cDNA Clone USP20

• Synonyms: KIAA1003, LSFR3A, VDU2
• Plasmid: pCMV-FLAG-1 USP20
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG USP20
• Genbank: AAH39593.1
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGGGGACTCCAGGGACCTTTGCCCTCACCTTGACTCCATAGG
AGAGGTGACCAAAGAGGACTTGCTGCTCAAATCTAAGGGAACCTGTCAGT
CGTGTGGGGTCACCGGACCAAACCTATGGGCCTGTCTGCAGGTTGCCTGC
CCCTATGTTGGCTGCGGAGAATCCTTCGCTGACCACAGCACCATTCATGC
ACAGGCAAAAAAGCACAACTTGACCGTGAACCTGACCACGTTCCGACTGT
GGTGTTACGCCTGTGAGAAGGAGGTATTCCTGGAGCAGCGGCTGGCAGCC
CCTCTGCTGGGCTCCTCTTCCAAGTTCTCTGAACAGGACTCCCCGCCACC
CTCCCACCCTCTGAAAGCTGTTCCTATTGCTGTGGCTGATGAAGGAGAGT
CTGAGTCAGAGGATGATGACCTGAAACCTCGAGGCCTCACGGGCATGAAG
AACCTCGGGAACTCCTGCTACATGAACGCCGCCCTGCAGGCCCTGTCCAA
TTGCCCGCCGCTGACTCAGTTCTTCTTGGAGTGTGGCGGCCTGGTGCGCA
CAGATAAGAAGCCAGCCCTGTGCAAGAGCTACCAGAAGCTGGTCTCTGAG
GTCTGGCATAAGAAACGGCCAAGCTACGTGGTCCCCACCAGTCTGTCTCA
TGGGATCAAGTTGGTCAACCCAATGTTCCGAGGCTATGCCCAGCAGGACA
CCCAAGAGTTCCTTCGCTGCCTGATGGACCAGCTGCACGAGGAGCTCAAG
GAGCCGGTGGTGGCCACGGTGGCGCTGACGGAGGCTCGGGACTCAGATTC
GAGTGACACGGATGAGAAACGGGAGGGTGACCGGAGCCCATCAGAAGATG
AGTTCTTGTCCTGTGACTCGAGCAGTGACCGGGGTGAGGGTGACGGGCAG
GGGCGTGGCGGGGGCAGCTCGCAGGCCGAGACGGAGCTGCTGATCCCAGA
TGAGGCGGGCCGAGCCATCTCTGAGAAGGAGCGGATGAAGGACCGCAAGT
TCTCCTGGGGCCAGCAGCGTACAAACTCGGAGCAAGTGGACGAGGACGCT
GATGTGGACACTGCCATGGCTGCCCTTGACCAGCCCGCGGAGGCCCAGCC
CCCGTCACCACGGTCCTCCAGCCCCTGCCGGACGCCAGAGCCGGACAATG
ATGCTCACCTACGCAGCTCCTCTCGCCCCTGCAGCCCCGTCCACCACCAC
GAGGGCCATGCCAAGCTGTCTAGCAGCCCCCCTCGTGCAAGCCCCGTGAG
GATGGCACCGTCGTACGTGCTCAAGAAAGCCCAGGTATTGAGTGCTGGCA
GCCGGAGGCGGAAGGAGCAGCGCTACCGCAGCGTCATCTCAGACATCTTT
GACGGCTCCATTCTCAGCCTCGTGCAGTGTCTCACCTGTGACCGGGTATC
CACCACAGTGGAAACGTTCCAGGACTTATCACTGCCCATTCCTGGAAAGG
AGGACCTGGCCAAGCTCCATTCAGCCATCTACCAGAATGTGCCGGCCAAG
CCAGGCGCCTGTGGGGACAGCTATGCCGCCCAGGGCTGGCTGGCCTTCAT
TGTGGAGTACATCCGACGGTTTGTGGTATCCTGTACCCCCAGCTGGTTTT
GGGGGCCTGTCGTCACCCTGGAAGACTGCCTTGCTGCCTTCTTTGCCGCT
GATGAGTTAAAGGGTGACAACATGTACAGCTGTGAGCGGTGTAAGAAGCT
GCGGAACGGAGTGAAGTACTGCAAAGTCCTGCGGTTGCCCGAGATCCTGT
GCATTCACCTAAAGCGCTTTCGGCACGAGGTGATGTACTCATTCAAGATC
AACAGCCACGTCTCCTTCCCCCTCGAGGGGCTCGACCTGCGCCCCTTCCT
TGCCAAGGAGTGCACATCCCAGATCACCACCTACGACCTCCTCTCGGTCA
TCTGCCACCACGGCACGGCAGGCAGTGGGCACTACATCGCCTACTGCCAG
AACGTGATCAATGGGCAGTGGTACGAGTTTGATGACCAGTACGTCACAGA
AGTCCACGAGACGGTGGTGCAGAACGCCGAGGGCTACGTACTCTTCTACA
GGAAGAGCAGCGAGGAGGCCATGCGGGAGCGACAGCAGGTGGTGTCCCTG
GCCGCCATGCGGGAGCCCAGCCTGCTGCGGTTCTACGTGTCCCGCGAGTG
GCTCAACAAGTTCAACACCTTCGCGGAGCCAGGCCCCATCACCAACCAGA
CCTTCCTCTGCTCCCACGGAGGCATCCCGCCCCACAAATACCACTACATC
GACGACCTGGTGGTCATCCTGCCCCAGAACGTCTGGGAGCACCTGTACAA
CAGATTCGGGGGTGGCCCCGCCGTGAACCACCTGTACGTGTGCTCCATCT
GCCAGGTGGAGATCGAGGCACTGGCCAAGCGCAGGAGGATCGAGATCGAC
ACCTTCATCAAGTTGAACAAGGCCTTCCAGGCCGAGGAGTCGCCGGGCGT
CATCTACTGCATCAGCATGCAGTGGTTCCGGGAGTGGGAGGCGTTCGTCA
AGGGGAAGGACAACGAGCCCCCCGGGCCCATTGACAACAGCAGGATTGCA
CAGGTCAAAGGAAGCGGCCATGTCCAGCTGAAGCAGGGAGCTGACTACGG
GCAGATTTCGGAGGAGACCTGGACCTACCTGAACAGCCTGTATGGAGGTG
GCCCCGAGATTGCCATCCGCCAGAGTGTGGCGCAGCCGCTGGGCCCAGAG
AACCTGCACGGGGAGCAGAAGATCGAAGCCGAGACGCGGGCCGTGTGAGC
GGCCGC

