cDNA Clone PTPN12

• Plasmid: pEBG6P-PTPN12
• Parent Plasmid: pEBG6P
• Expressed: GST PTPN12
• Genbank: AAH50008.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAGCAAGTGGAGATCCTGAGGAAATTCATCCAGAGGGTCCA
GGCCATGAAGAGTCCTGACCACAATGGGGAGGACAACTTCGCCCGGGACT
TCATGCGGTTAAGAAGATTGTCTACCAAATATAGAACAGAAAAGATATAT
CCCACAGCCACTGGAGAAAAAGAAGAAAATGTTAAAAAGAACAGATACAA
GGACATACTGCCATTTGATCACAGCCGAGTTAAATTGACATTAAAGACTC
CTTCACAAGATTCAGACTATATCAATGCAAATTTTATAAAGGGCGTCTAT
GGGCCAAAAGCATATGTAGCAACTCAAGGACCTTTAGCAAATACAGTAAT
AGATTTTTGGAGGATGATATGGGAGTATAATGTTGTGATCATTGTAATGG
CCTGCCGAGAATTTGAGATGGGAAGGAAAAAATGTGAGCGCTATTGGCCT
TTGTATGGAGAAGACCCCATAACGTTTGCACCATTTAAAATTTCTTGTGA
GGATGAACAAGCAAGAACAGACTACTTCATCAGGACACTCTTACTTGAAT
TTCAAAATGAATCTCGTAGGCTGTATCAGTTTCATTATGTGAACTGGCCA
GACCATGATGTTCCTTCATCATTTGATTCTATTCTGGACATGATAAGCTT
AATGAGGAAATATCAAGAACATGAAGATGTTCCTATTTGTATTCATTGCA
GTGCAGGCTGTGGAAGAACAGGTGCCATTTGTGCCATAGATTATACGTGG
AATTTACTAAAAGCTGGGAAAATACCAGAGGAATTTAATGTATTTAATTT
AATACAAGAAATGAGAACACAAAGGCATTCTGCAGTACAAACAAAGGAGC
AATATGAACTTGTTCATAGAGCTATTGCCCAACTGTTTGAAAAACAGCTA
CAACTATATGAAATTCATGGAGCTCAGAAAATTGCTGATGGAGTGAATGA
AATTAACACTGAAAACATGATCAGCTCCATAGAGCCTGAAAAACAAGATT
CTCCTCCTCCAAAACCACCAAGGACCCGCAGTTGCCTTGTTGAAGGGGAT
GCTAAAGAAGAAATACTGCAGCCACCGGAACCTCATCCAGTGCCACCCAT
CTTGACACCTTCTCCCCCTTCAGCTTTTCCAACAGTCACTACTGTGTGGC
AGGACAATGATAGATACCATCCAAAGCCAGTGTTGCATATGGTTTCATCA
GAACAACATTCAGCAGACCTCAACAGAAACTATAGTAAATCAACAGAACT
TCCAGGGAAAAATGAATCAACAATTGAACAGATAGATAAAAAATTGGAAC
GAAATTTAAGTTTTGAGATTAAGAAGGTCCCTCTCCAAGAGGGACCAAAA
AGTTTTGATGGGAACACACTTTTGAATAGGGGACATGCAATTAAAATTAA
ATCTGCTTCACCTTGTATAGCTGATAAAATCTCTAAGCCACAGGAATTAA
GTTCAGATCTAAATGTCGGTGATACTTCCCAGAATTCTTGTGTGGACTGC
AGTGTAACACAATCAAACAAAGTTTCAGTTACTCCACCAGAAGAATCCCA
GAATTCAGACACACCTCCAAGGCCAGACCGCTTGCCTCTTGATGAGAAAG
GACATGTAACGTGGTCATTTCATGGACCTGAAAATGCCATACCCATACCT
GATTTATCTGAAGGCAATTCCTCAGATATCAACTATCAAACTAGGAAAAC
TGTGAGTTTAACACCAAGTCCTACAACACAAGTTGAAACACCTGATCTTG
TGGATCATGATAACACTTCACCACTCTTCAGAACACCCCTCAGTTTTACT
AATCCACTTCACTCTGATGACTCAGACTCAGATGAAAGAAACTCTGATGG
TGCTGTGACCCAGAATAAAACTAATATTTCAACAGCAAGTGCCACAGTTT
CTGCTGCCACTAGTACTGAAAGCATTTCTACTAGGAAAGTATTGCCAATG
TCCATTGCTAGACATAATATAGCAGGAACAACACATTCAGGTGCTGAAAA
AGATGTTGATGTTAGTGAAGATTCACCTCCTCCCCTACCTGAAAGAACTC
CTGAATCGTTTGTGTTAGCAAGTGAACATAATACACCTGTAAGATCGGAA
TGGAGTGAACTTCAAAGTCAGGAACGATCTGAACAAAAAAAGTCTGAAGG
CTTGATAACCTCTGAAAATGAGAAATGTGATCATCCAGCGGGAGGTATTC
ACTATGAAATGTGCATAGAATGTCCACCTACTTTCAGTGACAAGAGAGAA
CAAATATCAGAAAATCCAACAGAAGCCACAGATATTGGTTTTGGTAATCG
ATGTGGAAAACCCAAAGGACCAAGAGATCCACCTTCAGAATGGACATGAG
CGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMEQVEILRKFIQR
VQAMKSPDHNGEDNFARDFMRLRRLSTKYRTEKIYPTATGEKEENVKKNR
YKDILPFDHSRVKLTLKTPSQDSDYINANFIKGVYGPKAYVATQGPLANT
VIDFWRMIWEYNVVIIVMACREFEMGRKKCERYWPLYGEDPITFAPFKIS
CEDEQARTDYFIRTLLLEFQNESRRLYQFHYVNWPDHDVPSSFDSILDMI
SLMRKYQEHEDVPICIHCSAGCGRTGAICAIDYTWNLLKAGKIPEEFNVF
NLIQEMRTQRHSAVQTKEQYELVHRAIAQLFEKQLQLYEIHGAQKIADGV
NEINTENMISSIEPEKQDSPPPKPPRTRSCLVEGDAKEEILQPPEPHPVP
PILTPSPPSAFPTVTTVWQDNDRYHPKPVLHMVSSEQHSADLNRNYSKST
ELPGKNESTIEQIDKKLERNLSFEIKKVPLQEGPKSFDGNTLLNRGHAIK
IKSASPCIADKISKPQELSSDLNVGDTSQNSCVDCSVTQSNKVSVTPPEE
SQNSDTPPRPDRLPLDEKGHVTWSFHGPENAIPIPDLSEGNSSDINYQTR
KTVSLTPSPTTQVETPDLVDHDNTSPLFRTPLSFTNPLHSDDSDSDERNS
DGAVTQNKTNISTASATVSAATSTESISTRKVLPMSIARHNIAGTTHSGA
EKDVDVSEDSPPPLPERTPESFVLASEHNTPVRSEWSELQSQERSEQKKS
EGLITSENEKCDHPAGGIHYEMCIECPPTFSDKREQISENPTEATDIGFG
NRCGKPKGPRDPPSEWT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU35599
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression; ; Estimated MW of expressed protein : 115535 Daltons