cDNA Clone VPS15

• Plasmid: pFB Dual 6His VPS34 mTOR VPS15
• Parent Plasmid: pFastbacDual
• Expressed: 6His VPS34 mTOR VPS15
• Genbank: NP_002638 and NM_014602
• Tag: 6His
• Species: Human

Sequence of Insert

DNA sequence of VPS34 insert : GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGGGGAAGC
AGAGAAGTTTCACTACATCTATAGTTGTGACCTGGATATCAACGTCCAGC
TTAAGATAGGAAGCTTGGGAGGGAAGAGAGAACAAAAGAGTTATAAAGCT
GTCCTGGAAGACCCAATGTTGAAGTTCTCAGGACTATATCAAGAGACATG
CTCTGATCTTTATGTTACTTGTCAAGTTTTTGCAGAAGGGAAGCCTTTGG
CCTTGCCAGTGAGAACATCCTACAAAGCATTTAGTACAAGATGGAACTGG
AATGAATGGCTGAAACTACCAGTAAAATACCCTGACCTGCCCAGGAATGC
CCAAGTGGCCCTCACCATATGGGATGTGTATGGTCCCGGAAAAGCAGTGC
CTGTAGGAGGAACAACGGTTTCGCTCTTTGGAAAATACGGCATGTTTCGC
CAAGGGATGCATGACTTGAAAGTCTGGCCTAATGTAGAAGCAGATGGATC
AGAACCCACAAAAACTCCTGGCAGAACAAGTAGCACTCTCTCAGAAGATC
AGATGAGCCGTCTTGCCAAGCTCACCAAAGCTCATCGACAAGGACACATG
GTGAAAGTAGATTGGCTGGATAGATTGACATTTAGAGAAATAGAAATGAT
AAATGAGAGTGAAAAACGAAGTTCTAATTTCATGTACCTGATGGTTGAAT
TTCGATGTGTCAAGTGTGATGATAAGGAATATGGTATTGTTTATTATGAA
AAGGACGGTGATGAATCATCTCCAATTTTAACAAGTTTTGAATTAGTGAA
AGTTCCTGACCCCCAGATGTCTATGGAGAATTTAGTTGAGAGCAAACACC
ACAAGCTTGCCCGGAGTTTAAGAAGTGGACCTTCTGACCACGATCTGAAA
CCCAATGCTGCCACGAGAGATCAGTTAAATATTATTGTGAGTTATCCACC
AACCAAGCAACTTACATATGAAGAACAAGATCTTGTTTGGAAGTTTAGAT
ATTATCTTACGAATCAAGAAAAAGCCTTGACAAAATTCTTGAGATGTGTT
AATTGGGATCTACCTCAAGAGGCCAAACAGGCCTTGGAACTTCTGGGAAA
ATGGAAGCCGATGGATGTAGAGGACTCCTTGGAGCTGTTATCCTCTCATT
ACACCAACCCAACTGTGAGGCGCTATGCTGTTGCCCGGTTGCGACAGGCC
GATGATGAGGATTTGTTGATGTACCTATTACAATTGGTCCAGGCTCTCAA
ATATGAAAATTTTGATGATATAAAGAATGGATTGGAACCTACCAAGAAGG
ATAGTCAGAGTTCAGTGTCAGAAAATGTGTCAAATTCTGGAATAAATTCT
GCAGAAATAGATAGCTCCCAAATTATAACCAGCCCCCTTCCTTCAGTCTC
TTCACCTCCTCCTGCATCAAAAACAAAAGAAGTTCCAGATGGCGAAAATC
TGGAACAAGATCTCTGTACCTTCTTGATATCGAGAGCCTGCAAAAACTCA
ACACTGGCTAATTATTTATACTGGTATGTGATAGTGGAATGTGAAGATCA
AGATACTCAGCAGAGAGATCCAAAGACCCATGAGATGTACTTGAACGTAA
TGAGAAGATTCAGCCAAGCATTGTTGAAGGGTGATAAGTCTGTCAGAGTT
ATGCGTTCTTTGCTGGCTGCACAACAGACATTTGTAGATCGGTTGGTGCA
