cDNA Clone 14-3-3 zeta

• Synonyms: YWHAZ
• Plasmid: pET28a 14-3-3 zeta
• Parent Plasmid: pET28a original
• Expressed: 6His-14-3-3 zeta
• Genbank: BC013265
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGATAAAAATGAGCTGGTTCAGAAGGCCAAACTGGCCGAGCA
GGCTGAGCGATATGATGACATGGCAGCCTGCATGAAGTCTGTAACTGAGC
AAGGAGCTGAATTATCCAATGAGGAGAGGAATCTTCTCTCAGTTGCTTAT
AAAAATGTTGTAGGAGCCCGTAGGTCATCTTGGAGGGTCGTCTCAAGTAT
TGAACAAAAGACGGAAGGTGCTGAGAAAAAACAGCAGATGGCTCGAGAAT
ACAGAGAGAAAATTGAGACGGAGCTAAGAGATATCTGCAATGATGTACTG
TCTCTTTTGGAAAAGTTCTTGATCCCCAATGCTTCACAAGCAGAGAGCAA
AGTCTTCTATTTGAAAATGAAAGGAGATTACTACCGTTACTTGGCTGAGG
TTGCCGCTGGTGATGACAAGAAAGGGATTGTCGATCAGTCACAACAAGCA
TACCAAGAAGCTTTTGAAATCAGCAAAAAGGAAATGCAACCAACACATCC
TATCAGACTGGGTCTGGCCCTTAACTTCTCTGTGTTCTATTATGAGATTC
TGAACTCCCCAGAGAAAGCCTGCTCTCTTGCAAAGACAGCTTTTGATGAA
GCCATTGCTGAACTTGATACATTAAGTGAAGAGTCATACAAAGACAGCAC
GCTAATAATGCAATTACTGAGAGACAACTTGACATTGTGGACATCGGATA
CCCAAGGAGACGAAGCTGAAGCAGGAGAAGGAGGGGAAAATTAAGCGGCC
GC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMDKNELVQKAKLAEQA
ERYDDMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARRSSWRVVSSIE
QKTEGAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQAESKV
FYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPI
RLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTL
IMQLLRDNLTLWTSDTQGDEAEAGEGGEN*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU8266
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for bacterial expression.; Estimated MW of expressed protein : 31287 Daltons