cDNA Clone VPS15

• Plasmid: pFB VPS15 6His
• Parent Plasmid: pFastbac1
• Expressed: 6His-VPS15 6His
• Genbank: NP_055417
• Tag: 6His
• Species: Human

Sequence of Insert

GTCGACGAAACCATGGGAAATCAGCTTGCTGGCATTGCTCCCTCCCAGAT
CCTTTCTGTAGAGAGTTATTTTTCAGATATTCATGACTTTGAATATGATA
AAAGCCTGGGGAGTACTCGGTTTTTTAAAGTTGCTCGAGCCAAGCACCGA
GAAGGCCTGGTCGTTGTGAAGGTTTTTGCAATTCAGGATCCCACATTGCC
TTTAACCAGCTATAAACAAGAGCTGGAGGAACTGAAAATCAGGCTTAATT
CTGCACAGAATTGTCTACCTTTCCAGAAAGCATCAGAAAAAGCATCTGAG
AAAGCAGCTATGCTCTTTAGGCAGTATGTGCGAGACAATCTCTATGATCG
CATCAGTACCCGTCCATTCTTGAATAACATTGAGAAGCGCTGGATTGCTT
TCCAGATCCTGACAGCTGTGGACCAAGCACACAAATCTGGAGTTCGTCAT
GGGGACATCAAGACTGAGAATGTGATGGTCACCAGTTGGAATTGGGTTCT
TCTAACTGATTTTGCCAGTTTTAAGCCCACTTATCTTCCAGAAGACAACC
CGGCAGATTTCAATTATTTCTTTGACACATCACGGAGGAGAACTTGCTAT
ATTGCTCCTGAACGTTTTGTTGATGGTGGGATGTTTGCCACTGAGTTAGA
ATATATGAGAGATCCTTCAACTCCGCTTGTAGACTTAAATAGCAATCAGA
GAACAAGAGGAGAGTTGAAGAGAGCAATGGACATCTTTTCAGCAGGTTGT
GTGATAGCTGAGCTTTTTACAGAAGGTGTACCATTATTTGATCTCTCTCA
ACTTTTGGCTTATAGAAATGGACATTTTTTCCCTGAACAAGTGCTAAATA
AAATTGAAGATCACAGTATCAGAGAATTGGTAACTCAGATGATTCACCGT
GAGCCAGATAAACGTTTAGAGGCAGAAGATTACTTAAAACAGCAGCGTGG
CAATGCCTTTCCTGAAATATTTTACACTTTTCTTCAGCCCTACATGGCCC
AGTTTGCCAAGGAAACGTTTCTTTCTGCAGATGAGCGTATTCTGGTTATA
CGGAAGGATTTGGGCAACATTATTCACAATCTCTGTGGACATGATCTGCC
AGAAAAAGCCGAAGGAGAGCCTAAGGAAAATGGGCTGGTTATCTTGGTAT
CTGTTATAACATCCTGCCTACAGACCCTTAAATACTGTGATTCCAAACTA
GCTGCTTTGGAACTGATTCTTCATTTGGCTCCAAGATTAAGTGTTGAAAT
CCTTTTGGATCGTATTACTCCATATCTTTTGCATTTCAGCAATGACTCTG
TTCCTAGGGTGAGGGCTGAAGCCTTGAGGACGTTGACCAAAGTTCTTGCT
CTCGTCAAAGAGGTTCCTCGTAATGATATCAATATTTATCCGGAATACAT
TCTGCCAGGCATAGCCCACTTAGCCCAAGATGATGCTACTATCGTTAGAC
TAGCCTATGCTGAAAACATAGCTCTGCTGGCAGAAACAGCTCTGAGATTC
CTGGAATTAGTACAGTTAAAAAATCTTAATATGGAAAATGACCCCAATAA
TGAAGAAATAGATGAGGTTACACATCCAAATGGAAATTATGACACAGAGC
TCCAAGCCTTACATGAAATGGTCCAGCAGAAAGTTGTTACTTTGCTAAGT
GACCCTGAAAATATTGTAAAACAAACCTTGATGGAAAATGGAATAACACG
GCTGTGTGTATTCTTTGGACGTCAGAAAGCCAACGATGTTTTGTTGTCCC
ACATGATTACTTTCCTAAATGATAAGAATGATTGGCATCTACGTGGAGCA
TTTTTTGATAGTATAGTTGGTGTTGCTGCCTATGTTGGCTGGCAAAGCTC
CTCAATTCTCAAGCCTCTGCTGCAACAAGGTCTTAGTGATGCTGAGGAAT
TTGTCATTGTGAAAGCTCTTTATGCCCTTACTTGTATGTGCCAGTTAGGA
