cDNA Clone MST4

• Plasmid: pFBHTb MST4
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-MST4
• Genbank: NP_057626
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCCCACTCGCCGGTGGCTGTCCAAGTGCCTGGGATGCAGAA
TAACATAGCTGATCCAGAAGAACTGTTCACAAAATTAGAGCGCATTGGGA
AAGGCTCATTTGGGGAAGTTTTCAAAGGAATTGATAACCGTACCCAGCAA
GTCGTTGCTATTAAAATCATAGACCTTGAGGAAGCCGAAGATGAAATAGA
AGACATTCAGCAAGAAATAACTGTCTTGAGTCAATGTGACAGCTCATATG
TAACAAAATACTATGGGTCATATTTAAAGGGGTCTAAATTATGGATAATA
ATGGAATACCTGGGCGGTGGTTCAGCACTGGATCTTCTTCGAGCTGGTCC
ATTTGATGAGTTCCAGATTGCTACCATGCTAAAGGAAATTTTAAAAGGTC
TGGACTATCTGCATTCAGAAAAGAAAATTCACCGAGACATAAAAGCTGCC
AATGTCTTGCTCTCAGAACAAGGAGATGTTAAACTTGCTGATTTTGGAGT
TGCTGGTCAGCTGACAGATACACAGATTAAAAGAAATACCTTTGTGGGAA
CTCCATTTTGGATGGCTCCTGAAGTTATTCAACAGTCAGCTTATGACTCA
AAAGCTGACATTTGGTCATTGGGAATTACTGCTATTGAACTAGCCAAGGG
AGAGCCACCTAACTCCGATATGCATCCAATGAGAGTTCTGTTTCTTATTC
CCAAAAACAATCCTCCAACTCTTGTTGGAGACTTTACTAAGTCTTTTAAG
GAGTTTATTGATGCTTGCCTGAACAAAGATCCATCATTTCGTCCTACAGC
AAAAGAACTTCTGAAACACAAATTCATTGTAAAAAATTCAAAGAAGACTT
CTTATCTGACTGAACTGATAGATCGTTTTAAGAGATGGAAGGCAGAAGGA
CACAGTGATGATGAATCTGATTCCGAGGGCTCTGATTCGGAATCTACCAG
CAGGGAAAACAATACTCATCCTGAATGGAGCTTTACCACCGTACGAAAGA
AGCCTGATCCAAAGAAAGTACAGAATGGGGCAGAGCAAGATCTTGTGCAA
ACCCTGAGTTGTTTGTCTATGATAATCACACCTGCATTTGCTGAACTTAA
ACAGCAGGACGAGAATAACGCTAGCAGGAATCAGGCGATTGAAGAACTCG
AGAAAAGTATTGCTGTGGCTGAAGCCGCCTGTCCCGGCATCACAGATAAA
ATGGTGAAGAAACTAATTGAAAAATTTCAAAAGTGTTCAGCAGACGAATC
CCCCTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMAHSPVAVQVPGMQNNIADPEE
LFTKLERIGKGSFGEVFKGIDNRTQQVVAIKIIDLEEAEDEIEDIQQEIT
VLSQCDSSYVTKYYGSYLKGSKLWIIMEYLGGGSALDLLRAGPFDEFQIA
TMLKEILKGLDYLHSEKKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDT
QIKRNTFVGTPFWMAPEVIQQSAYDSKADIWSLGITAIELAKGEPPNSDM
HPMRVLFLIPKNNPPTLVGDFTKSFKEFIDACLNKDPSFRPTAKELLKHK
FIVKNSKKTSYLTELIDRFKRWKAEGHSDDESDSEGSDSESTSRENNTHP
EWSFTTVRKKPDPKKVQNGAEQDLVQTLSCLSMIITPAFAELKQQDENNA
SRNQAIEELEKSIAVAEAACPGITDKMVKKLIEKFQKCSADESP*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8430
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His for baculovirus expression.; ; Estimated MW of expressed protein : 49898 Daltons; ; All MST4 plasmids MUST be grown at 30 degrees C or less to prevent recombination.