cDNA Clone VASP

• Plasmid: pEGFP-C1-VASP S322A
• Parent Plasmid: pEGFPC1
• Expressed: GFP-VASP S322A
• Genbank: CAA67147
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTCATACGA
TGTGCCAGATTACGCCAGCGAGACGGTCATCTGTTCCAGCCGGGCCACTG
TGATGCTTTATGATGATGGCAACAAGCGATGGCTCCCTGCTGGCACGGGT
CCCCAGGCCTTCAGCCGCGTCCAGATCTACCACAACCCCACGGCCAATTC
CTTTCGCGTCGTGGGCCGGAAGATGCAGCCCGACCAGCAGGTGGTCATCA
ACTGTGCCATCGTCCGGGGTGTCAAGTATAACCAGGCCACCCCCAACTTC
CATCAGTGGCGCGACGCTCGCCAGGTCTGGGGCCTCAACTTCGGCAGCAA
GGAGGATGCGGCCCAGTTTGCCGCCGGCATGGCCAGTGCCCTAGAGGCGT
TGGAAGGAGGTGGGCCCCCTCCACCCCCAGCACTTCCCACCTGGTCGGTC
CCGAACGGCCCCTCCCCGGAGGAGGTGGAGCAGCAGAAAAGGCAGCAGCC
CGGCCCGTCGGAGCACATAGAGCGCCGGGTCTCCAATGCAGGAGGCCCAC
CTGCTCCCCCCGCTGGGGGTCCACCCCCACCACCAGGACCTCCCCCTCCT
CCAGGTCCCCCCCCACCCCCAGGTTTGCCCCCTTCGGGGGTCCCAGCTGC
AGCGCACGGAGCAGGGGGAGGACCACCCCCTGCACCCCCTCTCCCGGCAG
CACAGGGCCCTGGTGGTGGGGGAGCTGGGGCCCCAGGCCTGGCCGCAGCT
ATTGCTGGAGCCAAACTCAGGAAAGTCAGCAAGCAGGAGGAGGCCTCAGG
GGGGCCCACAGCCCCCAAAGCTGAGAGTGGTCGAAGCGGAGGTGGGGGAC
TCATGGAAGAGATGAACGCCATGCTGGCCCGGAGAAGGAAAGCCACGCAA
GTTGGGGAGAAAACCCCCAAGGATGAATCTGCCAATCAGGAGGAGCCAGA
GGCCAGAGTCCCGGCCCAGAGTGAATCTGTGCGGAGACCCTGGGAGAAGA
ACAGCACAACCTTGCCAAGGATGAAGGCGTCTTCTTCGGTGACCACTTCC
GAGACCCAACCCTGCACGCCCAGCTCCAGTGATTACTCGGACCTACAGAG
GGTGAAACAGGAGCTTTTGGAAGAGGTGAAGAAGGAATTGCAGAAAGTGA
AAGAGGAAATCATTGAAGCCTTCGTCCAGGAGCTGAGGAAGCGGGGTTCT
CCCTGAGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSTSNGRQCAGIRPSYDVPDYASETVICSSRATVMLYDDGN
KRWLPAGTGPQAFSRVQIYHNPTANSFRVVGRKMQPDQQVVINCAIVRGV
KYNQATPNFHQWRDARQVWGLNFGSKEDAAQFAAGMASALEALEGGGPPP
PPALPTWSVPNGPSPEEVEQQKRQQPGPSEHIERRVSNAGGPPAPPAGGP
PPPPGPPPPPGPPPPPGLPPSGVPAAAHGAGGGPPPAPPLPAAQGPGGGG
AGAPGLAAAIAGAKLRKVSKQEEASGGPTAPKAESGRSGGGGLMEEMNAM
LARRRKATQVGEKTPKDESANQEEPEARVPAQSESVRRPWEKNSTTLPRM
KSSSSVTTSETQPCTPSSSDYSDLQRVKQELLEEVKKELQKVKEEIIEAF
VQELRKRGSP*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU8027
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP tag for mammalian expression.