CTGCTACAAAAACCCCATGTTTACGAATTTGCCAGTGATATTGCCCCCTT
CCTGTGTCATCCCAATTTATGGATACGTTATGGTGCCGTGGGATTTATCA
CAGTGGTAGCTCGTCAAATAAGTACAGCTGATGTCTACTGTAAACTGATG
CCTTATCTTGACCCATATATTACCCAACCAATAATACAGATTGAAAGAAA
ACTTGTTCTGCTCAGTGTTTTAAAGGAACCAGTAAGTCGTTCTATATTTG
ATTATGCTTTGAGGTCTAAAGATATTACTAGCTTGTTCAGACATCTTCAC
ATGCGTCAGAAGAAACGAAATGGTTCTCTTCCCGACTGCCCTCCGCCAGA
GGATCCTGCCATAGCACAGCTTCTGAAGAAGTTGCTCTCACAGGGAATGA
CAGAGGAAGAGGAAGACAAACTTCTGGCACTGAAAGACTTCATGATGAAA
TCTAATAAAGCAAAGGCCAATATAGTGGACCAGAGCCATCTTCATGATAG
TAGTCAGAAAGGTGTAATTGACTTGGCAGCTTTAGGCATAACTGGGAGAC
AAGTTGATCTTGTTAAAACCAAACAAGAACCAGATGACAAACGGGCCAGA
AAACATGTAAAACAAGACTCAAATGTAAATGAAGAATGGAAAAGCATGTT
TGGGTCACTGGACCCACCAAACATGCCACAGGCCCTACCTAAAGGGAGTG
ATCAGGAGGTGATTCAGACTGGGAAACCTCCTCGTTCCGAGTCCTCTGCT
GGCATTTGTGTCCCTTTGTCAACTTCTTCACAGGTTCCAGAAGTGACAAC
TGTCCAAAATAAAAAACCAGTAATACCGGTTTTAAGTAGTACAATCTTAC
CATCCACCTATCAGATTCGAATTACAACTTGTAAAACTGAACTTCAGCAA
CTCATCCAGCAAAAGCGGGAGCAGTGCAATGCTGAGAGAATAGCTAAGCA
GATGATGGAAAATGCTGAATGGGAGAGTAAACCACCACCACCTGGATGGC
GTCCTAAAGGGCTGTTAGTTGCCCATCTTCATGAGCATAAATCTGCTGTG
AATCGAATTAGAGTCTCTGATGAACACTCACTTTTTGCAACATGTTCAAA
TGATGGCACAGTGAAAATCTGGAACAGTCAAAAGATGGAGGGGAAGACCA
CCACTACCAGATCTATTCTTACATACAGCCGAATTGGAGGACGAGTCAAG
ACGCTCACATTCTGCCAAGGCTCCCACTATTTAGCCATAGCATCTGATAA
TGGTGCTGTCCAGCTTCTTGGAATTGAGGCTTCTAAGCTGCCCAAGTCTC
CTAAAATCCATCCTCTACAAAGCAGAATTCTAGATCAGAAGGAGGACGGT
TGTGTTGTGGATATGCATCACTTCAACTCTGGAGCACAGTCTGTTCTTGC
CTATGCCACTGTGAATGGCTCTCTGGTTGGCTGGGACCTTAGGTCTTCAA
GCAATGCGTGGACTTTAAAGCATGATTTAAAGTCGGGCCTCATCACTTCC
TTTGCTGTGGACATCCACCAATGCTGGCTCTGCATTGGTACAAGCAGTGG
TACCATGGCTTGTTGGGACATGAGGTTCCAGTTGCCAATTTCAAGTCACT
GTCATCCTTCCAGGGCTCGAATCAGACGCCTCTCAATGCACCCTCTGTAT
CAGTCCTGGGTGATTGCAGCTGTTCAGGGCAACAACGAAGTGTCCATGTG
GGACATGGAGACTGGTGACAGAAGATTTACTCTCTGGGCCAGCAGTGCAC
CACCACTTTCTGAATTACAGCCTTCTCCTCATAGCGTCCATGGTATCTAC
TGTAGTCCTGCAGATGGAAATCCTATCCTACTAACAGCTGGCTCAGATAT
GAAAATAAGGTTTTGGGACTTGGCTTACCCAGAAAGGTCCTATGTTGTTG
CAGGAAGTACTAGTTCCCCATCTGTGTCCTACTACAGGAAAATAATTGAA
GGCACTGAAGTTGTCCAGGAAATTCAGAATAAGCAGAAAGTAGGACCAAG
TGATGACACCCCTCGAAGGGGCCCAGAGTCCCTGCCCGTGGGACATCATG
ACATCATCACTGATGTCGCCACATTCCAGACCACACAGGGCTTCATCGTA
ACTGCTTCTAGAGATGGGATTGTGAAGGTGTGGAAACATCATCACCATCA
CCATTAAGCGGCCGC

