cDNA Clone TNF alpha

• Plasmid: pCMV-HA TNFalpha 77-234
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-TNFalpha 77-234
• Genbank: AAH28148.1
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGTCAGATCATCTTCTCGAACCCCGAGTGACAAGCCTGTAGCCCA
TGTTGTAGCAAACCCTCAAGCTGAGGGGCAGCTCCAGTGGCTGAACCGCC
GGGCCAATGCCCTCCTGGCCAATGGCGTGGAGCTGAGAGATAACCAGCTG
GTGGTGCCATCAGAGGGCCTGTACCTCATCTACTCCCAGGTCCTCTTCAA
GGGCCAAGGCTGCCCCTCCACCCATGTGCTCCTCACCCACACCATCAGCC
GCATCGCCGTCTCCTACCAGACCAAGGTCAACCTCCTCTCTGCCATCAAG
AGCCCCTGCCAGAGGGAGACCCCAGAGGGGGCTGAGGCCAAGCCCTGGTA
TGAGCCCATCTATCTGGGAGGGGTCTTCCAGCTGGAGAAGGGTGACCGAC
TCAGCGCTGAGATCAATCGGCCCGACTATCTCGACTTTGCCGAGTCTGGG
CAGGTCTACTTTGGGATCATTGCCCTGTGAGGATCC

Amino Acid Sequence

MYPYDVPDYAGSVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLA
NGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQ
TKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINR
PDYLDFAESGQVYFGIIAL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8656
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 18711 Daltons