cDNA Clone RIPK1

• Plasmid: pGEX6P-RIPK1 K377R
• Parent Plasmid: pGEX6P1
• Expressed: GST-RIPK1 K377R
• Genbank: NM_003804
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCCAACCAGACATGTCCTTGAATGTCATTAAGATGAAATCCAGTGA
CTTCCTGGAGAGTGCAGAACTGGACAGCGGAGGCTTCGGGAAGGTGTCTC
TGTGTTTCCACAGAACCCAGGGACTCATGATCATGAAAACAGTGTACAAG
GGGCCCAACTGCATTGAGCACAACGAGGCCCTCTTGGAGGAGGCGAAGAT
GATGAACAGACTGAGACACAGCCGGGTGGTGAAGCTCCTGGGCGTCATCA
TAGAGGAAGGGAAGTACTCCCTGGTGATGGAGTACATGGAGAAGGGCAAC
CTGATGCACGTGCTGAAAGCCGAGATGAGTACTCCGCTTTCTGTAAAAGG
AAGGATAATTTTGGAAATCATTGAAGGAATGTGCTACTTACATGGAAAAG
GCGTGATACACAAGGACCTGAAGCCTGAAAATATCCTTGTTGATAATGAC
TTCCACATTAAGATCGCAGACCTCGGCCTTGCCTCCTTTAAGATGTGGAG
CAAACTGAATAATGAAGAGCACAATGAGCTGAGGGAAGTGGACGGCACCG
CTAAGAAGAATGGCGGCACCCTCTACTACATGGCGCCCGAGCACCTGAAT
GACGTCAACGCAAAGCCCACAGAGAAGTCGGATGTGTACAGCTTTGCTGT
AGTACTCTGGGCGATATTTGCAAATAAGGAGCCATATGAAAATGCTATCT
GTGAGCAGCAGTTGATAATGTGCATAAAATCTGGGAACAGGCCAGATGTG
GATGACATCACTGAGTACTGCCCAAGAGAAATTATCAGTCTCATGAAGCT
CTGCTGGGAAGCGAATCCGGAAGCTCGGCCGACATTTCCTGGCATTGAAG
AAAAATTTAGGCCTTTTTATTTAAGTCAATTAGAAGAAAGTGTAGAAGAG
GACGTGAAGAGTTTAAAGAAAGAGTATTCAAACGAAAATGCAGTTGTGAA
GAGAATGCAGTCTCTTCAACTTGATTGTGTGGCAGTACCTTCAAGCCGGT
CAAATTCAGCCACAGAACAGCCTGGTTCACTGCACAGTTCCCAGGGACTT
GGGATGGGTCCTGTGGAGGAGTCCTGGTTTGCTCCTTCCCTGGAGCACCC
ACAAGAAGAGAATGAGCCCAGCCTGCAGAGTAGACTCCAAGACGAAGCCA
ACTACCATCTTTATGGCAGCCGCATGGACAGGCAGACGAAACAGCAGCCC
AGACAGAATGTGGCTTACAACAGAGAGGAGGAAAGGAGACGCAGGGTCTC
CCATGACCCTTTTGCACAGCAAAGACCTTACGAGAATTTTCAGAATACAG
AGGGAAAAGGCACTGCTTATTCCAGTGCAGCCAGTCATGGTAATGCAGTG
CACCAGCCCTCAGGGCTCACCAGCCAACCTCAAGTACTGTATCAGAACAA
TGGATTATATAGCTCACATGGCTTTGGAACAAGACCACTGGATCCAGGAA
CAGCAGGTCCCAGAGTTTGGTACAGGCCAATTCCAAGTCATATGCCTAGT
CTGCATAATATCCCAGTGCCTGAGACCAACTATCTAGGAAATACACCCAC
CATGCCATTCAGCTCCTTGCCACCAACAGATGAATCTATAAAATATACCA
TATACAATAGTACTGGCATTCAGATTGGAGCCTACAATTATATGGAGATT
GGTGGGACGAGTTCATCACTACTAGACAGCACAAATACGAACTTCAAAGA
AGAGCCAGCTGCTAAGTACCAAGCTATCTTTGATAATACCACTAGTCTGA
CGGATAAACACCTGGACCCAATCAGGGAAAATCTGGGAAAGCACTGGAAA
AACTGTGCCCGTAAACTGGGCTTCACACAGTCTCAGATTGATGAAATTGA
CCATGACTATGAGCGAGATGGACTGAAAGAAAAGGTTTACCAGATGCTCC
AAAAGTGGGTGATGAGGGAAGGCATAAAGGGAGCCACGGTGGGGAAGCTG
GCCCAGGCGCTCCACCAGTGTTCCAGGATCGACCTTCTGAGCAGCTTGAT
TTACGTCAGCCAGAACTAAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFQPDMSLNVIKMKSSDF
LESAELDSGGFGKVSLCFHRTQGLMIMKTVYKGPNCIEHNEALLEEAKMM
NRLRHSRVVKLLGVIIEEGKYSLVMEYMEKGNLMHVLKAEMSTPLSVKGR
IILEIIEGMCYLHGKGVIHKDLKPENILVDNDFHIKIADLGLASFKMWSK
LNNEEHNELREVDGTAKKNGGTLYYMAPEHLNDVNAKPTEKSDVYSFAVV
LWAIFANKEPYENAICEQQLIMCIKSGNRPDVDDITEYCPREIISLMKLC
WEANPEARPTFPGIEEKFRPFYLSQLEESVEEDVKSLKKEYSNENAVVKR
MQSLQLDCVAVPSSRSNSATEQPGSLHSSQGLGMGPVEESWFAPSLEHPQ
EENEPSLQSRLQDEANYHLYGSRMDRQTKQQPRQNVAYNREEERRRRVSH
DPFAQQRPYENFQNTEGKGTAYSSAASHGNAVHQPSGLTSQPQVLYQNNG
LYSSHGFGTRPLDPGTAGPRVWYRPIPSHMPSLHNIPVPETNYLGNTPTM
PFSSLPPTDESIKYTIYNSTGIQIGAYNYMEIGGTSSSLLDSTNTNFKEE
PAAKYQAIFDNTTSLTDKHLDPIRENLGKHWKNCARKLGFTQSQIDEIDH
DYERDGLKEKVYQMLQKWVMREGIKGATVGKLAQALHQCSRIDLLSSLIY
VSQN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8714
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; N.B. Sequence differences compared with NM_003804 CDS:; t84c (silent in F28); t1313c (V438A). ; Both present in other published mRNAs for RIPK1.; ; Estimated MW of expressed protein : 103022 Daltons