cDNA Clone TNF alpha

• Plasmid: YFP-N1 TNFalpha
• Parent Plasmid: pEYFPN1
• Expressed: TNFalpha YFP
• Genbank: NP_000585.2
• Tag: YFP
• Species: Human

Sequence of Insert

GTCGACATGAGCACTGAAAGCATGATCCGGGACGTGGAGCTGGCCGAGGA
GGCGCTCCCCAAGAAGACAGGGGGGCCCCAGGGCTCCAGGCGGTGCTTGT
TCCTCAGCCTCTTCTCCTTCCTGATCGTGGCAGGCGCCACCACGCTCTTC
TGCCTGCTGCACTTTGGAGTGATCGGCCCCCAGAGGGAAGAGTTCCCCAG
GGACCTCTCTCTAATCAGCCCTCTGGCCCAGGCAGTCAGATCATCTTCTC
GAACCCCGAGTGACAAGCCTGTAGCCCATGTTGTAGCAAACCCTCAAGCT
GAGGGGCAGCTCCAGTGGCTGAACCGCCGGGCCAATGCCCTCCTGGCCAA
TGGCGTGGAGCTGAGAGATAACCAGCTGGTGGTGCCATCAGAGGGCCTGT
ACCTCATCTACTCCCAGGTCCTCTTCAAGGGCCAAGGCTGCCCCTCCACC
CATGTGCTCCTCACCCACACCATCAGCCGCATCGCCGTCTCCTACCAGAC
CAAGGTCAACCTCCTCTCTGCCATCAAGAGCCCCTGCCAGAGGGAGACCC
CAGAGGGGGCTGAGGCCAAGCCCTGGTATGAGCCCATCTATCTGGGAGGG
GTCTTCCAGCTGGAGAAGGGTGACCGACTCAGCGCTGAGATCAATCGGCC
CGACTATCTCGACTTTGCCGAGTCTGGGCAGGTCTACTTTGGGATCATTG
CCCTGTCACGGGATCC

Amino Acid Sequence

MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCL
LHFGVIGPQREEFPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEG
QLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHV
LLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVF
QLEKGDRLSAEINRPDYLDFAESGQVYFGIIALSRDPPVATMVSKGEELF
TGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWP
TLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKT
RAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQK
NGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALS
KDPNEKRDHMVLLEFVTAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU8635
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C Ter YFP for mammalian expression.; ; Estimated MW of expressed protein : 53440 Daltons