cDNA Clone Cyclin Y

• Plasmid: pGEX6P-1-Cyclin Y
• Parent Plasmid: pGEX6P1
• Expressed: GST Cyclin Y
• Genbank: NP_659449.3
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGGGAACACTACCTCGTGCTGCGTGTCGTCCAGTCCCAAGCT
CCGGAGGAATGCCCACTCCCGGCTGGAGTCCTACCGGCCAGACACGGACC
TGAGCCGCGAGGACACGGGCTGCAACCTGCAGCACATCAGCGACCGGGAG
AACATAGACGATTTGAACATGGAATTCAATCCTTCAGATCATCCTCGGGC
CAGCACAATATTCCTCAGTAAATCTCAGACGGACGTGAGAGAAAAACGCA
AGAGTCTCTTCATTAACCATCATCCTCCAGGACAAATAGCAAGGAAATAC
AGTTCCTGCTCCACCATTTTCCTAGATGATAGCACAGTCAGTCAACCAAA
CCTCAAGTATACAATTAAATGTGTCGCTCTTGCAATATATTATCACATCA
AAAACAGGGACCCAGATGGAAGGATGCTCTTAGATATTTTTGATGAAAAT
CTTCACCCTCTTTCGAAATCCGAAGTGCCACCAGATTATGACAAACACAA
CCCAGAGCAGAAGCAGATTTACCGGTTCGTTCGGACACTGTTCAGTGCTG
CTCAGCTGACGGCTGAATGTGCCATCGTCACCCTGGTGTACCTTGAAAGA
CTTTTAACATACGCAGAGATAGATATCTGTCCGGCCAACTGGAAGCGGAT
TGTTTTAGGGGCGATCCTGCTGGCCTCCAAGGTGTGGGATGACCAGGCTG
TATGGAATGTGGATTACTGCCAGATCCTGAAAGACATCACGGTGGAGGAC
ATGAACGAGCTAGAGCGACAGTTTCTTGAATTGCTGCAGTTCAACATCAA
TGTTCCTTCCAGTGTCTATGCCAAGTATTATTTTGATCTTCGTTCTCTGG
CAGAAGCGAACAACCTGAGCTTTCCCTTGGAGCCCCTGAGCAGGGAGAGG
GCTCACAAGCTTGAGGCCATCTCTCGCCTCTGCGAGGACAAGTACAAGGA
CCTAAGAAGATCCGCGAGGAAGCGCTCAGCCAGTGCAGACAACCTGACTC
TGCCCCGGTGGTCCCCAGCCATCATCTCTTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGNTTSCCVSSSPKLRRNA
HSRLESYRPDTDLSREDTGCNLQHISDRENIDDLNMEFNPSDHPRASTIF
LSKSQTDVREKRKSLFINHHPPGQIARKYSSCSTIFLDDSTVSQPNLKYT
IKCVALAIYYHIKNRDPDGRMLLDIFDENLHPLSKSEVPPDYDKHNPEQK
QIYRFVRTLFSAAQLTAECAIVTLVYLERLLTYAEIDICPANWKRIVLGA
ILLASKVWDDQAVWNVDYCQILKDITVEDMNELERQFLELLQFNINVPSS
VYAKYYFDLRSLAEANNLSFPLEPLSRERAHKLEAISRLCEDKYKDLRRS
ARKRSASADNLTLPRWSPAIIS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU35643
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 66118 Daltons