cDNA Clone MYPT1 (rat); PP1 beta

• Plasmid: POPH-His-PP1 beta, GST-rat wt MYPT1
• Parent Plasmid: pOPH 6His
• Expressed: His-PP1 beta, GST-rat wt MYPT1
• Genbank: PP1 beta BT019744(human AA), NM_001101722 (rabbit) MYPT1: NP_446342
• Tag: GST

Sequence of Insert

DNA sequence of rabbit PP1 beta

ATGGCTCATCATCACCATCACCACATGGAAAACCTGTATTTTCAGGGCCATATGGCGGACGGGGAGCTGAACGTGGACAGCCTCATCACCCGCCTGCTGGAGGTACGAGGATGTCGTCCAGGAAAGATTGTGCAAATGACTGAAGCGGAAGTGCGGGGCCTTTGTATCAAGTCTCGGGAGATCTTCCTCAGCCAGCCTATTCTCTTGGAATTGGAAGCACCACTGAAAATTTGTGGAGATATTCATGGACAGTATACAGATTTACTGAGATTATTTGAGTATGGAGGTTTCCCACCAGAAGCCAACTATCTTTTCTTAGGAGATTATGTGGATAGAGGAAAACAGTCTTTGGAAACCATTTGTTTGCTATTGGCTTACAAAATCAAATATCCAGAGAACTTCTTTCTCTTAAGAGGAAACCATGAGTGTGCTAGCATCAATCGCATTTATGGATTCTATGATGAATGCAAGCGAAGGTTTAATATTAAATTGTGGAAGACCTTCACTGACTGCTTTAACTGTCTGCCTATAGCTGCCATTGTGGATGAGAAGATCTTCTGTTGTCATGGAGGACTGTCACCAGACCTACAATCTATGGAGCAGATTCGGAGAATTATGAGACCCACTGATGTCCCTGATACAGGTTTGCTCTGTGATTTACTGTGGTCTGACCCAGACAAGGACGTGCAAGGCTGGGGAGAAAATGACCGTGGTGTTTCCTTCACTTTTGGCGCTGATGTAGTCAGTAAATTTCTGAATCGTCATGATTTAGACCTGATTTGTCGAGCTCATCAGGTGGTGGAAGATGGATATGAATTTTTTGCTAAGCGACAATTGGTAACCCTATTTTCAGCCCCAAATTACTGTGGCGAGTTTGATAATGCTGGTGGAATGATGAGTGTGGATGAGACTTTGATGTGTTCATTTCAGATATTGAAACCATCTGAAAAGAAAGCTAAATACCAGTATGGTGGACTGAATTCTGGACGCCCTGTCACTCCACCTCGAACAGCTAATCCGCCGAAGAAAAGGTGAGGATCCAGATCTAAGCTTGGTACC

