cDNA Clone 14-3-3 epsilon

• Synonyms: YWHAE
• Plasmid: pET28a 14-3-3 epsilon
• Parent Plasmid: pET28a original
• Expressed: 6His-14-3-3 epsilon
• Genbank: NP_006752.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGATGATCGAGAGGATCTGGTGTACCAGGCGAAGCTGGCCGA
GCAGGCTGAGCGATACGACGAAATGGTGGAGTCAATGAAGAAAGTAGCAG
GGATGGATGTGGAGCTGACAGTTGAAGAAAGAAACCTCCTATCTGTTGCA
TATAAGAATGTGATTGGAGCTAGAAGAGCCTCCTGGAGAATAATCAGCAG
CATTGAACAGAAAGAAGAAAACAAGGGAGGAGAAGACAAGCTAAAAATGA
TTCGGGAATATCGGCAAATGGTTGAGACTGAGCTAAAGTTAATCTGTTGT
GACATTCTGGATGTACTGGACAAACACCTCATTCCAGCAGCTAACACTGG
CGAGTCCAAGGTTTTCTATTATAAAATGAAAGGGGACTACCACAGGTATC
TGGCAGAATTTGCCACAGGAAACGACAGGAAGGAGGCTGCGGAGAACAGC
CTAGTGGCTTATAAAGCTGCTAGTGATATTGCAATGACAGAACTTCCACC
AACGCATCCTATTCGCTTAGGTCTTGCTCTCAATTTTTCCGTATTCTACT
ACGAAATTCTTAATTCCCCTGACCGTGCCTGCAGGTTGGCAAAAGCAGCT
TTTGATGATGCAATTGCAGAACTGGATACGCTGAGTGAAGAAAGCTATAA
GGACTCTACACTTATCATGCAGTTGTTACGTGATAATCTGACACTATGGA
CTTCAGACATGCAGGGTGACGGTGAAGAGCAGAATAAAGAAGCGCTGCAG
GACGTGGAAGACGAAAATCAGTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMDDREDLVYQAKLAEQ
AERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSI
EQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGE
SKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPT
HPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKD
STLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU8326
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for bacterial expression.; ; Estimated MW of expressed protein : 32716 Daltons