cDNA Clone RIPK5

• Plasmid: pGEX6P-1-RIPK5
• Parent Plasmid: pGEX6P1
• Expressed: GST-RIPK5
• Genbank: AAH72406
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAGGGCGACGGGGTGCCATGGGGCAGCGAGCCCGTCTCGGG
TCCCGGCCCCGGCGGCGGCGGAATGATCCGCGAGCTGTGCCGGGGCTTCG
GCCGCTACCGCCGCTACCTGGGACGGCTGCGACAGAACCTGCGCGAGACC
CAGAAGTTCTTCCGCGACATCAAGTGCTCCCACAACCACACTTGTCTCTC
CTCCCTCACGGGCGGCGGCGGGGCCGAGCGCGGCCCTGCAGGCGATGTCG
CCGAAACCGGGCTGCAGGCGGGCCAACTGAGCTGCATTTCCTTCCCACCT
AAGGAAGAGAAGTACCTCCAGCAGATTGTGGACTGCCTCCCTTGCATACT
GATCCTCGGCCAGGATTGTAACGTCAAGTGCCAGCTGTTGAATCTGCTGT
TGGGGGTGCAGGTGCTTCCCACCACCAAGCTGGGCAGTGAGGAGAGCTGT
AAGCTTCGGCGCCTCCGCTTCACCTATGGGACTCAGACTCGGGTCAGCCT
GGCGCTCCCTGGACAGTATGAACTAGTGCACACGCTGGTTGCTCATCAGG
GCAACTGGGAGACCATCCCTGAGGAGGATCTGGAGGTCCAAGAGAACAAT
GAGGATGCTGCTCATGTTTTAGCGGAACTGGAGGTAACGATGCACCATGC
TCTCTTACAGGAAGTGGACGTTGTGGTAGCACCATGCCAAGGCCTCCGGC
CCACAGTGGATGTTCTGGGTGACTTGGTGAATGATTTCTTGCCTGCGATA
ACCTATGCACTCCACAAAGATGAACTCTCTGAGAGGGATGAGCAAGAGCT
TCAGGAAATCCGAAAGTATTTCTCCTTTCCTGTATTCTTTTTCAAAGTGC
CGAAACTGGGCTCGGAGATAATAGACTCCTCAACCAGGAGAATGGAGAGC
GAAAGATCACCGCTTTATCGCCAGCTAATTGACCTGGGCTATCTGAGCAG
CAGTCACTGGAACTGTGGGGCTCCTGGCCAGGATACTAAAGCTCAGAGCA
TGTTGGTGGAACAGAGTGAAAAGCTGAGACACTTGAGCACATTTTCTCAC
CAGGTGTTACAGACTCGCCTGGTGGATGCAGCCAAGGCCCTGAACCTGGT
GCACTGCCACTGCCTTGACATCTTTATTAACCAGGCATTTGACATGCAGC
GGGACCTGCAGATCACTCCCAAACGTCTGGAATATACTCGAAAAAAGGAG
AATGAGTTGTATGAATCATTGATGAATATTGCCAACCGAAAGCAGGAGGA
AATGAAGGATATGATTGTTGAGACACTTAATACCATGAAGGAGGAACTTC
TGGATGATGCTACTAACATGGAGTTTAAAGACGTCATTGTCCCTGAGAAT
GGAGAACCAGTAGGCACCAGAGAGATCAAATGCTGCATCCGACAGATCCA
GGAACTCATCATCTCCCGACTTAATCAGGCAGTGGCTAATAAGCTGATCA
GCTCAGTGGATTACCTGAGGGAAAGCTTCGTCGGAACCCTGGAACGATGT
CTGCAGAGCCTGGAGAAGTCTCAGGATGTCTCAGTTCACATCACCAGTAA
TTATCTCAAACAGATCTTAAATGCTGCCTATCATGTTGAAGTCACGTTTC
ACTCAGGGTCGTCAGTTACAAGGATGCTATGGGAGCAAATCAAACAGATC
ATCCAGCGCATCACATGGGTGAGCCCACCTGCCATCACTCTGGAATGGAA
GAGGAAGGTGGCCCAGGAAGCCATTGAGAGCCTCAGCGCCTCCAAATTGG
CTAAGAGCATTTGCAGCCAATTCCGGACTCGGCTCAATAGTTCCCACGAG
GCTTTTGCAGCCTCCTTGCGGCAGCTGGAAGCTGGCCACTCAGGCCGGTT
AGAGAAAACGGAAGATCTATGGCTGAGGGTTCGGAAAGATCATGCTCCCC