TCTAATGAAGGCAGTACAACGCGAAAGTGGAAATCGTAAGAAAAAGAATG
AGAGACTACAGGCATTGCTTGGAGATAATGAAAAGATGAATTTGTCAGAT
GTGGAACTTATCCCGTTGCCTTTAGAACCCCAAGTGAAAATTAGAGGAAT
AATTCCGGAAACAGCTACACTGTTTAAAAGTGCCCTTATGCCTGCACAGT
TGTTTTTTAAGACGGAAGATGGAGGCAAATATCCAGTTATATTTAAGCAT
GGAGATGATTTACGTCAAGATCAACTTATTCTTCAAATCATTTCACTCAT
GGACAAGCTGTTACGGAAAGAAAATCTGGACTTGAAATTGACACCTTATA
AGGTGTTAGCCACCAGTACAAAACATGGCTTCATGCAGTTTATCCAGTCA
GTTCCTGTGGCTGAAGTTCTTGATACAGAGGGAAGCATTCAGAACTTTTT
TAGAAAATATGCACCAAGTGAGAATGGGCCAAATGGGATTAGTGCTGAGG
TCATGGACACTTACGTTAAAAGCTGTGCTGGATATTGCGTGATCACCTAT
ATACTTGGAGTTGGAGACAGGCACCTGGATAACCTTTTGCTAACAAAAAC
AGGCAAACTCTTCCACATAGACTTTGGATATATTTTGGGTCGGGCTATGA
CCCGAGAGAAGTTTCCAGAGAAGATTCCACCAATGAAGCTGAATAAAGAA
ATGGTAGAAGGAATGGGGGGCACACAGAGTGAGCAGTACCAAGAGTTCCG
TAAACAGTGTTACACGGCTTTCCTCCACCTGCGAAGGTATTCTAATCTGA
TTTTGAACTTGTTTTCCTTGATGGTTGATGCAAACATTCCAGATATTGCA
CTTGAACCAGATAAAACTGTGAAAAAGGTTCAGGATAAATTCCGCTTAGA
CCTGTCGGATGAAGAGGCTGTGCATTACATGCAGAGTCTGATTGATGAGA
GTGTCCATGCTCTTTTTGCTGCAGTGGTGGAACAGATTCACAAGTTTGCC
CAGTACTGGAGAAAATGAGAATTC

DNA sequence of VPS15 insert : GTCGACGCCACCATGGGAAATCAGCTTGCTGGCATTGCTCCCTCCCAGAT
CCTTTCTGTAGAGAGTTATTTTTCAGATATTCATGACTTTGAATATGATA
AAAGCCTGGGGAGTACTCGGTTTTTTAAAGTTGCTCGAGCCAAGCACCGA
GAAGGCCTGGTCGTTGTGAAGGTTTTTGCAATTCAGGATCCCACATTGCC
TTTAACCAGCTATAAACAAGAGCTGGAGGAACTGAAAATCAGGCTTAATT
CTGCACAGAATTGTCTACCTTTCCAGAAAGCATCAGAAAAAGCATCTGAG
AAAGCAGCTATGCTCTTTAGGCAGTATGTGCGAGACAATCTCTATGATCG
CATCAGTACCCGTCCATTCTTGAATAACATTGAGAAGCGCTGGATTGCTT
TCCAGATCCTGACAGCTGTGGACCAAGCACACAAATCTGGAGTTCGTCAT
GGGGACATCAAGACTGAGAATGTGATGGTCACCAGTTGGAATTGGGTTCT
TCTAACTGATTTTGCCAGTTTTAAGCCCACTTATCTTCCAGAAGACAACC
CGGCAGATTTCAATTATTTCTTTGACACATCACGGAGGAGAACTTGCTAT
ATTGCTCCTGAACGTTTTGTTGATGGTGGGATGTTTGCCACTGAGTTAGA
ATATATGAGAGATCCTTCAACTCCGCTTGTAGACTTAAATAGCAATCAGA
GAACAAGAGGAGAGTTGAAGAGAGCAATGGACATCTTTTCAGCAGGTTGT
GTGATAGCTGAGCTTTTTACAGAAGGTGTACCATTATTTGATCTCTCTCA
ACTTTTGGCTTATAGAAATGGACATTTTTTCCCTGAACAAGTGCTAAATA
AAATTGAAGATCACAGTATCAGAGAATTGGTAACTCAGATGATTCACCGT
