cDNA Clone EMAPL

• Plasmid: pCMV-HA EMAPL1-268 T199A
• Parent Plasmid: pCMV5 3HA
• Expressed: HA-EMAPL 1-268 T199A
• Genbank: AK093146
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCATGGAACTGGTAAAGGCAGCCCTGGCAGAAGCCCTTCGCCTGCT
GCGGCTGCAGGTGCCCCCTTCCTCCCTGCAGGGCTCTGGCACACCAGCTC
CTCCGGGGGACAGTCTTGCAGCCCCCCCAGGACTGCCACCCACGTGCACC
CCTTCCTTGGTGAGCCGAGGCACCCAGACGGAGACAGAGGTGGAGCTCAA
GTCATCCCCTGGACCCCCTGGCCTGAGCAATGGACCCCCAGCCCCTCAGG
GGGCCAGCGAAGAGCCTAGCGGGACCCAATCTGAAGGAGGGGGCAGCAGC
AGCAGTGGTGCTGGCTCCCCTGGCCCCCCGGGGATCCTCAGGCCCTTGCA
GCCCCCACAGCGTGCTGACACGCCGCGAAGAAATTCTTCCTCCTCCTCAT
CCCCCTCAGAGCGGCCTCGGCAGAAGCTCTCCAGGAAGGCAATCTCCTCC
GCCAACCTGTTAGTGCGGTCCGGGAGCACAGAGAGCCGTGGGGGAAAAGA
CCCCCTCTCCAGCCCTGGGGGCCCTGGATCTCGGAGGAGCAATTACAATT
TGGAAGGCATCTCAGTGAAGATGTTCCTTCGAGGGCGCCCCATTGCCATG
TACATCCCGTCTGGCATCCGCAGCCTTGAGGAGCTGCCGAGTGGCCCACC
GCCAGAGACCCTCAGCCTTGACTGGGTTTATGGGTACAGGGGTCGTGACT
CCCGCTCCAATCTGTTTGTGTTGCGCTCTGGGGAGGTGGTCTACTTTATC
GCCTGTGTGGTGGTGCTGTACCGGCCTGGAGGAGGCCCAGGGGGTCCTGG
AGGTGGCGGCTAGGAATTC

Amino Acid Sequence

MYPYDVPDYAMYPYDVPDYAMYPYDVPDYAEFMELVKAALAEALRLLRLQ
VPPSSLQGSGTPAPPGDSLAAPPGLPPTCTPSLVSRGTQTETEVELKSSP
GPPGLSNGPPAPQGASEEPSGTQSEGGGSSSSGAGSPGPPGILRPLQPPQ
RADTPRRNSSSSSSPSERPRQKLSRKAISSANLLVRSGSTESRGGKDPLS
SPGGPGSRRSNYNLEGISVKMFLRGRPIAMYIPSGIRSLEELPSGPPPET
LSLDWVYGYRGRDSRSNLFVLRSGEVVYFIACVVVLYRPGGGPGGPGGGG
*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8087
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence differences compared with AK093146 CDS:; delta cag 108-110 (delta S36)*, ; t705c (silent in S235 (S234 in cloned fragment)); * present in other published mRNA sequences for EMAPL, e.g. XP_508493, BC075824, BC050467, AL833388; ; Estimated MW of expressed protein : 31340 Daltons