GCCTGGCCCGCCTTTCTCTGGAAAGCCGTTCTTTACAGGATGTCTTGCTT
CATCGTAAACCTAAACTGGGACAGGAACTGGGCCGGGGCCAGTATGGTGT
GGTATACCTGTGTGACAACTGGGGAGGACACTTTCCTTGTGCCCTCAAAT
CAGTTGTCCCTCCAGATGAGAAGCACTGGAATGATCTGGCTTTGGAATTT
CACTATATGAGGTCTCTGCCGAAGCATGAGCGATTGGTGGATCTCCATGG
TTCAGTCATTGACTACAACTATGGTGGTGGCTCCAGCATTGCTGTGCTCC
TCATTATGGAGCGGCTACACCGGGATCTCTACACAGGGCTGAAGGCTGGG
CTGACCCTGGAGACACGTTTGCAGATAGCACTAGATGTGGTGGAGGGAAT
CCGCTTCCTGCACAGCCAGGGACTTGTCCATCGTGATATCAAACTGAAAA
ATGTGCTGCTGGATAAGCAGAACCGTGCCAAGATCACTGACTTAGGATTC
TGCAAGCCAGAGGCCATGATGTCAGGCAGCATTGTGGGGACACCAATCCA
TATGGCCCCTGAACTTTTCACAGGGAAGTACGATAATTCCGTGGATGTCT
ACGCTTTTGGAATTCTTTTCTGGTATATCTGCTCAGGCTCTGTCAAGCTC
CCTGAGGCATTTGAGAGGTGTGCTAGCAAAGACCATCTCTGGAACAATGT
GCGGAGGGGGGCTCGCCCAGAACGTCTTCCTGTGTTTGATGAGGAGTGCT
GGCAGTTGATGGAAGCCTGTTGGGATGGCGACCCCTTGAAGAGGCCTCTC
TTGGGCATTGTCCAGCCCATGCTCCAGGGCATCATGAATCGGCTCTGCAA
GTCCAATTCTGAGCAGCCAAACAGAGGACTAGATGATTCTACTTGAGCGG
CCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEGDGVPWGSEPVSGPGPG
GGGMIRELCRGFGRYRRYLGRLRQNLRETQKFFRDIKCSHNHTCLSSLTG
GGGAERGPAGDVAETGLQAGQLSCISFPPKEEKYLQQIVDCLPCILILGQ
DCNVKCQLLNLLLGVQVLPTTKLGSEESCKLRRLRFTYGTQTRVSLALPG
QYELVHTLVAHQGNWETIPEEDLEVQENNEDAAHVLAELEVTMHHALLQE
VDVVVAPCQGLRPTVDVLGDLVNDFLPAITYALHKDELSERDEQELQEIR
KYFSFPVFFFKVPKLGSEIIDSSTRRMESERSPLYRQLIDLGYLSSSHWN
CGAPGQDTKAQSMLVEQSEKLRHLSTFSHQVLQTRLVDAAKALNLVHCHC
LDIFINQAFDMQRDLQITPKRLEYTRKKENELYESLMNIANRKQEEMKDM
IVETLNTMKEELLDDATNMEFKDVIVPENGEPVGTREIKCCIRQIQELII
SRLNQAVANKLISSVDYLRESFVGTLERCLQSLEKSQDVSVHITSNYLKQ
ILNAAYHVEVTFHSGSSVTRMLWEQIKQIIQRITWVSPPAITLEWKRKVA
QEAIESLSASKLAKSICSQFRTRLNSSHEAFAASLRQLEAGHSGRLEKTE
DLWLRVRKDHAPRLARLSLESRSLQDVLLHRKPKLGQELGRGQYGVVYLC
DNWGGHFPCALKSVVPPDEKHWNDLALEFHYMRSLPKHERLVDLHGSVID
YNYGGGSSIAVLLIMERLHRDLYTGLKAGLTLETRLQIALDVVEGIRFLH
SQGLVHRDIKLKNVLLDKQNRAKITDLGFCKPEAMMSGSIVGTPIHMAPE
LFTGKYDNSVDVYAFGILFWYICSGSVKLPEAFERCASKDHLWNNVRRGA
RPERLPVFDEECWQLMEACWDGDPLKRPLLGIVQPMLQGIMNRLCKSNSE
QPNRGLDDST*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8682
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression. ; ; Estimated MW of expressed protein : 132051 Daltons