cDNA Clone RIPK2

• Plasmid: pAS2.6 RIPK2
• Parent Plasmid: pAS2.6
• Expressed: GAL4 DNA BD-RIPK2
• Genbank: NP_003812
• Tag: GAL4 DNA-BD
• Species: Human

Sequence of Insert

GCGGCCGCCAACGGGGAGGCCATCTGCAGCGCCCTGCCCACCATTCCCTA
CCACAAACTCGCCGACCTGCGCTACCTGAGCCGCGGCGCCTCTGGCACTG
TGTCGTCCGCCCGCCACGCAGACTGGCGCGTCCAGGTGGCCGTGAAGCAC
CTGCACATCCACACTCCGCTGCTCGACAGTGAAAGAAAGGATGTCTTAAG
AGAAGCTGAAATTTTACACAAAGCTAGATTTAGTTACATTCTTCCAATTT
TGGGAATTTGCAATGAGCCTGAATTTTTGGGAATAGTTACTGAATACATG
CCAAATGGATCATTAAATGAACTCCTACATAGGAAAACTGAATATCCTGA
TGTTGCTTGGCCATTGAGATTTCGCATCCTGCATGAAATTGCCCTTGGTG
TAAATTACCTGCACAATATGACTCCTCCTTTACTTCATCATGACTTGAAG
ACTCAGAATATCTTATTGGACAATGAATTTCATGTTAAGATTGCAGATTT
TGGTTTATCAAAGTGGCGCATGATGTCCCTCTCACAGTCACGAAGTAGCA
AATCTGCACCAGAAGGAGGGACAATTATCTATATGCCACCTGAAAACTAT
GAACCTGGACAAAAATCAAGGGCCAGTATCAAGCACGATATATATAGCTA
TGCAGTTATCACATGGGAAGTGTTATCCAGAAAACAGCCTTTTGAAGATG
TCACCAATCCTTTGCAGATAATGTATAGTGTGTCACAAGGACATCGACCT
GTTATTAATGAAGAAAGTTTGCCATATGATATACCTCACCGAGCACGTAT
GATCTCTCTAATAGAAAGTGGATGGGCACAAAATCCAGATGAAAGACCAT
CTTTCTTAAAATGTTTAATAGAACTTGAACCAGTTTTGAGAACATTTGAA
GAGATAACTTTTCTTGAAGCTGTTATTCAGCTAAAGAAAACAAAGTTACA
GAGTGTTTCAAGTGCCATTCACCTATGTGACAAGAAGAAAATGGAATTAT
CTCTGAACATACCTGTAAATCATGGTCCACAAGAGGAATCATGTGGATCC
TCTCAGCTCCATGAAAATAGTGGTTCTCCTGAAACTTCAAGGTCCCTGCC
AGCTCCTCAAGACAATGATTTTTTATCTAGAAAAGCTCAAGACTGTTATT
TTATGAAGCTGCATCACTGTCCTGGAAATCACAGTTGGGATAGCACCATT
TCTGGATCTCAAAGGGCTGCATTCTGTGATCACAAGACCACTCCATGCTC
TTCAGCAATAATAAATCCACTCTCAACTGCAGGAAACTCAGAACGTCTGC
AGCCTGGTATAGCCCAGCAGTGGATCCAGAGCAAAAGGGAAGACATTGTG
AACCAAATGACAGAAGCCTGCCTTAACCAGTCGCTAGATGCCCTTCTGTC
CAGGGACTTGATCATGAAAGAGGACTATGAACTTGTTAGTACCAAGCCTA
CAAGGACCTCAAAAGTCAGACAATTACTAGACACTACTGACATCCAAGGA
GAAGAATTTGCCAAAGTTATAGTACAAAAATTGAAAGATAACAAACAAAT
GGGTCTTCAGCCTTACCCGGAAATACTTGTGGTTTCTAGATCACCATCTT
TAAATTTACTTCAAAATAAAAGCATGTAAGCGGCCGC

Amino Acid Sequence

MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLT
RAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQD
NVNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPVL
QYPALTHMGSPEFGRRAAANGEAICSALPTIPYHKLADLRYLSRGASGTV
SSARHADWRVQVAVKHLHIHTPLLDSERKDVLREAEILHKARFSYILPIL
GICNEPEFLGIVTEYMPNGSLNELLHRKTEYPDVAWPLRFRILHEIALGV
NYLHNMTPPLLHHDLKTQNILLDNEFHVKIADFGLSKWRMMSLSQSRSSK
SAPEGGTIIYMPPENYEPGQKSRASIKHDIYSYAVITWEVLSRKQPFEDV
TNPLQIMYSVSQGHRPVINEESLPYDIPHRARMISLIESGWAQNPDERPS
FLKCLIELEPVLRTFEEITFLEAVIQLKKTKLQSVSSAIHLCDKKKMELS
LNIPVNHGPQEESCGSSQLHENSGSPETSRSLPAPQDNDFLSRKAQDCYF
MKLHHCPGNHSWDSTISGSQRAAFCDHKTTPCSSAIINPLSTAGNSERLQ
PGIAQQWIQSKREDIVNQMTEACLNQSLDALLSRDLIMKEDYELVSTKPT
RTSKVRQLLDTTDIQGEEFAKVIVQKLKDNKQMGLQPYPEILVVSRSPSL
NLLQNKSM*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU8861
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast 2 Hybrid vector.; N.B. sequence change compared with sequence NP_003812: t1200a (silent in G400). This change present in EST sequences.; ; Estimated MW of expressed protein : 80262 Daltons