GST-MYPT1
GGATCCAATAATTTTGTTTAACTTTAAGAAGGAGATATAAATATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTCGGAGCCCCAGGAATTCCCGGGGCGACTCGAGCGGCCGCGATGAAGATGGCGGACGCGAAGCAGAAGCGGAACGAGCAGCTGAAGCGCTGGATCGGCTCCGAGACGGACCTCGAGCCTCCCGTGGTGAAGCGCCAGAAGACCAAGGTGAAGTTCGACGATGGCGCCGTCTTCCTCGCCGCCTGCTCCAGCGGCGACACGGACGAGGTCCTCAAGCTGCTGCACCGCGGCGCCGACATCAATTACGCCAATGTGGACGGACTCACCGCCCTGCACCAGGCTTGCATTGATGACAATGTTGATATGGTGAAGTTTCTGGTAGAAAATGGAGCAAATATCAATCAACCTGACAATGAAGGCTGGATTCCACTCCATGCAGCCGCTTCCTGTGGATATCTGGATATTGCAGAATTTTTGATTGGTCAAGGAGCACATGTAGGAGCTGTCAACAGTGAAGGTGACACACCTTTAGATATTGCAGAGGAGGAAGCAATGGAAGAGCTACTTCAAAATGAGGTTAATCGGCAAGGTGTTGATATAGAAGCAGCTCGAAAAGAAGAGGAACGCATAATGCTTAGAGACGCGAGGCAGTGGTTGAACAGTGGTCACATCAGTGACGTCCGGCATGCAAAGTCCGGAGGCACAGCACTCCACGTGGCAGCGGCCAAAGGGTATACAGAAGTTTTAAAACTTTTAATACAGGCAGGCTATGATGTTAATATTAAAGATTATGATGGCTGGACACCTCTTCATGCTGCAGCTCACTGGGGTAAAGAAGAAGCATGTCGGATTTTAGTGGACAATCTGTGTGATATGGAGACGGTCAACAAAGTGGGCCAAACAGCCTTTGATGTAGCAGATGAAGACATTTTGGGATATCTAGAGGAGTTGCAAAAAAAACAAAATCTGCTCCATAGTGAAAAGCGGGATAAGAAATCTCCACTGATTGAATCAACAGCAAATATGGAAAATAATCAACCACAGAAGACTTTTAAAAACAAGGAAACGTTGATTATTGAGCCAGAGAAAAATGCATCTCGAATCGAGTCTCTGGAGCAAGAAAAGGCTGATGAGGAGGAGGAAGGCAAGAAGGATGAGTCCAGCTGCTCCAGTGAGGAGGATGAGGAGGATGACTCCGAGTCCGAAGCGGAGACAGATAAGACAAAACCCATGGCTTCTGTAACTAATGCTCACACTGCCAGCACTCAGGCAGCTCCTGCCGCTGTGACAACACCTACTCTGTCTTCCAACCAGGGGACCCCTACATCACCTGTTAAAAAGTTTCCTACATCAACTACAAAAATTTCTCCCAAAGAAGAAGAAAGAAAAGATGAATCTCCTGCATCCTGGAGGTTAGGACTTAGAAAGACTGGCAGTTATGGTGCCCTGGCTGAGATCACTGCATCTAAAGAAGCTCAGAAGGAGAAAGACACTGCAGGCGTGATACGTTCAGCTTCGAGTCCCAGACTCTCGTCCTCTTTGGATAATAAAGAAAAGGAGAAAGACAATAAAGGAACAAGACTTGCATATGTCGCCCCTACAATCCCAAGGCGACTAGGCAGTACGTCTGACATTGAAGAGAAGGAAAACAGAGAGTCTTCAAATTTGCGAACAAGTAGTTCTTACACAAGAAGAAAATGGGAAGATGATCTTAAAAAAAATAGTTCAATCAATGAAGGATCTACTTACCATAGAAGTACCTCAAATCGTTTGTGGGCTGAGGATAGTACTGAGAAAGAGAAGGACAGTGCTCCTACCGCAGCGACCATTCTTGTTGCTCCAACTGTTGTAAGTGCTGCAGCTTCTTCTACCACAGCCCTGACCACAACTACTGCTGGCACTCTTTCCTCCACATCAGAGGTCAGGGAGAGACGCAGGTCATACCTCACTCCTGTTAGGGATGAAGAGTCTGAATCCCAAAGGAAAGCAAGATCTAGACAAGCAAGACAGTCTAGACGGTCAACACAGGGGGTGACACTGACTGACCTCCAGGAAGCCGAAAAGACAATAGGAAGAAGCCGTTCTACGAGAACCAGAGAACAAGAAAACGAAGAAAAAGACAAAGAAGAAAAGGAAAAGCAGGATAAAGAGAAACAAGAAGAAAAGAAGGAGTCAGAAGTATCTAGAGAAGATGAATATAAGCAAAAGTATTCCAGAACATACGATGAGACTTATGCACGTTACAGACCAGTGTCAACTTCAAGTTCAAGCACTCCGTCGTCCTCCTCACTTTCTACTCTAGGCAGTTCACTCTATGCCTCAAGTCAGCTCAACAGGCCAAACAGCCTTGTAGGTATAACCTCTGCCTACTCCCGGGGATTAACCAAAGACAATGAAAGAGAGGGAGAGAAAAAAGAAGAGGAAAAAGAAGGGGAAGATAAGTCACAACCTAAATCAATCAGAGAACGACGGCGACCAAGAGAAAAACGGAGGTCTACTGGAGTCTCCTTCTGGACACAAGATAGTGATGAAAATGAGCAAGAGCGGCAGTCAGACACCGAGGATGGCTCCAGCAAGAGGGACACTCAGACGGATTCTGTTTCCAGGTATGACAGCAGTTCCACGTCATCAAGCGATCGGTATGACTCCTTGCTGGGTCGTTCTGCCTCATACAGTTACTTAGAAGAAAGGAAACCATATGGTAGCCGACTAGAAAAGGATGACTCAACTGACTTCAAAAAGCTTTATGAACAAATCTTAGCTGAAAATGAAAAACTAAAGGCACAGCTACATGACACAAATATGGAACTAACGGATCTAAAGTTGCAGTTGGAAAAAGCTACCCAGAGACAAGAACGATTTGCTGACAGGTCACTATTGGAGATGGAAAAAAGGGAACGAAGAGCTCTAGAAAGAAGAATATCTGAGATGGAAGAGGAGCTCAAAATGTTACCAGACTTAAAAGCAGACAACCAGAGGCTAAAGGATGAAAATGGGGCCTTGATCAGAGTTATAAGCAAACTTTCCAAGTAGGATCC

