cDNA Clone RIPK2

• Plasmid: pGEX6P-2-RIPK2 K47A
• Parent Plasmid: pGEX6P2
• Expressed: GST-RIPK2 K47A
• Genbank: NP_003812
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGAACGGGGAGGCCATCTGCAGCGCCCTGCCCACCATTCCCTA
CCACAAACTCGCCGACCTGCGCTACCTGAGCCGCGGCGCCTCTGGCACTG
TGTCGTCCGCCCGCCACGCAGACTGGCGCGTCCAGGTGGCCGTGGCGCAC
CTGCACATCCACACTCCGCTGCTCGACAGTGAAAGAAAGGATGTCTTAAG
AGAAGCTGAAATTTTACACAAAGCTAGATTTAGTTACATTCTTCCAATTT
TGGGAATTTGCAATGAGCCTGAATTTTTGGGAATAGTTACTGAATACATG
CCAAATGGATCATTAAATGAACTCCTACATAGGAAAACTGAATATCCTGA
TGTTGCTTGGCCATTGAGATTTCGCATCCTGCATGAAATTGCCCTTGGTG
TAAATTACCTGCACAATATGACTCCTCCTTTACTTCATCATGACTTGAAG
ACTCAGAATATCTTATTGGACAATGAATTTCATGTTAAGATTGCAGATTT
TGGTTTATCAAAGTGGCGCATGATGTCCCTCTCACAGTCACGAAGTAGCA
AATCTGCACCAGAAGGAGGGACAATTATCTATATGCCACCTGAAAACTAT
GAACCTGGACAAAAATCAAGGGCCAGTATCAAGCACGATATATATAGCTA
TGCAGTTATCACATGGGAAGTGTTATCCAGAAAACAGCCTTTTGAAGATG
TCACCAATCCTTTGCAGATAATGTATAGTGTGTCACAAGGACATCGACCT
GTTATTAATGAAGAAAGTTTGCCATATGATATACCTCACCGAGCACGTAT
GATCTCTCTAATAGAAAGTGGATGGGCACAAAATCCAGATGAAAGACCAT
CTTTCTTAAAATGTTTAATAGAACTTGAACCAGTTTTGAGAACATTTGAA
GAGATAACTTTTCTTGAAGCTGTTATTCAGCTAAAGAAAACAAAGTTACA
GAGTGTTTCAAGTGCCATTCACCTATGTGACAAGAAGAAAATGGAATTAT
CTCTGAACATACCTGTAAATCATGGTCCACAAGAGGAATCATGTGGATCC
TCTCAGCTCCATGAAAATAGTGGTTCTCCTGAAACTTCAAGGTCCCTGCC
AGCTCCTCAAGACAATGATTTTTTATCTAGAAAAGCTCAAGACTGTTATT
TTATGAAGCTGCATCACTGTCCTGGAAATCACAGTTGGGATAGCACCATT
TCTGGATCTCAAAGGGCTGCATTCTGTGATCACAAGACCACTCCATGCTC
TTCAGCAATAATAAATCCACTCTCAACTGCAGGAAACTCAGAACGTCTGC
AGCCTGGTATAGCCCAGCAGTGGATCCAGAGCAAAAGGGAAGACATTGTG
AACCAAATGACAGAAGCCTGCCTTAACCAGTCGCTAGATGCCCTTCTGTC
CAGGGACTTGATCATGAAAGAGGACTATGAACTTGTTAGTACCAAGCCTA
CAAGGACCTCAAAAGTCAGACAATTACTAGACACTACTGACATCCAAGGA
GAAGAATTTGCCAAAGTTATAGTACAAAAATTGAAAGATAACAAACAAAT
GGGTCTTCAGCCTTACCCGGAAATACTTGTGGTTTCTAGATCACCATCTT
TAAATTTACTTCAAAATAAAAGCATGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMNGEAICSALPTIPYH
KLADLRYLSRGASGTVSSARHADWRVQVAVAHLHIHTPLLDSERKDVLRE
AEILHKARFSYILPILGICNEPEFLGIVTEYMPNGSLNELLHRKTEYPDV
AWPLRFRILHEIALGVNYLHNMTPPLLHHDLKTQNILLDNEFHVKIADFG
LSKWRMMSLSQSRSSKSAPEGGTIIYMPPENYEPGQKSRASIKHDIYSYA
VITWEVLSRKQPFEDVTNPLQIMYSVSQGHRPVINEESLPYDIPHRARMI
SLIESGWAQNPDERPSFLKCLIELEPVLRTFEEITFLEAVIQLKKTKLQS
VSSAIHLCDKKKMELSLNIPVNHGPQEESCGSSQLHENSGSPETSRSLPA
PQDNDFLSRKAQDCYFMKLHHCPGNHSWDSTISGSQRAAFCDHKTTPCSS
AIINPLSTAGNSERLQPGIAQQWIQSKREDIVNQMTEACLNQSLDALLSR
DLIMKEDYELVSTKPTRTSKVRQLLDTTDIQGEEFAKVIVQKLKDNKQMG
LQPYPEILVVSRSPSLNLLQNKSM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8747
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; N.B. sequence change compared with sequence NP_003812: t1200a (silent in G400). This change present in EST sequences.; ; Estimated MW of expressed protein : 88333 Daltons