GAGCCAGATAAACGTTTAGAGGCAGAAGATTACTTAAAACAGCAGCGTGG
CAATGCCTTTCCTGAAATATTTTACACTTTTCTTCAGCCCTACATGGCCC
AGTTTGCCAAGGAAACGTTTCTTTCTGCAGATGAGCGTATTCTGGTTATA
CGGAAGGATTTGGGCAACATTATTCACAATCTCTGTGGACATGATCTGCC
AGAAAAAGCCGAAGGAGAGCCTAAGGAAAATGGGCTGGTTATCTTGGTAT
CTGTTATAACATCCTGCCTACAGACCCTTAAATACTGTGATTCCAAACTA
GCTGCTTTGGAACTGATTCTTCATTTGGCTCCAAGATTAAGTGTTGAAAT
CCTTTTGGATCGTATTACTCCATATCTTTTGCATTTCAGCAATGACTCTG
TTCCTAGGGTGAGGGCTGAAGCCTTGAGGACGTTGACCAAAGTTCTTGCT
CTCGTCAAAGAGGTTCCTCGTAATGATATCAATATTTATCCGGAATACAT
TCTGCCAGGCATAGCCCACTTAGCCCAAGATGATGCTACTATCGTTAGAC
TAGCCTATGCTGAAAACATAGCTCTGCTGGCAGAAACAGCTCTGAGATTC
CTGGAATTAGTACAGTTAAAAAATCTTAATATGGAAAATGACCCCAATAA
TGAAGAAATAGATGAGGTTACACATCCAAATGGAAATTATGACACAGAGC
TCCAAGCCTTACATGAAATGGTCCAGCAGAAAGTTGTTACTTTGCTAAGT
GACCCTGAAAATATTGTAAAACAAACCTTGATGGAAAATGGAATAACACG
GCTGTGTGTATTCTTTGGACGTCAGAAAGCCAACGATGTTTTGTTGTCCC
ACATGATTACTTTCCTAAATGATAAGAATGATTGGCATCTACGTGGAGCA
TTTTTTGATAGTATAGTTGGTGTTGCTGCCTATGTTGGCTGGCAAAGCTC
CTCAATTCTCAAGCCTCTGCTGCAACAAGGTCTTAGTGATGCTGAGGAAT
TTGTCATTGTGAAAGCTCTTTATGCCCTTACTTGTATGTGCCAGTTAGGA
CTGCTACAAAAACCCCATGTTTACGAATTTGCCAGTGATATTGCCCCCTT
CCTGTGTCATCCCAATTTATGGATACGTTATGGTGCCGTGGGATTTATCA
CAGTGGTAGCTCGTCAAATAAGTACAGCTGATGTCTACTGTAAACTGATG
CCTTATCTTGACCCATATATTACCCAACCAATAATACAGATTGAAAGAAA
ACTTGTTCTGCTCAGTGTTTTAAAGGAACCAGTAAGTCGTTCTATATTTG
ATTATGCTTTGAGGTCTAAAGATATTACTAGCTTGTTCAGACATCTTCAC
ATGCGTCAGAAGAAACGAAATGGTTCTCTTCCCGACTGCCCTCCGCCAGA
GGATCCTGCCATAGCACAGCTTCTGAAGAAGTTGCTCTCACAGGGAATGA
CAGAGGAAGAGGAAGACAAACTTCTGGCACTGAAAGACTTCATGATGAAA
TCTAATAAAGCAAAGGCCAATATAGTGGACCAGAGCCATCTTCATGATAG
TAGTCAGAAAGGTGTAATTGACTTGGCAGCTTTAGGCATAACTGGGAGAC
AAGTTGATCTTGTTAAAACCAAACAAGAACCAGATGACAAACGGGCCAGA
AAACATGTAAAACAAGACTCAAATGTAAATGAAGAATGGAAAAGCATGTT
TGGGTCACTGGACCCACCAAACATGCCACAGGCCCTACCTAAAGGGAGTG
ATCAGGAGGTGATTCAGACTGGGAAACCTCCTCGTTCCGAGTCCTCTGCT
GGCATTTGTGTCCCTTTGTCAACTTCTTCACAGGTTCCAGAAGTGACAAC
TGTCCAAAATAAAAAACCAGTAATACCGGTTTTAAGTAGTACAATCTTAC
CATCCACCTATCAGATTCGAATTACAACTTGTAAAACTGAACTTCAGCAA
