cDNA Clone VPS15

• Plasmid: pEBG6P-VPS15
• Parent Plasmid: pEBG6P
• Expressed: GST-VPS15
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGGAAATCAGCTTGCTGGCATTGCTCCCTCCCAGATCCTTTCTGTAGAGAGTTATTTTTCAGATATTCATGACTTTGAATATGATAAAA
GCCTGGGGAGTACTCGGTTTTTTAAAGTTGCTCGAGCCAAGCACCGAGAAGGCCTGGTCGTTGTGAAGGTTTTTGCAATTCAGGATCCCACATTGCCTTT
AACCAGCTATAAACAAGAGCTGGAGGAACTGAAAATCAGGCTTAATTCTGCACAGAATTGTCTACCTTTCCAGAAAGCATCAGAAAAAGCATCTGAGAAA
GCAGCTATGCTCTTTAGGCAGTATGTGCGAGACAATCTCTATGATCGCATCAGTACCCGTCCATTCTTGAATAACATTGAGAAGCGCTGGATTGCTTTCC
AGATCCTGACAGCTGTGGACCAAGCACACAAATCTGGAGTTCGTCATGGGGACATCAAGACTGAGAATGTGATGGTCACCAGTTGGAATTGGGTTCTTCT
AACTGATTTTGCCAGTTTTAAGCCCACTTATCTTCCAGAAGACAACCCGGCAGATTTCAATTATTTCTTTGACACATCACGGAGGAGAACTTGCTATATT
GCTCCTGAACGTTTTGTTGATGGTGGGATGTTTGCCACTGAGTTAGAATATATGAGAGATCCTTCAACTCCGCTTGTAGACTTAAATAGCAATCAGAGAA
CAAGAGGAGAGTTGAAGAGAGCAATGGACATCTTTTCAGCAGGTTGTGTGATAGCTGAGCTTTTTACAGAAGGTGTACCATTATTTGATCTCTCTCAACT
TTTGGCTTATAGAAATGGACATTTTTTCCCTGAACAAGTGCTAAATAAAATTGAAGATCACAGTATCAGAGAATTGGTAACTCAGATGATTCACCGTGAG
CCAGATAAACGTTTAGAGGCAGAAGATTACTTAAAACAGCAGCGTGGCAATGCCTTTCCTGAAATATTTTACACTTTTCTTCAGCCCTACATGGCCCAGT
TTGCCAAGGAAACGTTTCTTTCTGCAGATGAGCGTATTCTGGTTATACGGAAGGATTTGGGCAACATTATTCACAATCTCTGTGGACATGATCTGCCAGA
AAAAGCCGAAGGAGAGCCTAAGGAAAATGGGCTGGTTATCTTGGTATCTGTTATAACATCCTGCCTACAGACCCTTAAATACTGTGATTCCAAACTAGCT
GCTTTGGAACTGATTCTTCATTTGGCTCCAAGATTAAGTGTTGAAATCCTTTTGGATCGTATTACTCCATATCTTTTGCATTTCAGCAATGACTCTGTTC
CTAGGGTGAGGGCTGAAGCCTTGAGGACGTTGACCAAAGTTCTTGCTCTCGTCAAAGAGGTTCCTCGTAATGATATCAATATTTATCCGGAATACATTCT
GCCAGGCATAGCCCACTTAGCCCAAGATGATGCTACTATCGTTAGACTAGCCTATGCTGAAAACATAGCTCTGCTGGCAGAAACAGCTCTGAGATTCCTG
GAATTAGTACAGTTAAAAAATCTTAATATGGAAAATGACCCCAATAATGAAGAAATAGATGAGGTTACACATCCAAATGGAAATTATGACACAGAGCTCC
AAGCCTTACATGAAATGGTCCAGCAGAAAGTTGTTACTTTGCTAAGTGACCCTGAAAATATTGTAAAACAAACCTTGATGGAAAATGGAATAACACGGCT
GTGTGTATTCTTTGGACGTCAGAAAGCCAACGATGTTTTGTTGTCCCACATGATTACTTTCCTAAATGATAAGAATGATTGGCATCTACGTGGAGCATTT
