cDNA Clone USP2 (rat)

• Plasmid: pGEX6P-1-USP2 rat 271-618
• Parent Plasmid: pGEX6P1
• Expressed: GST USP2 rat 271-618
• Genbank: BC085719.1
• Tag: GST
• Species: Rat

Sequence of Insert

GGATCCATGAATTCAAAGAGTGCCCAGGGTCTGGCTGGTCTTCGAAACCT
TGGGAACACGTGCTTCATGAACTCAATTCTTCAGTGTCTGAGCAACACCC
GCGAGTTGAGAGATTACTGCCTCCAGAGGCTGTACATGCGGGACCTCGGC
CATACCAGCAGTGCACACACAGCTCTCATGGAAGAGTTTGCAAAACTAAT
CCAGACCATATGGACGTCATCCCCCAATGATGTGGTGAGCCCATCTGAGT
TCAAGACCCAGATCCAGAGATATGCACCACGCTTCGTTGGCTATAATCAG
CAGGATGCTCAGGAATTCCTCCGTTTCCTTCTGGATGGCCTCCACAACGA
GGTGAACCGGGTGGCAGCAAGGCCTAAGCCCAGCCCTGAGAGCCTTGATC
ATCTCCCTGATGAAGAGAAAGGGCGACAGATGTGGAGGAAGTATCTAGAA
AGGGAAGACAGTCGGATTGGGGATCTCTTCGTTGGGCAGCTAAAGAGCTC
CCTGACGTGCACCGACTGCGGCTACTGCTCTACAGTGTTCGATCCCTTCT
GGGACCTCTCGTTGCCCATTGCAAAGAGAGGCTATCCTGAAGTGACGTTA
ATGGATTGTATGAGGCTCTTCACCAAAGAGGATGTGTTGGATGGGGATGA
GAAACCAACATGCTGCCGCTGCCGGGCCAGAAAACGATGTATAAAGAAGT
TCTCTGTCCAGAGGTTCCCAAAGATCTTGGTGCTCCACCTGAAGCGGTTC
TCAGAATCCAGGATACGAACCAGCAAGCTCACAACATTTGTGAATTTCCC
ACTAAGAGACCTGGACTTGAGAGAATTTGCTTCAGAAAACACCAACCATG
CTGTTTACAACCTGTATGCTGTGTCCAATCACTCCGGAACCACCATGGGA
GGTCACTATACAGCCTACTGCCGAAGTCCGGTTACGGGCGAATGGCACAC
TTTCAATGACTCCAGTGTCACACCCATGTCCTCCAGCCAAGTGCGCACCA
GCGACGCCTATTTGCTCTTCTATGAACTGGCCAGTCCACCCTCCCGTATG
TAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNSKSAQGLAGLRNLGNTC
FMNSILQCLSNTRELRDYCLQRLYMRDLGHTSSAHTALMEEFAKLIQTIW
TSSPNDVVSPSEFKTQIQRYAPRFVGYNQQDAQEFLRFLLDGLHNEVNRV
AARPKPSPESLDHLPDEEKGRQMWRKYLEREDSRIGDLFVGQLKSSLTCT
DCGYCSTVFDPFWDLSLPIAKRGYPEVTLMDCMRLFTKEDVLDGDEKPTC
CRCRARKRCIKKFSVQRFPKILVLHLKRFSESRIRTSKLTTFVNFPLRDL
DLREFASENTNHAVYNLYAVSNHSGTTMGGHYTAYCRSPVTGEWHTFNDS
SVTPMSSSQVRTSDAYLLFYELASPPSRM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU35692
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 66815 Daltons