTGCAGGAGAGCCAGAGTCGCTTGGAGGCTGC
CACTAAGGAATGCCAGGCTCTGGAGGGTCGGGCCCGGGCGGCCAGCGAGC
AGGCGCGGCAGCTGGAGAGTGAGCGCGAGGCGCTGCAGCAGCAGCACAGC
GTGCAGGTGGACCAGCTGCGCATGCAGGGCCAGAGCGTGGAGGCCGCGCT
CCGCATGGAGCGCCAGGCCGCCTCGGAGGAGAAGAGGAAGCTGGCCCAGT
TGCAGGTGGCCTATCACCAGCTCTTCCAAGAATACGACAACCACATCAAG
AGCAGCGTGGTGGGCAGTGAGCGGAAGCGAGGAATGCAGCTGGAAGATCT
CAAACAGCAGCTCCAGCAGGCCGAGGAGGCCCTGGTGGCCAAACAGGAGG
TGATCGATAAGCTGAAGGAGGAGGCCGAGCAGCACAAGATTGTGATGGAG
ACCGTTCCGGTGCTGAAGGCCCAGGCGGATATCTACAAGGCGGACTTCCA
GGCTGAGAGGCAGGCCCGGGAGAAGCTGGCCGAGAAGAAGGAGCTCCTGC
AGGAGCAGCTGGAGCAGCTGCAGAGGGAGTACAGCAAACTGAAGGCCAGC
TGTCAGGAGTCGGCCAGGATCGAGGACATGAGGAAGCGGCATGTCGAGGT
CTCCCAGGCCCCCTTGCCCCCCGCCCCTGCCTACCTCTCCTCTCCCCTGG
CCCTGCCCAGCCAGAGGAGGAGCCCCCCCGAGGAGCCACCTGACTTCTGC
TGTCCCAAGTGCCAGTATCAGGCCCCTGATATGGACACCCTGCAGATACA
TGTCATGGAGTGCATTGAGGACTACAAGGACGATGACGATAAGTAAGCGG
CCGC

Amino Acid Sequence

MNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPET
LQRCLEENQELRDAIRQSNQILRERCEELLHFQASQREEKEFLMCKFQEA
RKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQMAEDKASVKAQVTSLL
GELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVD
QLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVV
GSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPV
LKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYSKLKASCQES
ARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKC
QYQAPDMDTLQIHVMECIEDYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12840
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C ter FLAG for mammalian expression.; ; Estimated MW of expressed protein : 49190 Daltons