cDNA Clone RIPK2

• Plasmid: pFBHTb RIPK2 2-311
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-RIPK2 2-311
• Genbank: NP_003812
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCCAACGGGGAGGCCATCTGCAGCGCCCTGCCCACCATTCCCTA
CCACAAACTCGCCGACCTGCGCTACCTGAGCCGCGGCGCCTCTGGCACTG
TGTCGTCCGCCCGCCACGCAGACTGGCGCGTCCAGGTGGCCGTGAAGCAC
CTGCACATCCACACTCCGCTGCTCGACAGTGAAAGAAAGGATGTCTTAAG
AGAAGCTGAAATTTTACACAAAGCTAGATTTAGTTACATTCTTCCAATTT
TGGGAATTTGCAATGAGCCTGAATTTTTGGGAATAGTTACTGAATACATG
CCAAATGGATCATTAAATGAACTCCTACATAGGAAAACTGAATATCCTGA
TGTTGCTTGGCCATTGAGATTTCGCATCCTGCATGAAATTGCCCTTGGTG
TAAATTACCTGCACAATATGACTCCTCCTTTACTTCATCATGACTTGAAG
ACTCAGAATATCTTATTGGACAATGAATTTCATGTTAAGATTGCAGATTT
TGGTTTATCAAAGTGGCGCATGATGTCCCTCTCACAGTCACGAAGTAGCA
AATCTGCACCAGAAGGAGGGACAATTATCTATATGCCACCTGAAAACTAT
GAACCTGGACAAAAATCAAGGGCCAGTATCAAGCACGATATATATAGCTA
TGCAGTTATCACATGGGAAGTGTTATCCAGAAAACAGCCTTTTGAAGATG
TCACCAATCCTTTGCAGATAATGTATAGTGTGTCACAAGGACATCGACCT
GTTATTAATGAAGAAAGTTTGCCATATGATATACCTCACCGAGCACGTAT
GATCTCTCTAATAGAAAGTGGATGGGCACAAAATCCAGATGAAAGACCAT
CTTTCTTAAAATGTTTAATAGAACTTGAACCAGTTTTGAGAACATTTGAA
GAGATAACTTTTCTTGAAGCTGTTATTCAGCTAAAGAAATAAAAGTTACA
GAGTGTTTCAAGTGCCATTCACCTATGTGACAAGAAGAAAATGGAATTAT
CTCTGAACATACCTGTAAATCATGGTCCACAAGAGGAATCATGTGGATCC
TCTCAGCTCCATGAAAATAGTGGTTCTCCTGAAACTTCAAGGTCCCTGCC
AGCTCCTCAAGACAATGATTTTTTATCTAGAAAAGCTCAAGACTGTTATT
TTATGAAGCTGCATCACTGTCCTGGAAATCACAGTTGGGATAGCACCATT
TCTGGATCTCAAAGGGCTGCATTCTGTGATCACAAGACCACTCCATGCTC
TTCAGCAATAATAAATCCACTCTCAACTGCAGGAAACTCAGAACGTCTGC
AGCCTGGTATAGCCCAGCAGTGGATCCAGAGCAAAAGGGAAGACATTGTG
AACCAAATGACAGAAGCCTGCCTTAACCAGTCGCTAGATGCCCTTCTGTC
CAGGGACTTGATCATGAAAGAGGACTATGAACTTGTTAGTACCAAGCCTA
CAAGGACCTCAAAAGTCAGACAATTACTAGACACTACTGACATCCAAGGA
GAAGAATTTGCCAAAGTTATAGTACAAAAATTGAAAGATAACAAACAAAT
GGGTCTTCAGCCTTACCCGGAAATACTTGTGGTTTCTAGATCACCATCTT
TAAATTTACTTCAAAATAAAAGCATGTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSGIQRPTSTSSLVAAANGEAICSALPTIPYHKLADLRYLSRGASGTVSSARHADWRVQVAVKHLHIHTPLLDSERKDVLREAEILHKARFSYILPILGICNEPEFLGIVTEYMPNGSLNELLHRKTEYPDVAWPLRFRILHEIALGVNYLHNMTPPLLHHDLKTQNILLDNEFHVKIADFGLSKWRMMSLSQSRSSKSAPEGGTIIYMPPENYEPGQKSRASIKHDIYSYAVITWEVLSRKQPFEDVTNPLQIMYSVSQGHRPVINEESLPYDIPHRARMISLIESGWAQNPDERPSFLKCLIELEPVLRTFEEITFLEAVIQLKK*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8644
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; N.B. sequence change compared with sequence NP_003812: t1200a (silent in G400). This change present in EST sequences.