CTCATCCAGCAAAAGCGGGAGCAGTGCAATGCTGAGAGAATAGCTAAGCA
GATGATGGAAAATGCTGAATGGGAGAGTAAACCACCACCACCTGGATGGC
GTCCTAAAGGGCTGTTAGTTGCCCATCTTCATGAGCATAAATCTGCTGTG
AATCGAATTAGAGTCTCTGATGAACACTCACTTTTTGCAACATGTTCAAA
TGATGGCACAGTGAAAATCTGGAACAGTCAAAAGATGGAGGGGAAGACCA
CCACTACCAGATCTATTCTTACATACAGCCGAATTGGAGGACGAGTCAAG
ACGCTCACATTCTGCCAAGGCTCCCACTATTTAGCCATAGCATCTGATAA
TGGTGCTGTCCAGCTTCTTGGAATTGAGGCTTCTAAGCTGCCCAAGTCTC
CTAAAATCCATCCTCTACAAAGCAGAATTCTAGATCAGAAGGAGGACGGT
TGTGTTGTGGATATGCATCACTTCAACTCTGGAGCACAGTCTGTTCTTGC
CTATGCCACTGTGAATGGCTCTCTGGTTGGCTGGGACCTTAGGTCTTCAA
GCAATGCGTGGACTTTAAAGCATGATTTAAAGTCGGGCCTCATCACTTCC
TTTGCTGTGGACATCCACCAATGCTGGCTCTGCATTGGTACAAGCAGTGG
TACCATGGCTTGTTGGGACATGAGGTTCCAGTTGCCAATTTCAAGTCACT
GTCATCCTTCCAGGGCTCGAATCAGACGCCTCTCAATGCACCCTCTGTAT
CAGTCCTGGGTGATTGCAGCTGTTCAGGGCAACAACGAAGTGTCCATGTG
GGACATGGAGACTGGTGACAGAAGATTTACTCTCTGGGCCAGCAGTGCAC
CACCACTTTCTGAATTACAGCCTTCTCCTCATAGCGTCCATGGTATCTAC
TGTAGTCCTGCAGATGGAAATCCTATCCTACTAACAGCTGGCTCAGATAT
GAAAATAAGGTTTTGGGACTTGGCTTACCCAGAAAGGTCCTATGTTGTTG
CAGGAAGTACTAGTTCCCCATCTGTGTCCTACTACAGGAAAATAATTGAA
GGCACTGAAGTTGTCCAGGAAATTCAGAATAAGCAGAAAGTAGGACCAAG
TGATGACACCCCTCGAAGGGGCCCAGAGTCCCTGCCCGTGGGACATCATG
ACATCATCACTGATGTCGCCACATTCCAGACCACACAGGGCTTCATCGTA
ACTGCTTCTAGAGATGGGATTGTGAAGGTGTGGAAATAAGCTAGCG

Amino Acid Sequence

Protein sequence of expressed VPS34 protein :
MSYYHHHHHHDYDIPTTENLYFQGAMDPEFATMYPYDVPDYAGEAEKFHY
IYSCDLDINVQLKIGSLGGKREQKSYKAVLEDPMLKFSGLYQETCSDLYV
TCQVFAEGKPLALPVRTSYKAFSTRWNWNEWLKLPVKYPDLPRNAQVALT
IWDVYGPGKAVPVGGTTVSLFGKYGMFRQGMHDLKVWPNVEADGSEPTKT
PGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESEK
RSSNFMYLMVEFRCVKCDDKEYGIVYYEKDGDESSPILTSFELVKVPDPQ
MSMENLVESKHHKLARSLRSGPSDHDLKPNAATRDQLNIIVSYPPTKQLT
YEEQDLVWKFRYYLTNQEKALTKFLRCVNWDLPQEAKQALELLGKWKPMD
VEDSLELLSSHYTNPTVRRYAVARLRQADDEDLLMYLLQLVQALKYENFD
DIKNGLEPTKKDSQSSVSENVSNSGINSAEIDSSQIITSPLPSVSSPPPA
SKTKEVPDGENLEQDLCTFLISRACKNSTLANYLYWYVIVECEDQDTQQR
DPKTHEMYLNVMRRFSQALLKGDKSVRVMRSLLAAQQTFVDRLVHLMKAV
QRESGNRKKKNERLQALLGDNEKMNLSDVELIPLPLEPQVKIRGIIPETA
