cDNA Clone UBCH10

• Plasmid: pET28a ubcH10
• Parent Plasmid: pET28a original
• Expressed: 6His-ubcH10
• Genbank: NP_008950
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCTTCCCAAAACCGCGACCCAGCCGCCACTAGCGTCGCCGC
CGCCCGTAAAGGAGCTGAGCCGAGCGGGGGCGCCGCCCGGGGTCCGGTGG
GCAAAAGGCTACAGCAGGAGCTGATGACCCTCATGATGTCTGGCGATAAA
GGGATTTCTGCCTTCCCTGAATCAGACAACCTTTTCAAATGGGTAGGGAC
CATCCATGGAGCAGCTGGAACAGTATATGAAGACCTGAGGTATAAGCTCT
CGCTAGAGTTCCCCAGTGGCTACCCTTACAATGCGCCCACAGTGAAGTTC
CTCACGCCCTGCTATCACCCCAACGTGGACACCCAGGGTAACATATGCCT
GGACATCCTGAAGGAAAAGTGGTCTGCCCTGTATGATGTCAGGACCATTC
TGCTCTCCATCCAGAGCCTTCTAGGAGAACCCAACATTGATAGTCCCTTG
AACACACATGCTGCCGAGCTCTGGAAAAACCCCACAGCTTTTAAGAAGTA
CCTGCAAGAAACCTACTCAAAGCAGGTCACCAGCCAGGAGCCCTGAGCGG
CCGC

Amino Acid Sequence

MGSSLHHHHHSSGLVPRGSHMASMTGGQQMGRGSMASQNRDPAATSVAAA
RKGAEPSGGAARGPVGKRLQQELMTLMMSGDKGISAFPESDNLFKWVGTI
HGAAGTVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLD
ILKEKWSALYDVRTILLSIQSLLGEPNIDSPLNTHAAELWKNPTAFKKYL
QETYSKQVTSQEP*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU8231
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His tag for bacterial expression.; Estimated MW of expressed protein : 23171 Daltons