cDNA Clone RIPK1

• Plasmid: pEBG6P-RIPK1 K377R
• Parent Plasmid: pEBG6P
• Expressed: GST-RIPK1 K377R
• Genbank: NM_003804
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGCAACCAGACATGTCCTTGAATGTCATTAAGATGAAATCCAG
TGACTTCCTGGAGAGTGCAGAACTGGACAGCGGAGGCTTCGGGAAGGTGT
CTCTGTGTTTCCACAGAACCCAGGGACTCATGATCATGAAAACAGTGTAC
AAGGGGCCCAACTGCATTGAGCACAACGAGGCCCTCTTGGAGGAGGCGAA
GATGATGAACAGACTGAGACACAGCCGGGTGGTGAAGCTCCTGGGCGTCA
TCATAGAGGAAGGGAAGTACTCCCTGGTGATGGAGTACATGGAGAAGGGC
AACCTGATGCACGTGCTGAAAGCCGAGATGAGTACTCCGCTTTCTGTAAA
AGGAAGGATAATTTTGGAAATCATTGAAGGAATGTGCTACTTACATGGAA
AAGGCGTGATACACAAGGACCTGAAGCCTGAAAATATCCTTGTTGATAAT
GACTTCCACATTAAGATCGCAGACCTCGGCCTTGCCTCCTTTAAGATGTG
GAGCAAACTGAATAATGAAGAGCACAATGAGCTGAGGGAAGTGGACGGCA
CCGCTAAGAAGAATGGCGGCACCCTCTACTACATGGCGCCCGAGCACCTG
AATGACGTCAACGCAAAGCCCACAGAGAAGTCGGATGTGTACAGCTTTGC
TGTAGTACTCTGGGCGATATTTGCAAATAAGGAGCCATATGAAAATGCTA
TCTGTGAGCAGCAGTTGATAATGTGCATAAAATCTGGGAACAGGCCAGAT
GTGGATGACATCACTGAGTACTGCCCAAGAGAAATTATCAGTCTCATGAA
GCTCTGCTGGGAAGCGAATCCGGAAGCTCGGCCGACATTTCCTGGCATTG
AAGAAAAATTTAGGCCTTTTTATTTAAGTCAATTAGAAGAAAGTGTAGAA
GAGGACGTGAAGAGTTTAAAGAAAGAGTATTCAAACGAAAATGCAGTTGT
GAAGAGAATGCAGTCTCTTCAACTTGATTGTGTGGCAGTACCTTCAAGCC
GGTCAAATTCAGCCACAGAACAGCCTGGTTCACTGCACAGTTCCCAGGGA
CTTGGGATGGGTCCTGTGGAGGAGTCCTGGTTTGCTCCTTCCCTGGAGCA
CCCACAAGAAGAGAATGAGCCCAGCCTGCAGAGTAGACTCCAAGACGAAG
CCAACTACCATCTTTATGGCAGCCGCATGGACAGGCAGACGAAACAGCAG
CCCAGACAGAATGTGGCTTACAACAGAGAGGAGGAAAGGAGACGCAGGGT
CTCCCATGACCCTTTTGCACAGCAAAGACCTTACGAGAATTTTCAGAATA
CAGAGGGAAAAGGCACTGCTTATTCCAGTGCAGCCAGTCATGGTAATGCA
GTGCACCAGCCCTCAGGGCTCACCAGCCAACCTCAAGTACTGTATCAGAA
CAATGGATTATATAGCTCACATGGCTTTGGAACAAGACCACTGGATCCAG
GAACAGCAGGTCCCAGAGTTTGGTACAGGCCAATTCCAAGTCATATGCCT
AGTCTGCATAATATCCCAGTGCCTGAGACCAACTATCTAGGAAATACACC
CACCATGCCATTCAGCTCCTTGCCACCAACAGATGAATCTATAAAATATA
CCATATACAATAGTACTGGCATTCAGATTGGAGCCTACAATTATATGGAG
ATTGGTGGGACGAGTTCATCACTACTAGACAGCACAAATACGAACTTCAA
AGAAGAGCCAGCTGCTAAGTACCAAGCTATCTTTGATAATACCACTAGTC
TGACGGATAAACACCTGGACCCAATCAGGGAAAATCTGGGAAAGCACTGG
AAAAACTGTGCCCGTAAACTGGGCTTCACACAGTCTCAGATTGATGAAAT
TGACCATGACTATGAGCGAGATGGACTGAAAGAAAAGGTTTACCAGATGC
TCCAAAAGTGGGTGATGAGGGAAGGCATAAAGGGAGCCACGGTGGGGAAG
CTGGCCCAGGCGCTCCACCAGTGTTCCAGGATCGACCTTCTGAGCAGCTT
GATTTACGTCAGCCAGAACTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAQPDM
SLNVIKMKSSDFLESAELDSGGFGKVSLCFHRTQGLMIMKTVYKGPNCIE
HNEALLEEAKMMNRLRHSRVVKLLGVIIEEGKYSLVMEYMEKGNLMHVLK
AEMSTPLSVKGRIILEIIEGMCYLHGKGVIHKDLKPENILVDNDFHIKIA
DLGLASFKMWSKLNNEEHNELREVDGTAKKNGGTLYYMAPEHLNDVNAKP
TEKSDVYSFAVVLWAIFANKEPYENAICEQQLIMCIKSGNRPDVDDITEY
CPREIISLMKLCWEANPEARPTFPGIEEKFRPFYLSQLEESVEEDVKSLK
KEYSNENAVVKRMQSLQLDCVAVPSSRSNSATEQPGSLHSSQGLGMGPVE
ESWFAPSLEHPQEENEPSLQSRLQDEANYHLYGSRMDRQTKQQPRQNVAY
NREEERRRRVSHDPFAQQRPYENFQNTEGKGTAYSSAASHGNAVHQPSGL
TSQPQVLYQNNGLYSSHGFGTRPLDPGTAGPRVWYRPIPSHMPSLHNIPV
PETNYLGNTPTMPFSSLPPTDESIKYTIYNSTGIQIGAYNYMEIGGTSSS
LLDSTNTNFKEEPAAKYQAIFDNTTSLTDKHLDPIRENLGKHWKNCARKL
GFTQSQIDEIDHDYERDGLKEKVYQMLQKWVMREGIKGATVGKLAQALHQ
CSRIDLLSSLIYVSQN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8713
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express with N-terminal GST (contains precission protease site); N.B. Sequence differences compared with NM_003804 CDS:; t84c (silent in F28); t1313c (V438A). ; Both present in other published mRNAs for RIPK1.; ; Estimated MW of expressed protein : 104046 Daltons