cDNA Clone SMAD7

• Plasmid: pGEX6P-1-SMAD7
• Parent Plasmid: pGEX6P1
• Expressed: GST-SMAD7
• Genbank: O15105
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTTCAGGACCAAACGATCTGCGCTCGTCCGGCGTCTCTGGAG
GAGCCGTGCGCCCGGCGGCGAGGACGAGGAGGAGGGCGCAGGGGGAGGTG
GAGGAGGAGGCGAGCTGCGGGGAGAAGGGGCGACGGACAGCCGAGCGCAT
GGGGCCGGTGGCGGCGGCCCGGGCAGGGCTGGATGCTGCCTGGGCAAGGC
GGTGCGAGGTGCCAAAGGTCACCACCATCCCCACCCGCCAGCCGCGGGCG
CCGGCGCGGCCGGGGGCGCCGAGGCGGATCTGAAGGCGCTCACGCACTCG
GTGCTCAAGAAACTGAAGGAGCGGCAGCTGGAGCTGCTGCTCCAGGCCGT
GGAGTCCCGCGGCGGGACGCGCACCGCGTGCCTCCTGCTGCCCGGCCGCC
TGGACTGCAGGCTGGGCCCGGGGGCGCCCGCCGGCGCGCAGCCTGCGCAG
CCGCCCTCGTCCTACTCGCTCCCCCTCCTGCTGTGCAAAGTGTTCAGGTG
GCCGGATCTCAGGCATTCCTCGGAAGTCAAGAGGCTGTGTTGCTGTGAAT
CTTACGGGAAGATCAACCCCGAGCTGGTGTGCTGCAACCCCCATCACCTT
AGCCGACTCTGCGAACTAGAGTCTCCCCCCCCTCCTTACTCCAGATACCC
GATGGATTTTCTCAAACCAACTGCAGACTGTCCAGATGCTGTGCCTTCCT
CCGCTGAAACAGGGGGAACGAATTATCTGGCCCCTGGGGGGCTTTCAGAT
TCCCAACTTCTTCTGGAGCCTGGGGATCGGTCACACTGGTGCGTGGTGGC
ATACTGGGAGGAGAAGACGAGAGTGGGGAGGCTCTACTGTGTCCAGGAGC
CCTCTCTGGATATCTTCTATGATCTACCTCAGGGGAATGGCTTTTGCCTC
GGACAGCTCAATTCGGACAACAAGAGTCAGCTGGTGCAGAAGGTGCGGAG
CAAAATCGGCTGCGGCATCCAGCTGACGCGGGAGGTGGATGGTGTGTGGG
TGTACAACCGCAGCAGTTACCCCATCTTCATCAAGTCCGCCACACTGGAC
AACCCGGACTCCAGGACGCTGTTGGTACACAAGGTGTTCCCCGGTTTCTC
CATCAAGGCTTTCGACTACGAGAAGGCGTACAGCCTGCAGCGGCCCAATG
ACCACGAGTTTATGCAGCAGCCGTGGACGGGCTTTACCGTGCAGATCAGC
TTTGTGAAGGGCTGGGGTCAGTGCTACACCCGCCAGTTCATCAGCAGCTG
CCCGTGCTGGCTAGAGGTCATCTTCAACAGCCGGTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMFRTKRSALVRRLWRSRAP
GGEDEEEGAGGGGGGGELRGEGATDSRAHGAGGGGPGRAGCCLGKAVRGA
KGHHHPHPPAAGAGAAGGAEADLKALTHSVLKKLKERQLELLLQAVESRG
GTRTACLLLPGRLDCRLGPGAPAGAQPAQPPSSYSLPLLLCKVFRWPDLR
HSSEVKRLCCCESYGKINPELVCCNPHHLSRLCELESPPPPYSRYPMDFL
KPTADCPDAVPSSAETGGTNYLAPGGLSDSQLLLEPGDRSHWCVVAYWEE
KTRVGRLYCVQEPSLDIFYDLPQGNGFCLGQLNSDNKSQLVQKVRSKIGC
GIQLTREVDGVWVYNRSSYPIFIKSATLDNPDSRTLLVHKVFPGFSIKAF
DYEKAYSLQRPNDHEFMQQPWTGFTVQISFVKGWGQCYTRQFISSCPCWL
EVIFNSR

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12443
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 73247 Daltons