cDNA Clone RAD51

• Plasmid: pGEX-4T-RAD51 dont hand out
• Parent Plasmid: pGEX2TK
• Expressed: GST-RAD51 dont hand out
• Genbank: NM_002875
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCCCCATGGCAATGCAGATGCAGCTTGAAGCAAATGCAGATACTTC
AGTGGAAGAAGAAAGCTTTGGCCCACAACCCATTTCACGGTTAGAGCAGT
GTGGCATAAATGCCAACGATGTGAAGAAATTGGAAGAAGCTGGATTCCAT
ACTGTGGAGGCTGTTGCCTATGCGCCAAAGAAGGAGCTAATAAATATTAA
GGGAATTAGTGAAGCCAAAGCTGATAAAATTCTGGCTGAGGCAGCTAAAT
TAGTTCCAATGGGTTTCACCACTGCAACTGAATTCCACCAAAGGCGGTCA
GAGATCATACAGATTACTACTGGCTCCAAAGAGCTTGACAAACTACTTCA
AGGTGGAATTGAGACTGGATCTATCACAGAAATGTTTGGAGAATTCCGAA
CTGGGAAGACCCAGATCTGTCATACGCTAGCTGTCACCTGCCAGCTTCCC
ATTGACCGGGGTGGAGGTGAAGGAAAGGCCATGTACATTGACACTGAGGG
TACCTTTAGGCCAGAACGGCTGCTGGCAGTGGCTGAGAGGTATGGTCTCT
CTGGCAGTGATGTCCTGGATAATGTAGCATATGCTCGAGCGTTCAACACA
GACCACCAGACCCAGCTCCTTTATCAAGCATCAGCCATGATGGTAGAATC
TAGGTATGCACTGCTTATTGTAGACAGTGCCACCGCCCTTTACAGAACAG
ACTACTCGGGTCGAGGTGAGCTTTCAGCCAGGCAGATGCACTTGGCCAGG
TTTCTGCGGATGCTTCTGCGACTCGCTGATGAGTTTGGTGTAGCAGTGGT
AATCACTAATCAGGTGGTAGCTCAAGTGGATGGAGCAGCGATGTTTGCTG
CTGATCCCAAAAAACCTATTGGAGGAAATATCATCGCCCATGCATCAACA
ACCAGATTGTATCTGAGGAAAGGAAGAGGGGAAACCAGAATCTGCAAAAT
CTACGACTCTCCCTGTCTTCCTGAAGCTGAAGCTATGTTCGCCATTAATG
CAGATGGAGTGGGAGATGCCAAAGACTGAATCATTGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSPMAMQMQLEANADTSVEEESFGPQ
PISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADK
ILAEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETGSIT
EMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYIDTEGTFRPERLLA
VAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDS
ATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQV
DGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEA
EAMFAINADGVGDAKD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12114
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion . Supplied via MRC – not owned by University of Dundee.; ; Estimated MW of expressed protein : 63352 Daltons