TLFKSALMPAQLFFKTEDGGKYPVIFKHGDDLRQDQLILQIISLMDKLLR
KENLDLKLTPYKVLATSTKHGFMQFIQSVPVAEVLDTEGSIQNFFRKYAP
SENGPNGISAEVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKTGKLFH
IDFGYILGRAMTREKFPEKIPPMKLNKEMVEGMGGTQSEQYQEFRKQCYT
AFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEE
AVHYMQSLIDESVHALFAAVVEQIHKFAQYWRK*

Protein sequence of expressed VPS15 protein :
MGNQLAGIAPSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAKHREGLV
VVKVFAIQDPTLPLTSYKQELEELKIRLNSAQNCLPFQKASEKASEKAAM
LFRQYVRDNLYDRISTRPFLNNIEKRWIAFQILTAVDQAHKSGVRHGDIK
TENVMVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPE
RFVDGGMFATELEYMRDPSTPLVDLNSNQRTRGELKRAMDIFSAGCVIAE
LFTEGVPLFDLSQLLAYRNGHFFPEQVLNKIEDHSIRELVTQMIHREPDK
RLEAEDYLKQQRGNAFPEIFYTFLQPYMAQFAKETFLSADERILVIRKDL
GNIIHNLCGHDLPEKAEGEPKENGLVILVSVITSCLQTLKYCDSKLAALE
LILHLAPRLSVEILLDRITPYLLHFSNDSVPRVRAEALRTLTKVLALVKE
VPRNDINIYPEYILPGIAHLAQDDATIVRLAYAENIALLAETALRFLELV
QLKNLNMENDPNNEEIDEVTHPNGNYDTELQALHEMVQQKVVTLLSDPEN
IVKQTLMENGITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDS
IVGVAAYVGWQSSSILKPLLQQGLSDAEEFVIVKALYALTCMCQLGLLQK
PHVYEFASDIAPFLCHPNLWIRYGAVGFITVVARQISTADVYCKLMPYLD
PYITQPIIQIERKLVLLSVLKEPVSRSIFDYALRSKDITSLFRHLHMRQK
KRNGSLPDCPPPEDPAIAQLLKKLLSQGMTEEEEDKLLALKDFMMKSNKA
KANIVDQSHLHDSSQKGVIDLAALGITGRQVDLVKTKQEPDDKRARKHVK
QDSNVNEEWKSMFGSLDPPNMPQALPKGSDQEVIQTGKPPRSESSAGICV
PLSTSSQVPEVTTVQNKKPVIPVLSSTILPSTYQIRITTCKTELQQLIQQ
KREQCNAERIAKQMMENAEWESKPPPPGWRPKGLLVAHLHEHKSAVNRIR
VSDEHSLFATCSNDGTVKIWNSQKMEGKTTTTRSILTYSRIGGRVKTLTF
CQGSHYLAIASDNGAVQLLGIEASKLPKSPKIHPLQSRILDQKEDGCVVD
MHHFNSGAQSVLAYATVNGSLVGWDLRSSSNAWTLKHDLKSGLITSFAVD
IHQCWLCIGTSSGTMACWDMRFQLPISSHCHPSRARIRRLSMHPLYQSWV
IAAVQGNNEVSMWDMETGDRRFTLWASSAPPLSELQPSPHSVHGIYCSPA
DGNPILLTAGSDMKIRFWDLAYPERSYVVAGSTSSPSVSYYRKIIEGTEV
VQEIQNKQKVGPSDDTPRRGPESLPVGHHDIITDVATFQTTQGFIVTASR
DGIVKVWK*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8679
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Dual vector to express 6His VPS34mTOR and untagged VPS15 in baculovirus.