cDNA Clone PIAS3

• Plasmid: pCMV-HA-1 PIAS3
• Parent Plasmid: pCMV5 HA2
• Expressed: HA-PIAS3
• Genbank: AAH51252
• Tag: HA
• Species: Mouse

Sequence of Insert

GCGGCCGCATGGCGGAGCTGGGCGAGTTAAAGCACATGGTGATGAGTTTC
CGAGTGTCTGAGCTCCAGGTGCTCCTCGGCTTCGCTGGCAGGAACAAGAG
TGGGCGGAAACACGAGCTGCTGGCCAAGGCCCTGCACCTCCTCAAGTCTA
GCTGCGCCCCCAGCGTCCAGATGAAGATCAAAGAACTTTATCGCAGGCGC
TTTCCCCGGAAGACCCTGGGGCCTTCTGATCTCTCCTTGCTTTCTTTGCC
CCCTGGCACCTCTCCTGTAGGCTCCCCCGGCCCCCTTGCTCCCATCCCTC
CCACCCTTCTGACCCCTGGCACCTTGCTAGGCCCTAAGCGTGAGGTGGAC
ATGCATCCTCCTCTGCCGCAGCCTGTGCACCCCGATGTCACCATGAAGCC
ACTGCCCTTCTATGAAGTCTATGGGGAGCTCATCCGACCCACCACCCTTG
CGTCCACCTCCAGCCAGAGGTTCGAGGAAGCCCACTTCACCTTCGCGCTC
ACTCCCCAGCAGCTGCAGCAGATTCTCACGTCCAGGGAGGTTCTGCCAGG
AGCCAAGTGTGATTACACCATACAAGTGCAGCTCAGATTCTGTCTCTGTG
AGACCAGCTGCCCTCAGGAGGACTATTTCCCCCCTAACCTCTTTGTTAAG
GTTAATGGGAAACTCTGCCCCCTGCCGGGTTACCTCCCTCCAACCAAGAA
TGGAGCTGAGCCCAAGAGGCCCAGCCGTCCGATCAACATCACACCCTTGG
CTCGACTCTCAGCCACTGTCCCCAACACCATCGTAGTTAATTGGTCATCT
GAGTTTGGACGGAATTACTCCTTGTCCGTGTACCTGGTGAGGCAATTGAC
TGCAGGGACCCTTCTACAAAAACTCAGAGCCAAGGGGATCCGGAATCCAG
ACCATTCCCGGGCACTGATCAAGGAGAAACTGACTGCTGACCCCGACAGT
GAAGTGGCTACTACAAGTCTCCGGGTGTCACTCATGTGCCCGCTAGGGAA
GATGCGCCTGACTGTCCCGTGTCGTGCCCTCACCTGTGCCCATCTGCAGA
GTTTCGATGCTGCCCTTTATCTACAGATGAATGAGAAGAAGCCGACATGG
ACGTGTCCTGTGTGTGACAAGAAGGCTCCCTATGAGTCGCTGATTATTGA
TGGTTTATTCATGGAAATTCTTAATTCCTGTTCGGATTGTGATGAGATCC
AGTTCATGGAAGATGGATCCTGGTGTCCGATGAAACCCAAGAAGGAGGCA
TCAGAGGTTTGCCCCCCGCCAGGGTATGGGCTGGATGGTCTCCAGTACAG
CGCAGTCCAGGAGGGAATTCAGCCAGAGAGTAAGAAGAGGGTCGAAGTCA
TTGACTTGACCATCGAAAGCTCATCAGATGAGGAGGATTTGCCCCCCACC
AAGAAGCACTGCCCTGTCACCTCAGCGGCCATTCCAGCCCTTCCTGGAAG
CAAAGGAGCCCTGACCTCTGGTCACCAGCCATCCTCGGTGCTGCGGAGCC
CTGCAATGGGCACACTGGGCAGTGACTTCCTGTCTAGTCTCCCGCTACAT
GAGTACCCACCTGCCTTCCCACTGGGGGCTGACATCCAAGGTTTAGATTT
ATTTTCTTTCCTTCAGACTGAGAGTCAGCACTACGGCCCTTCAGTTATCA
CTTCGCTAGATGAACAGGACACCCTTGGCCACTTCTTCCAGTACCGGGGA
ACCCCTTCCCACTTCCTGGGCCCACTGGCCCCCACACTGGGGAGCTCTCA
CCGCAGCTCCACTCCAGCGCCCCCTCCTGGTCGTGTCAGCAGCATTGTGG
CTCCTGGGAGCTCCTTGAGGGAAGGGCATGGAGGACCCCTGCCTTCAGGT
CCCTCTTTGACTGGCTGTCGGTCAGACGTCATTTCCTTGGACTGAGCGGC
CGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMAELGELKHMVMSFRVSELQVLLGFAG
RNKSGRKHELLAKALHLLKSSCAPSVQMKIKELYRRRFPRKTLGPSDLSL
LSLPPGTSPVGSPGPLAPIPPTLLTPGTLLGPKREVDMHPPLPQPVHPDV
TMKPLPFYEVYGELIRPTTLASTSSQRFEEAHFTFALTPQQLQQILTSRE
VLPGAKCDYTIQVQLRFCLCETSCPQEDYFPPNLFVKVNGKLCPLPGYLP
PTKNGAEPKRPSRPINITPLARLSATVPNTIVVNWSSEFGRNYSLSVYLV
RQLTAGTLLQKLRAKGIRNPDHSRALIKEKLTADPDSEVATTSLRVSLMC
PLGKMRLTVPCRALTCAHLQSFDAALYLQMNEKKPTWTCPVCDKKAPYES
LIIDGLFMEILNSCSDCDEIQFMEDGSWCPMKPKKEASEVCPPPGYGLDG
LQYSAVQEGIQPESKKRVEVIDLTIESSSDEEDLPPTKKHCPVTSAAIPA
LPGSKGALTSGHQPSSVLRSPAMGTLGSDFLSSLPLHEYPPAFPLGADIQ
GLDLFSFLQTESQHYGPSVITSLDEQDTLGHFFQYRGTPSHFLGPLAPTL
GSSHRSSTPAPPPGRVSSIVAPGSSLREGHGGPLPSGPSLTGCRSDVISL
D*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8071
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N Terminal HA for mammalian expression.; Estiamted MW of expressed protein : 70637 Daltons