cDNA Clone RIPK2

• Plasmid: pCMV-HA RIPK2 S174D S180D S181D
• Parent Plasmid: pEBG6P
• Expressed: HA-RIPK2 S174D S180D S181D
• Genbank: NP_003812
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCCAACGGGGAGGCCATCTGCAGCGCCCTGCCCACCATTCCCTA
CCACAAACTCGCCGACCTGCGCTACCTGAGCCGCGGCGCCTCTGGCACTG
TGTCGTCCGCCCGCCACGCAGACTGGCGCGTCCAGGTGGCCGTGAAGCAC
CTGCACATCCACACTCCGCTGCTCGACAGTGAAAGAAAGGATGTCTTAAG
AGAAGCTGAAATTTTACACAAAGCTAGATTTAGTTACATTCTTCCAATTT
TGGGAATTTGCAATGAGCCTGAATTTTTGGGAATAGTTACTGAATACATG
CCAAATGGATCATTAAATGAACTCCTACATAGGAAAACTGAATATCCTGA
TGTTGCTTGGCCATTGAGATTTCGCATCCTGCATGAAATTGCCCTTGGTG
TAAATTACCTGCACAATATGACTCCTCCTTTACTTCATCATGACTTGAAG
ACTCAGAATATCTTATTGGACAATGAATTTCATGTTAAGATTGCAGATTT
TGGTTTATCAAAGTGGCGCATGATGGACCTCTCACAGTCACGAGATGACA
AATCTGCACCAGAAGGAGGGACAATTATCTATATGCCACCTGAAAACTAT
GAACCTGGACAAAAATCAAGGGCCAGTATCAAGCACGATATATATAGCTA
TGCAGTTATCACATGGGAAGTGTTATCCAGAAAACAGCCTTTTGAAGATG
TCACCAATCCTTTGCAGATAATGTATAGTGTGTCACAAGGACATCGACCT
GTTATTAATGAAGAAAGTTTGCCATATGATATACCTCACCGAGCACGTAT
GATCTCTCTAATAGAAAGTGGATGGGCACAAAATCCAGATGAAAGACCAT
CTTTCTTAAAATGTTTAATAGAACTTGAACCAGTTTTGAGAACATTTGAA
GAGATAACTTTTCTTGAAGCTGTTATTCAGCTAAAGAAAACAAAGTTACA
GAGTGTTTCAAGTGCCATTCACCTATGTGACAAGAAGAAAATGGAATTAT
CTCTGAACATACCTGTAAATCATGGTCCACAAGAGGAATCATGTGGATCC
TCTCAGCTCCATGAAAATAGTGGTTCTCCTGAAACTTCAAGGTCCCTGCC
AGCTCCTCAAGACAATGATTTTTTATCTAGAAAAGCTCAAGACTGTTATT
TTATGAAGCTGCATCACTGTCCTGGAAATCACAGTTGGGATAGCACCATT
TCTGGATCTCAAAGGGCTGCATTCTGTGATCACAAGACCACTCCATGCTC
TTCAGCAATAATAAATCCACTCTCAACTGCAGGAAACTCAGAACGTCTGC
AGCCTGGTATAGCCCAGCAGTGGATCCAGAGCAAAAGGGAAGACATTGTG
AACCAAATGACAGAAGCCTGCCTTAACCAGTCGCTAGATGCCCTTCTGTC
CAGGGACTTGATCATGAAAGAGGACTATGAACTTGTTAGTACCAAGCCTA
CAAGGACCTCAAAAGTCAGACAATTACTAGACACTACTGACATCCAAGGA
GAAGAATTTGCCAAAGTTATAGTACAAAAATTGAAAGATAACAAACAAAT
GGGTCTTCAGCCTTACCCGGAAATACTTGTGGTTTCTAGATCACCATCTT
TAAATTTACTTCAAAATAAAAGCATGTAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAANGEAICSALPTIPYHKLADLRYLSRGA
SGTVSSARHADWRVQVAVKHLHIHTPLLDSERKDVLREAEILHKARFSYI
LPILGICNEPEFLGIVTEYMPNGSLNELLHRKTEYPDVAWPLRFRILHEI
ALGVNYLHNMTPPLLHHDLKTQNILLDNEFHVKIADFGLSKWRMMDLSQS
RDDKSAPEGGTIIYMPPENYEPGQKSRASIKHDIYSYAVITWEVLSRKQP
FEDVTNPLQIMYSVSQGHRPVINEESLPYDIPHRARMISLIESGWAQNPD
ERPSFLKCLIELEPVLRTFEEITFLEAVIQLKKTKLQSVSSAIHLCDKKK
MELSLNIPVNHGPQEESCGSSQLHENSGSPETSRSLPAPQDNDFLSRKAQ
DCYFMKLHHCPGNHSWDSTISGSQRAAFCDHKTTPCSSAIINPLSTAGNS
ERLQPGIAQQWIQSKREDIVNQMTEACLNQSLDALLSRDLIMKEDYELVS
TKPTRTSKVRQLLDTTDIQGEEFAKVIVQKLKDNKQMGLQPYPEILVVSR
SPSLNLLQNKSM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8437
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express RICK with N terminal HA.; N.B. sequence change compared with sequence NP_003812: t1200a (silent in G400). This change present in EST sequences.; ; Estimated MW of expressed protein : 63510 Daltons