cDNA Clone SMAD7

• Plasmid: pEBG6P-SMAD7
• Parent Plasmid: pEBG6P
• Expressed: GST-SMAD7
• Genbank: O15105
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTTCAGGACCAAACGATCTGCGCTCGTCCGGCGTCTCTGGAG
GAGCCGTGCGCCCGGCGGCGAGGACGAGGAGGAGGGCGCAGGGGGAGGTG
GAGGAGGAGGCGAGCTGCGGGGAGAAGGGGCGACGGACAGCCGAGCGCAT
GGGGCCGGTGGCGGCGGCCCGGGCAGGGCTGGATGCTGCCTGGGCAAGGC
GGTGCGAGGTGCCAAAGGTCACCACCATCCCCACCCGCCAGCCGCGGGCG
CCGGCGCGGCCGGGGGCGCCGAGGCGGATCTGAAGGCGCTCACGCACTCG
GTGCTCAAGAAACTGAAGGAGCGGCAGCTGGAGCTGCTGCTCCAGGCCGT
GGAGTCCCGCGGCGGGACGCGCACCGCGTGCCTCCTGCTGCCCGGCCGCC
TGGACTGCAGGCTGGGCCCGGGGGCGCCCGCCGGCGCGCAGCCTGCGCAG
CCGCCCTCGTCCTACTCGCTCCCCCTCCTGCTGTGCAAAGTGTTCAGGTG
GCCGGATCTCAGGCATTCCTCGGAAGTCAAGAGGCTGTGTTGCTGTGAAT
CTTACGGGAAGATCAACCCCGAGCTGGTGTGCTGCAACCCCCATCACCTT
AGCCGACTCTGCGAACTAGAGTCTCCCCCCCCTCCTTACTCCAGATACCC
GATGGATTTTCTCAAACCAACTGCAGACTGTCCAGATGCTGTGCCTTCCT
CCGCTGAAACAGGGGGAACGAATTATCTGGCCCCTGGGGGGCTTTCAGAT
TCCCAACTTCTTCTGGAGCCTGGGGATCGGTCACACTGGTGCGTGGTGGC
ATACTGGGAGGAGAAGACGAGAGTGGGGAGGCTCTACTGTGTCCAGGAGC
CCTCTCTGGATATCTTCTATGATCTACCTCAGGGGAATGGCTTTTGCCTC
GGACAGCTCAATTCGGACAACAAGAGTCAGCTGGTGCAGAAGGTGCGGAG
CAAAATCGGCTGCGGCATCCAGCTGACGCGGGAGGTGGATGGTGTGTGGG
TGTACAACCGCAGCAGTTACCCCATCTTCATCAAGTCCGCCACACTGGAC
AACCCGGACTCCAGGACGCTGTTGGTACACAAGGTGTTCCCCGGTTTCTC
CATCAAGGCTTTCGACTACGAGAAGGCGTACAGCCTGCAGCGGCCCAATG
ACCACGAGTTTATGCAGCAGCCGTGGACGGGCTTTACCGTGCAGATCAGC
TTTGTGAAGGGCTGGGGTCAGTGCTACACCCGCCAGTTCATCAGCAGCTG
CCCGTGCTGGCTAGAGGTCATCTTCAACAGCCGGTAGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMFRTKRSALVRRL
WRSRAPGGEDEEEGAGGGGGGGELRGEGATDSRAHGAGGGGPGRAGCCLG
KAVRGAKGHHHPHPPAAGAGAAGGAEADLKALTHSVLKKLKERQLELLLQ
AVESRGGTRTACLLLPGRLDCRLGPGAPAGAQPAQPPSSYSLPLLLCKVF
RWPDLRHSSEVKRLCCCESYGKINPELVCCNPHHLSRLCELESPPPPYSR
YPMDFLKPTADCPDAVPSSAETGGTNYLAPGGLSDSQLLLEPGDRSHWCV
VAYWEEKTRVGRLYCVQEPSLDIFYDLPQGNGFCLGQLNSDNKSQLVQKV
RSKIGCGIQLTREVDGVWVYNRSSYPIFIKSATLDNPDSRTLLVHKVFPG
FSIKAFDYEKAYSLQRPNDHEFMQQPWTGFTVQISFVKGWGQCYTRQFIS
SCPCWLEVIFNSR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12448
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 73841 Daltons