cDNA Clone CRTC2

• Synonyms: TORC2
• Plasmid: pEBG6P-CRTC2 S171A
• Parent Plasmid: pEBG6P
• Expressed: GST-CRTC2 S171A
• Genbank: NM_181715
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCGACGTCGGGGGCGAACGGGCCTGGTTCGGCCACGGC
CTCGGCTTCCAATCCGCGCAAATTTAGTGAGAAGATTGCGCTGCAGAAGC
AGCGTCAGGCCGAGGAGACGGCGGCCTTCGAGGAGGTGATGATGGACATC
GGCTCCACCCGGTTACAGGCCCAAAAACTGCGACTGGCATACACAAGGAG
CTCTCATTATGGTGGGTCTCTGCCCAATGTTAACCAGATTGGCTCTGGCC
TGGCCGAGTTCCAGAGCCCCCTCCACTCACCTTTGGATTCATCTCGGAGC
ACTCGGCACCATGGGCTGGTGGAACGGGTGCAGCGAGATCCTCGAAGAAT
GGTGTCCCCACTTCGCCGATACACCCGCCACATTGACAGCTCTCCCTATA
GTCCTGCCTACTTATCTCCTCCCCCAGAGTCTAGCTGGCGAAGGACGATG
GCCTGGGGCAATTTCCCTGCAGAGAAGGGGCAGTTGTTTCGACTACCATC
TGCACTTAACAGGACAAGCGCTGACTCTGCCCTTCATACAAGTGTGATGA
ACCCCAGTCCCCAGGATACCTACCCAGGCCCCACACCTCCCAGCATCCTG
CCCAGCCGACGTGGGGGTATTCTGGATGGTGAAATGGACCCCAAAGTACC
TGCTATTGAGGAGAACTTGCTAGATGACAAGCATTTGCTGAAGCCATGGG
ATGCTAAGAAGCTATCCTCATCCTCTTCCCGACCTCGGTCCTGTGAAGTC
CCTGGAATTAACATCTTTCCATCTCCTGACCAGCCTGCCAATGTGCCTGT
CCTCCCACCTGCCATGAACACGGGGGGCTCCCTACCTGACCTCACCAACC
TGCACTTTCCCCCACCACTGCCCACCCCCCTGGACCCTGAAGAGACAGCC
TACCCTAGCCTGAGTGGGGGCAACAGTACCTCCAATTTGACCCACACCAT
GACTCACCTGGGCATCAGCAGGGGCATGGGCCTGGGCCCAGGCTATGATG
CACCAGGACTTCATTCACCTCTCAGCCACCCATCCCTGCAGTCCTCCCTA
AGCAATCCCAACCTCCAGGCTTCCCTGAGCAGTCCTCAGCCCCAGCTTCA
GGGCTCCCACAGCCACCCCTCTCTGCCTGCCTCCTCCTTGGCCCGCCATG
TACTGCCCACCACCTCCCTGGGCCACCCCTCACTCAGTGCTCCGGCTCTT
TCCTCCTCCTCTTCCTCCTCCTCCACTTCATCTCCTGTTTTGGGCGCCCC
CTCTTACCCTGCTTCTACCCCTGGGGCCTCCCCCCACCACCGCCGTGTGC
CCCTCAGCCCCCTGAGTTTGCTCGCGGGCCCAGCCGACGCCAGAAGGTCC
CAACAGCAGCTGCCCAAACAGTTTTCGCCAACAATGTCACCCACCTTGTC
TTCCATCACTCAGGGCGTCCCCCTGGATACCAGTAAACTGTCCACTGACC
AGCGGTTACCCCCATACCCATACAGCTCCCCAAGTCTGGTTCTGCCTACC
CAGCCCCACACCCCAAAGTCTCTACAGCAGCCAGGGCTGCCCTCTCAGTC
TTGTTCAGTGCAGTCCTCAGGTGGGCAGCCCCCAGGCAGGCAGTCTCATT
ATGGGACACCGTACCCACCTGGGCCCAGTGGGCATGGGCAACAGTCTTAC
CACCGGCCAATGAGTGACTTCAACCTGGGGAATCTGGAGCAGTTCAGCAT
GGAGAGCCCATCAGCCAGCCTGGTGCTGGATCCCCCTGGCTTTTCTGAAG
GGCCTGGATTTTTAGGGGGTGAGGGGCCAATGGGTGGCCCCCAGGATCCC
CACACCTTCAACCACCAGAACTTGACCCACTGTTCCCGCCATGGCTCAGG
GCCTAACATCATCCTCACAGGGGACTCCTCTCCAGGTTTCTCTAAGGAGA
TTGCAGCAGCCCTGGCCGGAGTGCCTGGCTTTGAGGTGTCAGCAGCTGGA
TTGGAGCTAGGGCTTGGGCTAGAAGATGAGCTGCGCATGGAGCCACTGGG
CCTGGAAGGGCTAAACATGCTGAGTGACCCCTGTGCCCTGCTGCCTGATC
CTGCTGTGGAGGAGTCATTCCGCAGTGACCGGCTCCAATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMATS
GANGPGSATASASNPRKFSEKIALQKQRQAEETAAFEEVMMDIGSTRLQA
QKLRLAYTRSSHYGGSLPNVNQIGSGLAEFQSPLHSPLDSSRSTRHHGLV
ERVQRDPRRMVSPLRRYTRHIDSSPYSPAYLSPPPESSWRRTMAWGNFPA
EKGQLFRLPSALNRTSADSALHTSVMNPSPQDTYPGPTPPSILPSRRGGI
LDGEMDPKVPAIEENLLDDKHLLKPWDAKKLSSSSSRPRSCEVPGINIFP
SPDQPANVPVLPPAMNTGGSLPDLTNLHFPPPLPTPLDPEETAYPSLSGG
NSTSNLTHTMTHLGISRGMGLGPGYDAPGLHSPLSHPSLQSSLSNPNLQA
SLSSPQPQLQGSHSHPSLPASSLARHVLPTTSLGHPSLSAPALSSSSSSS
STSSPVLGAPSYPASTPGASPHHRRVPLSPLSLLAGPADARRSQQQLPKQ
FSPTMSPTLSSITQGVPLDTSKLSTDQRLPPYPYSSPSLVLPTQPHTPKS
LQQPGLPSQSCSVQSSGGQPPGRQSHYGTPYPPGPSGHGQQSYHRPMSDF
NLGNLEQFSMESPSASLVLDPPGFSEGPGFLGGEGPMGGPQDPHTFNHQN
LTHCSRHGSGPNIILTGDSSPGFSKEIAAALAGVPGFEVSAAGLELGLGL
EDELRMEPLGLEGLNMLSDPCALLPDPAVEESFRSDRLQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12364
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.