TTTGATAGTATAGTTGGTGTTGCTGCCTATGTTGGCTGGCAAAGCTCCTCAATTCTCAAGCCTCTGCTGCAACAAGGTCTTAGTGATGCTGAGGAATTTG
TCATTGTGAAAGCTCTTTATGCCCTTACTTGTATGTGCCAGTTAGGACTGCTACAAAAACCCCATGTTTACGAATTTGCCAGTGATATTGCCCCCTTCCT
GTGTCATCCCAATTTATGGATACGTTATGGTGCCGTGGGATTTATCACAGTGGTAGCTCGTCAAATAAGTACAGCTGATGTCTACTGTAAACTGATGCCT
TATCTTGACCCATATATTACCCAACCAATAATACAGATTGAAAGAAAACTTGTTCTGCTCAGTGTTTTAAAGGAACCAGTAAGTCGTTCTATATTTGATT
ATGCTTTGAGGTCTAAAGATATTACTAGCTTGTTCAGACATCTTCACATGCGTCAGAAGAAACGAAATGGTTCTCTTCCCGACTGCCCTCCGCCAGAGGA
TCCTGCCATAGCACAGCTTCTGAAGAAGTTGCTCTCACAGGGAATGACAGAGGAAGAGGAAGACAAACTTCTGGCACTGAAAGACTTCATGATGAAATCT
AATAAAGCAAAGGCCAATATAGTGGACCAGAGCCATCTTCATGATAGTAGTCAGAAAGGTGTAATTGACTTGGCAGCTTTAGGCATAACTGGGAGACAAG
TTGATCTTGTTAAAACCAAACAAGAACCAGATGACAAACGGGCCAGAAAACATGTAAAACAAGACTCAAATGTAAATGAAGAATGGAAAAGCATGTTTGG
GTCACTGGACCCACCAAACATGCCACAGGCCCTACCTAAAGGGAGTGATCAGGAGGTGATTCAGACTGGGAAACCTCCTCGTTCCGAGTCCTCTGCTGGC
ATTTGTGTCCCTTTGTCAACTTCTTCACAGGTTCCAGAAGTGACAACTGTCCAAAATAAAAAACCAGTAATACCGGTTTTAAGTAGTACAATCTTACCAT
CCACCTATCAGATTCGAATTACAACTTGTAAAACTGAACTTCAGCAACTCATCCAGCAAAAGCGGGAGCAGTGCAATGCTGAGAGAATAGCTAAGCAGAT
GATGGAAAATGCTGAATGGGAGAGTAAACCACCACCACCTGGATGGCGTCCTAAAGGGCTGTTAGTTGCCCATCTTCATGAGCATAAATCTGCTGTGAAT
CGAATTAGAGTCTCTGATGAACACTCACTTTTTGCAACATGTTCAAATGATGGCACAGTGAAAATCTGGAACAGTCAAAAGATGGAGGGGAAGACCACCA
CTACCAGATCTATTCTTACATACAGCCGAATTGGAGGACGAGTCAAGACGCTCACATTCTGCCAAGGCTCCCACTATTTAGCCATAGCATCTGATAATGG
TGCTGTCCAGCTTCTTGGAATTGAGGCTTCTAAGCTGCCCAAGTCTCCTAAAATCCATCCTCTACAAAGCAGAATTCTAGATCAGAAGGAGGACGGTTGT
GTTGTGGATATGCATCACTTCAACTCTGGAGCACAGTCTGTTCTTGCCTATGCCACTGTGAATGGCTCTCTGGTTGGCTGGGACCTTAGGTCTTCAAGCA
ATGCGTGGACTTTAAAGCATGATTTAAAGTCGGGCCTCATCACTTCCTTTGCTGTGGACATCCACCAATGCTGGCTCTGCATTGGTACAAGCAGTGGTAC
CATGGCTTGTTGGGACATGAGGTTCCAGTTGCCAATTTCAAGTCACTGTCATCCTTCCAGGGCTCGAATCAGACGCCTCTCAATGCACCCTCTGTATCAG
TCCTGGGTGATTGCAGCTGTTCAGGGCAACAACGAAGTGTCCATGTGGGACATGGAGACTGGTGACAGAAGATTTACTCTCTGGGCCAGCAGTGCACCAC