Amino Acid Sequence

MDYKDDDDKGSMGDSRDLCPHLDSIGEVTKEDLLLKSKGTCQSCGVTGPN
LWACLQVACPYVGCGESFADHSTIHAQAKKHNLTVNLTTFRLWCYACEKE
VFLEQRLAAPLLGSSSKFSEQDSPPPSHPLKAVPIAVADEGESESEDDDL
KPRGLTGMKNLGNSCYMNAALQALSNCPPLTQFFLECGGLVRTDKKPALC
KSYQKLVSEVWHKKRPSYVVPTSLSHGIKLVNPMFRGYAQQDTQEFLRCL
MDQLHEELKEPVVATVALTEARDSDSSDTDEKREGDRSPSEDEFLSCDSS
SDRGEGDGQGRGGGSSQAETELLIPDEAGRAISEKERMKDRKFSWGQQRT
NSEQVDEDADVDTAMAALDQPAEAQPPSPRSSSPCRTPEPDNDAHLRSSS
RPCSPVHHHEGHAKLSSSPPRASPVRMAPSYVLKKAQVLSAGSRRRKEQR
YRSVISDIFDGSILSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHS
AIYQNVPAKPGACGDSYAAQGWLAFIVEYIRRFVVSCTPSWFWGPVVTLE
DCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLRLPEILCIHLKRFR
HEVMYSFKINSHVSFPLEGLDLRPFLAKECTSQITTYDLLSVICHHGTAG
SGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNAEGYVLFYRKSSEEAM
RERQQVVSLAAMREPSLLRFYVSREWLNKFNTFAEPGPITNQTFLCSHGG
IPPHKYHYIDDLVVILPQNVWEHLYNRFGGGPAVNHLYVCSICQVEIEAL
AKRRRIEIDTFIKLNKAFQAEESPGVIYCISMQWFREWEAFVKGKDNEPP
GPIDNSRIAQVKGSGHVQLKQGADYGQISEETWTYLNSLYGGGPEIAIRQ
SVAQPLGPENLHGEQKIEAETRAV

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV-FLAG-1 USP20441.03 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU12778
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression. ; ; Estimated MW of expressed protein : 103154 Daltons
• Low Temp: No