cDNA Clone MKK7

• Plasmid: pGEX-4T-FLAG MKK7 D261A
• Parent Plasmid: pGEX4T1
• Expressed: GST-FLAG MKK7 D261A
• Genbank: NP_005034
• Tag: GST
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGACTACAAGGACGACGATGACAAGGCGGCGTCCTC
CCTGGAACAGAAGCTGTCCCGCCTGGAAGCAAAGCTGAAGCAGGAGAACC
GGGAGGCCCGGCGGAGGATCGACCTCAACCTGGATATCAGCCCCCAGCGG
CCCAGGCCCACCCTGCAGCTCCCGCTGGCCAACGATGGGGGCAGCCGCTC
GCCATCCTCAGAGAGCTCCCCGCAGCACCCCACGCCCCCCGCCCGGCCCC
GCCACATGCTGGGGCTCCCGTCAACCCTGTTCACACCCCGCAGCATGGAG
AGCATTGAGATTGACCAGAAGCTGCAGGAGATCATGAAGCAGACGGGCTA
CCTGACCATCGGGGGCCAGCGCTACCAGGCAGAAATCAACGACCTGGAGA
ACTTGGGCGAGATGGGCAGCGGCACCTGCGGCCAGGTGTGGAAGATGCGC
TTCCGGAAGACCGGCCACGTCATTGCCGTTAAGCAAATGCGGCGCTCCGG
GAACAAGGAGGAGAACAAGCGCATCCTCATGGACCTGGATGTGGTGCTGA
AGAGCCACGACTGCCCCTACATCGTGCAGTGCTTTGGGACGTTCATCACC
AACACGGACGTCTTCATCGCCATGGAGCTCATGGGCACCTGCGCTGAGAA
GCTCAAGAAGCGGATGCAGGGCCCCATCCCCGAGCGCATTCTGGGCAAGA
TGACAGTGGCGATTGTGAAGGCGCTGTACTACCTGAAGGAGAAGCACGGT
GTCATCCACCGCGACGTCAAGCCCTCCAACATCCTGCTGGACGAGCGGGG
CCAGATCAAGCTCTGCGCCTTCGGCATCAGCGGCCGCCTGGTGGACTCCA
AAGCCAAGACGCGGAGCGCCGGCTGTGCCGCCTACATGGCACCCGAGCGC
ATTGACCCCCCAGACCCCACCAAGCCGGACTATGACATCCGGGCCGACGT
ATGGAGCCTGGGCATCTCGTTGGTGGAGCTGGCAACAGGACAGTTTCCCT
ACAAGAACTGCAAGACGGACTTTGAGGTCCTCACCAAAGTCCTACAGGAA
GAGCCCCCGCTTCTGCCCGGACACATGGGCTTCTCGGGGGACTTCCAGTC
CTTCGTCAAAGACTGCCTTACTAAAGATCACAGGAAGAGACCAAAGTATA
ATAAGCTACTTGAACACAGCTTCATCAAGCGCTACGAGACGCTGGAGGTG
GACGTGGCGTCCTGGTTCAAGGATGTCATGGCGAAGACTGAGTCACCGCG
GACTAGCGGCGTCCTGAGCCAGCCCCACCTGCCCTTCTTCAGGTAGGAAT
TC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSATMDYKDDDDKAASSLEQKLSRLE
AKLKQENREARRRIDLNLDISPQRPRPTLQLPLANDGGSRSPSSESSPQH
PTPPARPRHMLGLPSTLFTPRSMESIEIDQKLQEIMKQTGYLTIGGQRYQ
AEINDLENLGEMGSGTCGQVWKMRFRKTGHVIAVKQMRRSGNKEENKRIL
MDLDVVLKSHDCPYIVQCFGTFITNTDVFIAMELMGTCAEKLKKRMQGPI
PERILGKMTVAIVKALYYLKEKHGVIHRDVKPSNILLDERGQIKLCAFGI
SGRLVDSKAKTRSAGCAAYMAPERIDPPDPTKPDYDIRADVWSLGISLVE
LATGQFPYKNCKTDFEVLTKVLQEEPPLLPGHMGFSGDFQSFVKDCLTKD
HRKRPKYNKLLEHSFIKRYETLEVDVASWFKDVMAKTESPRTSGVLSQPH
LPFFR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12319
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct with N terminal GST tag. Kinase dead mutation.; ; Estimated MW of expressed protein : 74898 Daltons