cDNA Clone PLK

• Plasmid: pGEX-5X-tb PLK
• Parent Plasmid: pGEX5X
• Genbank: XP_846254.1
• Species: Trypanosoma brucei TREU927

Sequence of Insert

GGATCCACGCAACCGCTGAGACGTGTGAAACGCCGTCGAGTTCGCGGCGC
CCTCCAAATGACAGGCCGGACTTTCGTGAGGGTTCAACTCTTAAGGAGTT
TGATAAAAGTGGCCGTCTCGTGGGATATTTCCGTTGCGGAAGGATGCTCG
GGCGTGGTGGCTTTGCAAAGTGTTATGAAGTGGAGCAGGGTGGCGATACG
TATGCGTTGAAGGTTGTGGACCGCTCACTACTGCAGAAAACTAAGACACT
ACAGAAACTCCACTCTGAGATATCCATTCACCGCCGCATGAAGCACAAAC
ACATTGTTAACTTCATACGGACCTTCCATGACGACTGGAATGTTTATATT
CTGTTGGAGAAGTGCAGCAATCAGACGCTGATGGAAATATTGAAGCGCCG
GCAACGTTTCAGTGTGCCAGAGACACAATACATTGCATTGCAGTCGCTTT
CCGCCATTCAATATATGCATGAGCAATGTGTTATTCATCGTGACCTGAAA
CTTGGTAACATAATGATGGATGCAAATATGAACGTGAAGATTGGGGACTT
CGGTTTGGCTGCTGAGTTGCAGTACGACGGGGAGCGGAAACGCACTATTT
GTGGCACGCCCAATTATATCGCACCGGAGATCATTGAAGGTTCGCGCGAG
GGACATAGTTACGAAGTGGATGTGTGGTCGCTCGGTGTGATCCTCTACAC
GCTGTTGGTTGGAGAGCCGCCGTTTCAGACATCAGATGTGAAGGCCACTT
ACCGTCGTATTAGGCAATGCCGGTATGAATTTCCTTCACATGTAGATGTG
CCAGAGAGTGGGAAGGAACTTATTCACAGTATTCTGCAGAGCCGCCCCGA
CCAACGGCCGACGTTGCTGGAAATTCGTTCACATCCATTTTTCCGCCTCC
CGCCGCCACCGACGACAGCACCTACCACACTCTTCTACAGTTCACGCCGC
CGTCAGCATTCGGATGACCCCCGAGGACATGCGCAGGGACCACTTCCACT
TCGCCGCCAAAAATCAGGGGACATTCAGGCGGCGTTGCAGAAACAAACAC
CGCAACGCCGTCAACCGCAGTCGCAGCCCAAATCAGTTGAAGCTGTTCGC
TGCATTTCGAGTCCGCGTGTGTCCCGCGAAGTGCTTCAACCTATCAGCAC
AAATCTACCCAAAACGGACCGATACCACCTAAAACCCAGCTGCCCGGCGG
TAGCTTCCGCAAGGTTTCATGGGGCTTTGGGCGGTGACTGCAACAACAAC
AACAACAACAATAACAATAACATCAACAACAACAACAACAACAACATTAA
CAGTGCTGCGGTGAGCATCCCGTCACCTCGTGCAGATGCGATTCGACCCC
TCACGCAAGTTGCCGCACCGGGCGGTGCTGATGAATCCGCGGAAACCACT
GCAACAACTCCGCGTGTGCAGCTGAGACCTCACCCAGCTATAGAGGAAGA
GGAGAAGAATGAACTCACCGCCGTGCATGATCAACTTCATCAGACGTTAC
GGGAAATAGGCGACATTTCCCCACGTGAGGTTGCCACCCGCACACAAACC
AAACGGTCACCGCAAGTGGCTCCCTTGCCAGTGGCGAACTCACCCACAAC
ATCTACAGTTCCTGCATTAACGCCCAGATGCGAAGACGAGCCGGCGCCAT
CGTTGCCACTTCCCACTGTTTGGGTGACGAGTTTCGCGGATTTCTCCGAA
AAATACGGGTTGTGTTACCGGTTGAGCACCGGCCATACCGGTGTGCACTT
CAACGACTCCACTAAAATGGTGTGGGAGCCCATTACAAACCGTGTAGAGT
ATTATATGCGAGTGAAGGAAGTTGTTGCGCGTGGTAATGCCAACGTCCTG
CAGGCGCGAGATCAACAACATGCGTTTCACATGGATATGTTCCCTGAAAG
CCTGACCAAGAAGGTGACATTGATCAAATACTTTAAATCATACCTCTCAC
GAACGCGTAATTCTCATACCAACGTGGAGGTTGTACGGTGCTCACCATAT
GTGTCCGAAGTTACTCCGTCACTTAGCGGACCGCACATGATTGAAGATAT
TGTATATGTCAAGCGCTGGTTGATCACACCACAAGCAATTATATTCCGTC
TATCCAACAAAACTATTCAGGTTTGTTTTCATGATAAGGCGGAGGTTATT
CTAAGTTCGGAGTCGCGTGTTGTTACATATACAGAACCAACTGGCAACCG
CGTGACAATGTCGCTATCGTCTGTTGCGACACGCTCTAGAGAAATTGCTG
CACGGCTTCGTTACACCAAAGACATCCTCAGCGAGCTCATACAAAACCGT
GATATTTAGCTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLIEGRGIHATAETCETPSSSRRPPNDRPDF
REGSTLKEFDKSGRLVGYFRCGRMLGRGGFAKCYEVEQGGDTYALKVVDR
SLLQKTKTLQKLHSEISIHRRMKHKHIVNFIRTFHDDWNVYILLEKCSNQ
TLMEILKRRQRFSVPETQYIALQSLSAIQYMHEQCVIHRDLKLGNIMMDA
NMNVKIGDFGLAAELQYDGERKRTICGTPNYIAPEIIEGSREGHSYEVDV
WSLGVILYTLLVGEPPFQTSDVKATYRRIRQCRYEFPSHVDVPESGKELI
HSILQSRPDQRPTLLEIRSHPFFRLPPPPTTAPTTLFYSSRRRQHSDDPR
GHAQGPLPLRRQKSGDIQAALQKQTPQRRQPQSQPKSVEAVRCISSPRVS
REVLQPISTNLPKTDRYHLKPSCPAVASARFHGALGGDCNNNNNNNNNNI
NNNNNNNINSAAVSIPSPRADAIRPLTQVAAPGGADESAETTATTPRVQL
RPHPAIEEEEKNELTAVHDQLHQTLREIGDISPREVATRTQTKRSPQVAP
LPVPNSPTTSTVPALTPRCEDEPAPSLPLPTVWVTSFADFSEKYGLCYRL
STGHTGVHFNDSTKMVWEPITNRVEYYMRVKEVVARGNANVLQARDQQHA
FHMDMFPESLTKKVTLIKYFKSYLSRTRNSHTNVEVVRCSPYVSEVTPSL
SGPHMIEDIVYVKRWLITPQAIIFRLSNKTIQVCFHDKAEVILSSESRVV
TYTEPTGNRVTMSLSSVATRSREIAARLRYTKDILSELIQNRDI

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12283
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 113284 Daltons