Amino Acid Sequence

MAHHHHHHMENLYFQGHMADGELNVDSLITRLLEVRGCRPGKIVQMTEAEVRGLCIKSREIFLSQPILLELEAPLKICGDIHGQYTDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRFNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDTGLLCDLLWSDPDKDVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPPRTANPPKKR

GST-MYPT1
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGAPGIPGATRAAAMKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLKLLHRGADINYANVDGLTALHQACIDDNVDMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGDTPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERIMLRDARQWLNSGHISDVRHAKSGGTALHVAAAKGYTEVLKLLIQAGYDVNIKDYDGWTPLHAAAHWGKEEACRILVDNLCDMETVNKVGQTAFDVADEDILGYLEELQKKQNLLHSEKRDKKSPLIESTANMENNQPQKTFKNKETLIIEPEKNASRIESLEQEKADEEEEGKKDESSCSSEEDEEDDSESEAETDKTKPMASVTNAHTASTQAAPAAVTTPTLSSNQGTPTSPVKKFPTSTTKISPKEEERKDESPASWRLGLRKTGSYGALAEITASKEAQKEKDTAGVIRSASSPRLSSSLDNKEKEKDNKGTRLAYVAPTIPRRLGSTSDIEEKENRESSNLRTSSSYTRRKWEDDLKKNSSINEGSTYHRSTSNRLWAEDSTEKEKDSAPTAATILVAPTVVSAAASSTTALTTTTAGTLSSTSEVRERRRSYLTPVRDEESESQRKARSRQARQSRRSTQGVTLTDLQEAEKTIGRSRSTRTREQENEEKDKEEKEKQDKEKQEEKKESEVSREDEYKQKYSRTYDETYARYRPVSTSSSSTPSSSSLSTLGSSLYASSQLNRPNSLVGITSAYSRGLTKDNEREGEKKEEEKEGEDKSQPKSIRERRRPREKRRSTGVSFWTQDSDENEQERQSDTEDGSSKRDTQTDSVSRYDSSSTSSSDRYDSLLGRSASYSYLEERKPYGSRLEKDDSTDFKKLYEQILAENEKLKAQLHDTNMELTDLKLQLEKATQRQERFADRSLLEMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU30200
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Species : ; PP1 beta BT019744(human AA), ; NM_001101722 (rabbit); MYPT1: NP_446342