cDNA Clone SLX4 (yeast)

• Plasmid: pACT2 SLX4
• Parent Plasmid: pACT2
• Expressed: GAL4-AD 6His-SLX4
• Genbank: Z73307
• Tag: GAL4 DNA-AD
• Species: Saccharomyces cerevisiae

Sequence of Insert

ccatggGCAGCAGCCATCACCATCATCACCACAGCCAGGATCCGGAACTTCAGAGGGCACAACGGAATTTGAAGTTTTTACAAAACGAGGATTATGTGAACGTTACCGATCAAACTAACCTGAATGGAGAGAGCCAGAATGCTTACTCTTTAGGAATGGAAACTCAAGTACCGGAAATGCAGTTTTCCTTATCATCTGATGATGATTCAATAGGTACGCAAGTAAAAAGCGTAACTGCACAAAAATCGCCTATGACGCAAGAGACTACGAAAAACGATACAGAAAGAAATAAAGATGTTGATAAAAGTTGTAACCCAGTCAGCACTTCCCATCCCGATTTAGGCGGATCAAATATCGAGGAGAACATATTCATTAACACTCAAATTCAAAGCCGACTTGATGACGCAGAGGAAGAAACAAATCTTAAGTTAAAACTTGAAAAATTCAAGTATTCGTTCAAGAGTAGTAACGCAGATGATACCCACAGCAATGCAAATGTAACAGCTAAAAGAAGACCAGCAATAAGGAAGGCTAATTCGAAACTCAAAACAAAACCTAAAACTAAACGAGACCCTAAAATTATCAAAAATATAACTGATTTCAATATTAATAACTACGAACGTTCAAGGACTGCCAGCTTATTAAAGCAATTATCAGGTAAGCACAAGAAGGTTTTGGACATAATAAAGACGCAAAATGAAGGAAACAGTGATAAACCTCCCAGAGCAAGGAATAACAAGGGTGAAAAAGCTACTTTTGATACATATAGTGAACAGGAGTGGAAAGATATTATGAAACTTCTTCTACAAAAGTTTCCGCAAAGTGAGGAAACGGATTTGAATGAAGTCCAGAAATTTTTATATGGAAGCGAAAAAAGCTCGAATTCTCTAGACAATCAAGAATCCTCACAACAAAGACTTTGGACAGCATCACAATTACCACCCGAGCTTCCCGATGAGGCTATTCAACCAGAGCAGGAAGAAAGGATACGCGATACCCAAAGTGCAGTAAATTTCTTATCGTTGTCTCAAGTAATGGATGACAAAAGTGAAATTATGAAAGACGAAGAAAGCATAATTATTTCACGGGGAGATTCTACAAGCTCTCAAGAATATGGAAACGGGTTAGAGCCACAACAACCGGTAGGAAATGTTGTTGGTGAAGATATTGAACTGGCTGTGGGAACACGTATAAATGCATTTTCACTGACAGATTATAAAGCTTGCAAACCTATGTCTGTTGAGGTAAGTAGAAGATGCGAAAACAGTACGGATAATGATTATGACAATATTTCTATTGTCTCAGATACTACAGATGAGACATCCACATTGTTTCCACTAGACCAGTATCGGTATGTTTTCATTGAAAATGATGAAAGACCACCATTAGCCACCGACACGATAGGCAGCACACAGTTTTTCACTCCAAACACATCGCCTTTGGATGGGATAATCGATTTAACACAGGAATCATTCAAAGCTGTTCGATCGTTAATATCACCTTTGAAAGTAGAAAACAACAAAACAGGGGTAACATCACAAGCATCAAATCAGGTTCAGGTACCAGCTACAAGAACCCCTACAATTATTCCTCAAAAGAACCTTACTACAACTTTAAAAACTGAAGAAGAAAAGAATAACATAGGAAGTTCCATCAGAGTTAAATTATTGCAAGAAAGCGTAGTTAAGCTAAACCCCAAACTGGTGAAACATAACTTTTATAGAGTTGAGGCAAATGATTCTGAAGAAGAGGAGACAGAATTTGATGACCAATTCTGCATTGCTGATATCCAATTGGTAGATTCCTCCAAAATTTCGACTAAAGATTCTACACAAAATCCTACTACTAGCAACGACATTATTGATACCTCTGCAGCTAGTTCCATAGCTTCTCCCGAGAAATTTTGTGAGATCATGATGTCCCAATCAATGAAAGAGCTTCGACAAAGCTTGAAAACAGTGGGGTTGAAACCTATGAGGACGAAGGTTGAAATCATTCAATCCTTACAAACTGCATCTCAAATTCTGTCCACAGCTAATCCAGATAATAAAGGTGAGCATGGTGGGGTGGCAAATTTCAGTAAGATAGAAATATTTGATCATTTAACCGAACTAATTGAAGCCTTCCCTGATTTTTTGGAGCGAATATATACCTTTGAACCCATTCCGCTAAATGAGCTAATTGAAAAGCTGTTCAGCGCCGAGCCATTTGTTTCGCAAATCGACGAAATGACCATCAGAGAATGGGCCGATGTTCAAGGAATATGTCTGAGAAACGATAAAAAGTAGGGATCCGAATTCGAGCTCGGCGCGCCTGCAGGTCGACAAGCTT

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEICMAYPYDVPDYASLGGHMAMGSSHHHHHHSQDPELQRAQRNLKFLQNEDYVNVTDQTNLNGESQNAYSLGMETQVPEMQFSLSSDDDSIGTQVKSVTAQKSPMTQETTKNDTERNKDVDKSCNPVSTSHPDLGGSNIEENIFINTQIQSRLDDAEEETNLKLKLEKFKYSFKSSNADDTHSNANVTAKRRPAIRKANSKLKTKPKTKRDPKIIKNITDFNINNYERSRTASLLKQLSGKHKKVLDIIKTQNEGNSDKPPRARNNKGEKATFDTYSEQEWKDIMKLLLQKFPQSEETDLNEVQKFLYGSEKSSNSLDNQESSQQRLWTASQLPPELPDEAIQPEQEERIRDTQSAVNFLSLSQVMDDKSEIMKDEESIIISRGDSTSSQEYGNGLEPQQPVGNVVGEDIELAVGTRINAFSLTDYKACKPMSVEVSRRCENSTDNDYDNISIVSDTTDETSTLFPLDQYRYVFIENDERPPLATDTIGSTQFFTPNTSPLDGIIDLTQESFKAVRSLISPLKVENNKTGVTSQASNQVQVPATRTPTIIPQKNLTTTLKTEEEKNNIGSSIRVKLLQESVVKLNPKLVKHNFYRVEANDSEEEETEFDDQFCIADIQLVDSSKISTKDSTQNPTTSNDIIDTSAASSIASPEKFCEIMMSQSMKELRQSLKTVGLKPMRTKVEIIQSLQTASQILSTANPDNKGEHGGVANFSKIEIFDHLTELIEAFPDFLERIYTFEPIPLNELIEKLFSAEPFVSQIDEMTIREWADVQGICLRNDKK*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU8253
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 2 hybrid construct.; ; All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.