cDNA Clone CRTC2

• Synonyms: TORC2
• Plasmid: pCMV-FLAG CRTC2 S171A
• Parent Plasmid: pCMV5
• Expressed: FLAG CRTC2 S171A
• Genbank: NM_181715
• Tag: FLAG
• Species: Human

Sequence of Insert

GAATTCGCCACCATGGACTACAAGGACGACGATGACAAGATGGCGACGTC
GGGGGCGAACGGGCCTGGTTCGGCCACGGCCTCGGCTTCCAATCCGCGCA
AATTTAGTGAGAAGATTGCGCTGCAGAAGCAGCGTCAGGCCGAGGAGACG
GCGGCCTTCGAGGAGGTGATGATGGACATCGGCTCCACCCGGTTACAGGC
CCAAAAACTGCGACTGGCATACACAAGGAGCTCTCATTATGGTGGGTCTC
TGCCCAATGTTAACCAGATTGGCTCTGGCCTGGCCGAGTTCCAGAGCCCC
CTCCACTCACCTTTGGATTCATCTCGGAGCACTCGGCACCATGGGCTGGT
GGAACGGGTGCAGCGAGATCCTCGAAGAATGGTGTCCCCACTTCGCCGAT
ACACCCGCCACATTGACAGCTCTCCCTATAGTCCTGCCTACTTATCTCCT
CCCCCAGAGTCTAGCTGGCGAAGGACGATGGCCTGGGGCAATTTCCCTGC
AGAGAAGGGGCAGTTGTTTCGACTACCATCTGCACTTAACAGGACAAGCG
CTGACTCTGCCCTTCATACAAGTGTGATGAACCCCAGTCCCCAGGATACC
TACCCAGGCCCCACACCTCCCAGCATCCTGCCCAGCCGACGTGGGGGTAT
TCTGGATGGTGAAATGGACCCCAAAGTACCTGCTATTGAGGAGAACTTGC
TAGATGACAAGCATTTGCTGAAGCCATGGGATGCTAAGAAGCTATCCTCA
TCCTCTTCCCGACCTCGGTCCTGTGAAGTCCCTGGAATTAACATCTTTCC
ATCTCCTGACCAGCCTGCCAATGTGCCTGTCCTCCCACCTGCCATGAACA
CGGGGGGCTCCCTACCTGACCTCACCAACCTGCACTTTCCCCCACCACTG
CCCACCCCCCTGGACCCTGAAGAGACAGCCTACCCTAGCCTGAGTGGGGG
CAACAGTACCTCCAATTTGACCCACACCATGACTCACCTGGGCATCAGCA
GGGGCATGGGCCTGGGCCCAGGCTATGATGCACCAGGACTTCATTCACCT
CTCAGCCACCCATCCCTGCAGTCCTCCCTAAGCAATCCCAACCTCCAGGC
TTCCCTGAGCAGTCCTCAGCCCCAGCTTCAGGGCTCCCACAGCCACCCCT
CTCTGCCTGCCTCCTCCTTGGCCCGCCATGTACTGCCCACCACCTCCCTG
GGCCACCCCTCACTCAGTGCTCCGGCTCTTTCCTCCTCCTCTTCCTCCTC
CTCCACTTCATCTCCTGTTTTGGGCGCCCCCTCTTACCCTGCTTCTACCC
CTGGGGCCTCCCCCCACCACCGCCGTGTGCCCCTCAGCCCCCTGAGTTTG
CTCGCGGGCCCAGCCGACGCCAGAAGGTCCCAACAGCAGCTGCCCAAACA
GTTTTCGCCAACAATGTCACCCACCTTGTCTTCCATCACTCAGGGCGTCC
CCCTGGATACCAGTAAACTGTCCACTGACCAGCGGTTACCCCCATACCCA
TACAGCTCCCCAAGTCTGGTTCTGCCTACCCAGCCCCACACCCCAAAGTC
TCTACAGCAGCCAGGGCTGCCCTCTCAGTCTTGTTCAGTGCAGTCCTCAG
GTGGGCAGCCCCCAGGCAGGCAGTCTCATTATGGGACACCGTACCCACCT
GGGCCCAGTGGGCATGGGCAACAGTCTTACCACCGGCCAATGAGTGACTT
CAACCTGGGGAATCTGGAGCAGTTCAGCATGGAGAGCCCATCAGCCAGCC
TGGTGCTGGATCCCCCTGGCTTTTCTGAAGGGCCTGGATTTTTAGGGGGT
GAGGGGCCAATGGGTGGCCCCCAGGATCCCCACACCTTCAACCACCAGAA
CTTGACCCACTGTTCCCGCCATGGCTCAGGGCCTAACATCATCCTCACAG
GGGACTCCTCTCCAGGTTTCTCTAAGGAGATTGCAGCAGCCCTGGCCGGA
GTGCCTGGCTTTGAGGTGTCAGCAGCTGGATTGGAGCTAGGGCTTGGGCT
AGAAGATGAGCTGCGCATGGAGCCACTGGGCCTGGAAGGGCTAAACATGC
TGAGTGACCCCTGTGCCCTGCTGCCTGATCCTGCTGTGGAGGAGTCATTC
CGCAGTGACCGGCTCCAATGAGCGGCCGCGCAAGGGCGAATTC

Amino Acid Sequence

MDYKDDDDKMATSGANGPGSATASASNPRKFSEKIALQKQRQAEETAAFE
EVMMDIGSTRLQAQKLRLAYTRSSHYGGSLPNVNQIGSGLAEFQSPLHSP
LDSSRSTRHHGLVERVQRDPRRMVSPLRRYTRHIDSSPYSPAYLSPPPES
SWRRTMAWGNFPAEKGQLFRLPSALNRTSADSALHTSVMNPSPQDTYPGP
TPPSILPSRRGGILDGEMDPKVPAIEENLLDDKHLLKPWDAKKLSSSSSR
PRSCEVPGINIFPSPDQPANVPVLPPAMNTGGSLPDLTNLHFPPPLPTPL
DPEETAYPSLSGGNSTSNLTHTMTHLGISRGMGLGPGYDAPGLHSPLSHP
SLQSSLSNPNLQASLSSPQPQLQGSHSHPSLPASSLARHVLPTTSLGHPS
LSAPALSSSSSSSSTSSPVLGAPSYPASTPGASPHHRRVPLSPLSLLAGP
ADARRSQQQLPKQFSPTMSPTLSSITQGVPLDTSKLSTDQRLPPYPYSSP
SLVLPTQPHTPKSLQQPGLPSQSCSVQSSGGQPPGRQSHYGTPYPPGPSG
HGQQSYHRPMSDFNLGNLEQFSMESPSASLVLDPPGFSEGPGFLGGEGPM
GGPQDPHTFNHQNLTHCSRHGSGPNIILTGDSSPGFSKEIAAALAGVPGF
EVSAAGLELGLGLEDELRMEPLGLEGLNMLSDPCALLPDPAVEESFRSDR
LQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12351
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal FLAG for mammalian expression.; ; Estimated MW of expressed protein : 74409 Daltons