CACTTTCTGAATTACAGCCTTCTCCTCATAGCGTCCATGGTATCTACTGTAGTCCTGCAGATGGAAATCCTATCCTACTAACAGCTGGCTCAGATATGAA
AATAAGGTTTTGGGACTTGGCTTACCCAGAAAGGTCCTATGTTGTTGCAGGAAGTACTAGTTCCCCATCTGTGTCCTACTACAGGAAAATAATTGAAGGC
ACTGAAGTTGTCCAGGAAATTCAGAATAAGCAGAAAGTAGGACCAAGTGATGACACCCCTCGAAGGGGCCCAGAGTCCCTGCCCGTGGGACATCATGACA
TCATCACTGATGTCGCCACATTCCAGACCACACAGGGCTTCATCGTAACTGCTTCTAGAGATGGGATTGTGAAGGTGTGGAAATAAGTCGACAAGGGCGA
ATTCCACATTGGTCGCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMGNQLAGIAPSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAKHREGLVVVKV
FAIQDPTLPLTSYKQELEELKIRLNSAQNCLPFQKASEKASEKAAMLFRQYVRDNLYDRISTRPFLNNIEKRWIAFQILTAVDQAHKSGVRHGDIKTENV
MVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGGMFATELEYMRDPSTPLVDLNSNQRTRGELKRAMDIFSAGCVIAELFTE
GVPLFDLSQLLAYRNGHFFPEQVLNKIEDHSIRELVTQMIHREPDKRLEAEDYLKQQRGNAFPEIFYTFLQPYMAQFAKETFLSADERILVIRKDLGNII
HNLCGHDLPEKAEGEPKENGLVILVSVITSCLQTLKYCDSKLAALELILHLAPRLSVEILLDRITPYLLHFSNDSVPRVRAEALRTLTKVLALVKEVPRN
DINIYPEYILPGIAHLAQDDATIVRLAYAENIALLAETALRFLELVQLKNLNMENDPNNEEIDEVTHPNGNYDTELQALHEMVQQKVVTLLSDPENIVKQ
TLMENGITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAAYVGWQSSSILKPLLQQGLSDAEEFVIVKALYALTCMCQLGLLQKPHVY
EFASDIAPFLCHPNLWIRYGAVGFITVVARQISTADVYCKLMPYLDPYITQPIIQIERKLVLLSVLKEPVSRSIFDYALRSKDITSLFRHLHMRQKKRNG
SLPDCPPPEDPAIAQLLKKLLSQGMTEEEEDKLLALKDFMMKSNKAKANIVDQSHLHDSSQKGVIDLAALGITGRQVDLVKTKQEPDDKRARKHVKQDSN
VNEEWKSMFGSLDPPNMPQALPKGSDQEVIQTGKPPRSESSAGICVPLSTSSQVPEVTTVQNKKPVIPVLSSTILPSTYQIRITTCKTELQQLIQQKREQ
CNAERIAKQMMENAEWESKPPPPGWRPKGLLVAHLHEHKSAVNRIRVSDEHSLFATCSNDGTVKIWNSQKMEGKTTTTRSILTYSRIGGRVKTLTFCQGS
HYLAIASDNGAVQLLGIEASKLPKSPKIHPLQSRILDQKEDGCVVDMHHFNSGAQSVLAYATVNGSLVGWDLRSSSNAWTLKHDLKSGLITSFAVDIHQC
WLCIGTSSGTMACWDMRFQLPISSHCHPSRARIRRLSMHPLYQSWVIAAVQGNNEVSMWDMETGDRRFTLWASSAPPLSELQPSPHSVHGIYCSPADGNP
ILLTAGSDMKIRFWDLAYPERSYVVAGSTSSPSVSYYRKIIEGTEVVQEIQNKQKVGPSDDTPRRGPESLPVGHHDIITDVATFQTTQGFIVTASRDGIV
KVWK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12737
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No