Amino Acid Sequence

MGNQLAGIAPSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAKHREGLV
VVKVFAIQDPTLPLTSYKQELEELKIRLNSAQNCLPFQKASEKASEKAAM
LFRQYVRDNLYDRISTRPFLNNIEKRWIAFQILTAVDQAHKSGVRHGDIK
TENVMVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPE
RFVDGGMFATELEYMRDPSTPLVDLNSNQRTRGELKRAMDIFSAGCVIAE
LFTEGVPLFDLSQLLAYRNGHFFPEQVLNKIEDHSIRELVTQMIHREPDK
RLEAEDYLKQQRGNAFPEIFYTFLQPYMAQFAKETFLSADERILVIRKDL
GNIIHNLCGHDLPEKAEGEPKENGLVILVSVITSCLQTLKYCDSKLAALE
LILHLAPRLSVEILLDRITPYLLHFSNDSVPRVRAEALRTLTKVLALVKE
VPRNDINIYPEYILPGIAHLAQDDATIVRLAYAENIALLAETALRFLELV
QLKNLNMENDPNNEEIDEVTHPNGNYDTELQALHEMVQQKVVTLLSDPEN
IVKQTLMENGITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDS
IVGVAAYVGWQSSSILKPLLQQGLSDAEEFVIVKALYALTCMCQLGLLQK
PHVYEFASDIAPFLCHPNLWIRYGAVGFITVVARQISTADVYCKLMPYLD
PYITQPIIQIERKLVLLSVLKEPVSRSIFDYALRSKDITSLFRHLHMRQK
KRNGSLPDCPPPEDPAIAQLLKKLLSQGMTEEEEDKLLALKDFMMKSNKA
KANIVDQSHLHDSSQKGVIDLAALGITGRQVDLVKTKQEPDDKRARKHVK
QDSNVNEEWKSMFGSLDPPNMPQALPKGSDQEVIQTGKPPRSESSAGICV
PLSTSSQVPEVTTVQNKKPVIPVLSSTILPSTYQIRITTCKTELQQLIQQ
KREQCNAERIAKQMMENAEWESKPPPPGWRPKGLLVAHLHEHKSAVNRIR
VSDEHSLFATCSNDGTVKIWNSQKMEGKTTTTRSILTYSRIGGRVKTLTF
CQGSHYLAIASDNGAVQLLGIEASKLPKSPKIHPLQSRILDQKEDGCVVD
MHHFNSGAQSVLAYATVNGSLVGWDLRSSSNAWTLKHDLKSGLITSFAVD
IHQCWLCIGTSSGTMACWDMRFQLPISSHCHPSRARIRRLSMHPLYQSWV
IAAVQGNNEVSMWDMETGDRRFTLWASSAPPLSELQPSPHSVHGIYCSPA
DGNPILLTAGSDMKIRFWDLAYPERSYVVAGSTSSPSVSYYRKIIEGTEV
VQEIQNKQKVGPSDDTPRRGPESLPVGHHDIITDVATFQTTQGFIVTASR
DGIVKVWKHHHHHH*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8126
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C terminal 6His tag for baculovirus expression.; Estimated MW of expressed protein : 153921 Daltons