cDNA Clone CRK3

• Plasmid: pFBHTb CRK3 T brucei
• Parent Plasmid: pFastBac HTb
• Genbank: XP_822746.1
• Species: Trypanosoma brucei TREU927

Sequence of Insert

GGATCCATGACAATGCTTGGGGCGTTAACCGGTCGACAACTTTCCTCTGG
TCTTAAGGAGCAGTTCGACCGCTATAATCGAATGGACATACTTGGAGAAG
GAACGTATGGAGTTGTATACCGTGCTGTTGACAGAGCAACGGGACAGATC
GTCGCACTGAAGAAAGTGAGATTAGATCGCACCGATGAGGGAATACCTCA
AACAGCTCTTCGGGAAGTATCTATTTTGCAAGAAATCCATCACCCCAACA
TTGTTAATCTTCTAGATGTTATATGTGCCGATGGGAAGCTTTACTTGATA
TTTGAGTATGTTGATCATGATCTCAAAAAGGCTCTCGAAAAGAGGGGTGG
AGCATTCACGGGAACTACACTAAAAAAGATAATTTACCAGCTACTCGAGG
GACTTTCGTTTTGTCATCGGCACCGCATTGTCCATCGAGACCTAAAACCA
GCAAACATACTTGTCACCACGGATAATTCCGTTAAAATAGCAGACTTTGG
ATTGGCTCGCGCTTTCCAGATTCCGATGCACACTTACACTCACGAGGTTG
TCACACTCTGGTATAGAGCGCCAGAGATTCTCCTCGGTGAAAAGCACTAC
ACCCCGGCAGTGGATATGTGGAGCATTGGTTGTATTTTTGCTGAACTAGC
TCGAGGTAAGGTACTCTTTCGTGGTGACAGTAAGATTGGACAGTTATTTG
AGATTTTTCAGGTGCTTGGCACCCCAATGGATGCTGAGGGGTCGTGGTTG
GGGGTGTCGTCTCTTCCAGACTATCGTGACGTCTTTCCAAAGTGGAATGG
AAAACCCCTCACTCAGGTGTTACCAGCGCTTGACGGTGATGCCGTTGATC
TGCTTTCTCAGATGCTGAGGTATAACCCTGCTGAACGTATTTCAGCCAAG
GCGGCACTGCAACATCCATGGTTTAGTGATGCCATGTTTTAGGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMTMLGALTGRQLSSGLKEQFDR
YNRMDILGEGTYGVVYRAVDRATGQIVALKKVRLDRTDEGIPQTALREVS
ILQEIHHPNIVNLLDVICADGKLYLIFEYVDHDLKKALEKRGGAFTGTTL
KKIIYQLLEGLSFCHRHRIVHRDLKPANILVTTDNSVKIADFGLARAFQI
PMHTYTHEVVTLWYRAPEILLGEKHYTPAVDMWSIGCIFAELARGKVLFR
GDSKIGQLFEIFQVLGTPMDAEGSWLGVSSLPDYRDVFPKWNGKPLTQVL
PALDGDAVDLLSQMLRYNPAERISAKAALQHPWFSDAMF*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU12318
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for baculovirus expression. Differs to genbank at D18E, E226K and S264N. This is the same as provided template.; ; Estimated MW of expressed protein : 38427 Daltons