cDNA Clone 14-3-3 gamma

• Synonyms: YWHAG
• Plasmid: pET28a 14-3-3 gamma
• Parent Plasmid: pET28a original
• Expressed: 6His-14-3-3 gamma
• Genbank: AAH20963.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGTGGACCGCGAGCAACTGGTGCAGAAAGCCCGGCTGGCCGA
GCAGGCGGAGCGCTACGACGACATGGCCGCGGCCATGAAGAACGTGACAG
AGCTGAATGAGCCACTGTCGAATGAGGAACGAAACCTTCTGTCTGTGGCC
TACAAGAACGTTGTGGGGGCACGCCGCTCTTCCTGGAGGGTCATCAGTAG
CATTGAGCAGAAGACATCTGCAGACGGCAATGAGAAGAAGATTGAGATGG
TCCGTGCGTACCGGGAGAAGATAGAGAAGGAGTTGGAGGCTGTGTGCCAG
GATGTGCTGAGCCTGCTGGATAACTACCTGATCAAGAATTGCAGCGAGAC
CCAGTACGAGAGCAAAGTGTTCTACCTGAAGATGAAAGGGGACTACTACC
GCTACCTGGCTGAAGTGGCCACCGGAGAGAAAAGGGCGACGGTGGTGGAG
TCCTCTGAGAAGGCCTACAGCGAAGCCCACGAGATCAGCAAAGAGCACAT
GCAGCCCACCCACCCCATCCGATTAGGCCTGGCTCTTAACTACTCCGTCT
TCTACTATGAGATCCAGAACGCCCCAGAGCAAGCGTGCCACTTGGCCAAG
ACCGCGTTCGACGACGCCATCGCCGAGCTTGACACCCTCAACGAGGACTC
CTACAAGGACTCCACGCTCATCATGCAGCTCCTCCGCGACAACCTCACGC
TCTGGACGAGCGACCAGCAGGACGACGATGGCGGCGAAGGCAACAATTAA
GCGGCCGC

Amino Acid Sequence

MGSSHHHHHSSGLVPRGSHMASMTGGQQMGRGSMVDREQLVQKARLAEQA
ERYDDMAAAMKNVTELNEPLSNEERNLLSVAYKNVVGARRSSWRVISSIE
QKTSADGNEKKIEMVRAYREKIEKELEAVCQDVLSLLDNYLIKNCSETQY
ESKVFYLKMKGDYYRYLAEVATGEKRATVVESSEKAYSEAHEISKEHMQP
THPIRLGLALNYSVFYYEIQNAPEQACHLAKTAFDDAIAELDTLNEDSYK
DSTLIMQLLRDNLTLWTSDQQDDDGGEGNN*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU8262
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for bacterial expression.; ; Estimated MW of expressed protein : 31707 Da