cDNA Clone RIPK2

• Plasmid: pEBG6P-RIPK2 S174A S176A
• Parent Plasmid: pEBG6P
• Expressed: GST-RIPK2 S174A S176A
• Genbank: NP_003812
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCCAACGGGGAGGCCATCTGCAGCGCCCTGCCCACCATTCCCTA
CCACAAACTCGCCGACCTGCGCTACCTGAGCCGCGGCGCCTCTGGCACTG
TGTCGTCCGCCCGCCACGCAGACTGGCGCGTCCAGGTGGCCGTGAAGCAC
CTGCACATCCACACTCCGCTGCTCGACAGTGAAAGAAAGGATGTCTTAAG
AGAAGCTGAAATTTTACACAAAGCTAGATTTAGTTACATTCTTCCAATTT
TGGGAATTTGCAATGAGCCTGAATTTTTGGGAATAGTTACTGAATACATG
CCAAATGGATCATTAAATGAACTCCTACATAGGAAAACTGAATATCCTGA
TGTTGCTTGGCCATTGAGATTTCGCATCCTGCATGAAATTGCCCTTGGTG
TAAATTACCTGCACAATATGACTCCTCCTTTACTTCATCATGACTTGAAG
ACTCAGAATATCTTATTGGACAATGAATTTCATGTTAAGATTGCAGATTT
TGGTTTATCAAAGTGGCGCATGATGGCCCTCGCACAGTCACGAAGTAGCA
AATCTGCACCAGAAGGAGGGACAATTATCTATATGCCACCTGAAAACTAT
GAACCTGGACAAAAATCAAGGGCCAGTATCAAGCACGATATATATAGCTA
TGCAGTTATCACATGGGAAGTGTTATCCAGAAAACAGCCTTTTGAAGATG
TCACCAATCCTTTGCAGATAATGTATAGTGTGTCACAAGGACATCGACCT
GTTATTAATGAAGAAAGTTTGCCATATGATATACCTCACCGAGCACGTAT
GATCTCTCTAATAGAAAGTGGATGGGCACAAAATCCAGATGAAAGACCAT
CTTTCTTAAAATGTTTAATAGAACTTGAACCAGTTTTGAGAACATTTGAA
GAGATAACTTTTCTTGAAGCTGTTATTCAGCTAAAGAAAACAAAGTTACA
GAGTGTTTCAAGTGCCATTCACCTATGTGACAAGAAGAAAATGGAATTAT
CTCTGAACATACCTGTAAATCATGGTCCACAAGAGGAATCATGTGGATCC
TCTCAGCTCCATGAAAATAGTGGTTCTCCTGAAACTTCAAGGTCCCTGCC
AGCTCCTCAAGACAATGATTTTTTATCTAGAAAAGCTCAAGACTGTTATT
TTATGAAGCTGCATCACTGTCCTGGAAATCACAGTTGGGATAGCACCATT
TCTGGATCTCAAAGGGCTGCATTCTGTGATCACAAGACCACTCCATGCTC
TTCAGCAATAATAAATCCACTCTCAACTGCAGGAAACTCAGAACGTCTGC
AGCCTGGTATAGCCCAGCAGTGGATCCAGAGCAAAAGGGAAGACATTGTG
AACCAAATGACAGAAGCCTGCCTTAACCAGTCGCTAGATGCCCTTCTGTC
CAGGGACTTGATCATGAAAGAGGACTATGAACTTGTTAGTACCAAGCCTA
CAAGGACCTCAAAAGTCAGACAATTACTAGACACTACTGACATCCAAGGA
GAAGAATTTGCCAAAGTTATAGTACAAAAATTGAAAGATAACAAACAAAT
GGGTCTTCAGCCTTACCCGGAAATACTTGTGGTTTCTAGATCACCATCTT
TAAATTTACTTCAAAATAAAAGCATGTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAANGEA
ICSALPTIPYHKLADLRYLSRGASGTVSSARHADWRVQVAVKHLHIHTPL
LDSERKDVLREAEILHKARFSYILPILGICNEPEFLGIVTEYMPNGSLNE
LLHRKTEYPDVAWPLRFRILHEIALGVNYLHNMTPPLLHHDLKTQNILLD
NEFHVKIADFGLSKWRMMALAQSRSSKSAPEGGTIIYMPPENYEPGQKSR
ASIKHDIYSYAVITWEVLSRKQPFEDVTNPLQIMYSVSQGHRPVINEESL
PYDIPHRARMISLIESGWAQNPDERPSFLKCLIELEPVLRTFEEITFLEA
VIQLKKTKLQSVSSAIHLCDKKKMELSLNIPVNHGPQEESCGSSQLHENS
GSPETSRSLPAPQDNDFLSRKAQDCYFMKLHHCPGNHSWDSTISGSQRAA
FCDHKTTPCSSAIINPLSTAGNSERLQPGIAQQWIQSKREDIVNQMTEAC
LNQSLDALLSRDLIMKEDYELVSTKPTRTSKVRQLLDTTDIQGEEFAKVI
VQKLKDNKQMGLQPYPEILVVSRSPSLNLLQNKSM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12127
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express RICK with N terminal GST.; Sequence change compared with sequence NP_003812: t1200a (silent in G400). This change present in EST sequences.; ; Estimated MW of expressed protein : 89234 Daltons