cDNA Clone CRK3

• Plasmid: pFastbacDual 6P1 CYC6 6His CRK3
• Parent Plasmid: pFastbacDual 6P1
• Genbank: XP_829729.1 and XP_822746.1
• Tag: GST
• Species: Trypanosoma brucei TREU927

Sequence of Insert

GGATCCATGAATCCCACGGCACTTCGTGAGGTCTCAAACCTGTCCACAGG
TTCTGGAGATGTGAAGCAGCGACTTGCTCAGCGGCGCAGCGCTAATTTAG
AGTCATGTCGTTATCCTTACTCATCCAGCGCTGTGCGACACGGCTCCGGC
AGTTTGTTAAGCCTTCGCTCAAGTCTCTACAGCCCCGTAGGTACGGCAGG
GAAGGAGTCATCTGTAAGCCCTCATGCTGACCGGCTTCGTGAGAATAATC
CGGTATACTGCAGCGATGTTATTAAGGACATCACAACAATGTACATGGAA
AGTGAGAAGGAGGCTATATACGGTCAGGTACTTCCGTCACCCCGCTATTT
AACGTACCAGCCAGAGATAAACGAGAAAATGCGAATGATTCTCGTCGATT
GGCTTATTGACGTTCACCTAAAGTTTAAACTACATCCCGAGACGCTTTAC
CTCACGGTGAGCATCGTAGACCGCTACCTTTCTTCTGTTAACACGAGGCG
TACGACCGGACGGTACATACCGCGTTCAAAGCTGCAGTTAGTTGGTATCA
CCGCCATTCTTCTTGCTGCCAAGTACGAAGAGATATGGCCTCCGGAGGTG
AAGGAGTGCGTGTACATATGTGCCAATACCTACACTCGTGATGAGGTTAT
TCGCATGGAACGTGAGATGTGCACCGAGCTGTCCTTCCGCTTTACTGTTC
CAACGCCGTTTCCTTTCATTGTTCGATTACTTGACGTTGTTGAGGGCCTC
GAGCGGCAGCAGCAGACGCAACAGTTGTCACATCAGCTACAGCAGATCCA
ATCGCAGTCGCAATCTCAATCTCAACAGACACAAATGCAAGCATCACAGT
CACAACCACAATCTCTGTCGCGTTCCCGCTCACAGTCGCGTTCGCAGCCG
CGCTCTGATGAGGAATGCACGGCATATGTTCTACAGCTTCGGCACACCGC
TTTCTTTTTTCTTGACCACGGCATGCTTGATTACAAATGCCTTCAGTTCT
CCCCGTCGCAGCAAGCAAAGGCCGCCTTGTTTCTTGCACTAGTGACACTA
CACATGAAAGAGCAAGGCTCATCGTATGTGCTGAACAACGATATCATATG
GACTCGTCAGCTTCAGTACTACAGTAAGGCTCAAGTACGTGATTTCAAGG
CGTGCGCGAGGACCATGTTAGATTTTGTGAGTTACGTCTCCACAACGCGT
TACCAATCGGTGCGTCGCAAGTACAGCAGCTCAAAGTATGGTGAAGTGGC
TAAACTGACTATGCCTAGTGAAGTACCTGACTTTTGAGCGGCCGC

DNA sequence of CRK3 insert :
GCTAGCGAAACCATGCATCATCACCATCACCATATGACAATGCTTGGGGCGTTAACCGGTCGACAACTTTCCTCTGGTCTTAAGGAGCAGTTCGACCGCT
ATAATCGAATGGACATACTTGGAGAAGGAACGTATGGAGTTGTATACCGT
GCTGTTGACAGAGCAACGGGACAGATCGTCGCACTGAAGAAAGTGAGATT
AGATCGCACCGATGAGGGAATACCTCAAACAGCTCTTCGGGAAGTATCTA
TTTTGCAAGAAATCCATCACCCCAACATTGTTAATCTTCTAGATGTTATA
TGTGCCGATGGGAAGCTTTACTTGATATTTGAGTATGTTGATCATGATCT
CAAAAAGGCTCTCGAAAAGAGGGGTGGAGCATTCACGGGAACTACACTAA
AAAAGATAATTTACCAGCTACTCGAGGGACTTTCGTTTTGTCATCGGCAC
CGCATTGTCCATCGAGACCTAAAACCAGCAAACATACTTGTCACCACGGA
TAATTCCGTTAAAATAGCAGACTTTGGATTGGCTCGCGCTTTCCAGATTC
CGATGCACACTTACACTCACGAGGTTGTCACACTCTGGTATAGAGCGCCA
GAGATTCTCCTCGGTGAAAAGCACTACACCCCGGCAGTGGATATGTGGAG
CATTGGTTGTATTTTTGCTGAACTAGCTCGAGGTAAGGTACTCTTTCGTG
GTGACAGTAAGATTGGACAGTTATTTGAGATTTTTCAGGTGCTTGGCACC
CCAATGGATGCTGAGGGGTCGTGGTTGGGGGTGTCGTCTCTTCCAGACTA
TCGTGACGTCTTTCCAAAGTGGAATGGAAAACCCCTCACTCAGGTGTTAC
CAGCGCTTGACGGTGATGCCGTTGATCTGCTTTCTCAGATGCTGAGGTAT
AACCCTGCTGAACGTATTTCAGCCAAGGCGGCACTGCAACATCCATGGTT
TAGTGATGCCATGTTTTAGGCTAGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNPTALREVSNLSTGSGDV
KQRLAQRRSANLESCRYPYSSSAVRHGSGSLLSLRSSLYSPVGTAGKESS
VSPHADRLRENNPVYCSDVIKDITTMYMESEKEAIYGQVLPSPRYLTYQP
EINEKMRMILVDWLIDVHLKFKLHPETLYLTVSIVDRYLSSVNTRRTTGR
YIPRSKLQLVGITAILLAAKYEEIWPPEVKECVYICANTYTRDEVIRMER
EMCTELSFRFTVPTPFPFIVRLLDVVEGLERQQQTQQLSHQLQQIQSQSQ
SQSQQTQMQASQSQPQSLSRSRSQSRSQPRSDEECTAYVLQLRHTAFFFL
DHGMLDYKCLQFSPSQQAKAALFLALVTLHMKEQGSSYVLNNDIIWTRQL
QYYSKAQVRDFKACARTMLDFVSYVSTTRYQSVRRKYSSSKYGEVAKLTM
PSEVPDF*


Protein sequence. 1M of CRK3 is 8M of expressed protein :
MHHHHHHMTMLGALTGRQLSSGLKEQFDRYNRMDILGEGTYGVVYRAVDR
ATGQIVALKKVRLDRTDEGIPQTALREVSILQEIHHPNIVNLLDVICADG
KLYLIFEYVDHDLKKALEKRGGAFTGTTLKKIIYQLLEGLSFCHRHRIVH
RDLKPANILVTTDNSVKIADFGLARAFQIPMHTYTHEVVTLWYRAPEILL
GEKHYTPAVDMWSIGCIFAELARGKVLFRGDSKIGQLFEIFQVLGTPMDA
EGSWLGVSSLPDYRDVFPKWNGKPLTQVLPALDGDAVDLLSQMLRYNPAE
RISAKAALQHPWFSDAMF*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU12328
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST and 6His dual expression vector for baculovirus expression.; ; Estimated MW of CYC6 : 75787 Daltons; ; Estimated MW of CYC3 : 36010 Daltons; Protein Sequence. 1M of CYC is 232M of expressed protein :