cDNA Clone MYPT1 (rat)

• Plasmid: pJET-GST-MYPT1 (rat) w linker mut
• Parent Plasmid: pJet
• Species: Rat

Sequence of Insert

GGATCCAATAATTTTGTTTAACTTTAAGAAGGAGATATAAATATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTCGGAGCCCCAGGAATTCCCGGGGCGACTCGAGCGGCCGCGATGAAGATGGCGGACGCGAAGCAGAAGCGGAACGAGCAGCTGAAGCGCTGGATCGGCTCCGAGACGGACCTCGAGCCTCCCGTGGTGAAGCGCCAGAAGACCAAGGTGAAGTTCGACGATGGCGCCGTCTTCCTCGCCGCCTGCTCCAGCGGCGACACGGACGAGGTCCTCAAGCTGCTGCACCGCGGCGCCGACATCAATTACGCCAATGTGGACGGACTCACCGCCCTGCACCAGGCTTGCATTGATGACAATGTTGATATGGTGAAGTTTCTGGTAGAAAATGGAGCAAATATCAATCAACCTGACAATGAAGGCTGGATTCCACTCCATGCAGCCGCTTCCTGTGGATATCTGGATATTGCAGAATTTTTGATTGGTCAAGGAGCACATGTAGGAGCTGTCAACAGTGAAGGTGACACACCTTTAGATATTGCAGAGGAGGAAGCAATGGAAGAGCTACTTCAAAATGAGGTTAATCGGCAAGGTGTTGATATAGAAGCAGCTCGAAAAGAAGAGGAACGCATAATGCTTAGAGACGCGAGGCAGTGGTTGAACAGTGGTCACATCAGTGACGTCCGGCATGCAAAGTCCGGAGGCACAGCACTCCACGTGGCAGCGGCCAAAGGGTATACAGAAGTTTTAAAACTTTTAATACAGGCAGGCTATGATGTTAATATTAAAGATTATGATGGCTGGACACCTCTTCATGCTGCAGCTCACTGGGGTAAAGAAGAAGCATGTCGGATTTTAGTGGACAATCTGTGTGATATGGAGACGGTCAACAAAGTGGGCCAAACAGCCTTTGATGTAGCAGATGAAGACATTTTGGGATATCTAGAGGAGTTGCAAAAAAAACAAAATCTGCTCCATAGTGAAAAGCGGGATAAGAAATCTCCACTGATTGAATCAACAGCAAATATGGAAAATAATCAACCACAGAAGACTTTTAAAAACAAGGAAACGTTGATTATTGAGCCAGAGAAAAATGCATCTCGAATCGAGTCTCTGGAGCAAGAAAAGGCTGATGAGGAGGAGGAAGGCAAGAAGGATGAGTCCAGCTGCTCCAGTGAGGAGGATGAGGAGGATGACTCCGAGTCCGAAGCGGAGACAGATAAGACAAAACCCATGGCTTCTGTAACTAATGCTCACACTGCCAGCACTCAGGCAGCTCCTGCCGCTGTGACAACACCTACTCTGTCTTCCAACCAGGGGACCCCTACATCACCTGTTAAAAAGTTTCCTACATCAACTACAAAAATTTCTCCCAAAGAAGAAGAAAGAAAAGATGAATCTCCTGCATCCTGGAGGTTAGGACTTAGAAAGACTGGCAGTTATGGTGCCCTGGCTGAGATCACTGCATCTAAAGAAGCTCAGAAGGAGAAAGACACTGCAGGCGTGATACGTTCAGCTTCGAGTCCCAGACTCTCGTCCTCTTTGGATAATAAAGAAAAGGAGAAAGACAATAAAGGAACAAGACTTGCATATGTCGCCCCTACAATCCCAAGGCGACTAGGCAGTACGTCTGACATTGAAGAGAAGGAAAACAGAGAGTCTTCAAATTTGCGAACAAGTAGTTCTTACACAAGAAGAAAATGGGAAGATGATCTTAAAAAAAATAGTTCAATCAATGAAGGATCTACTTACCATAGAAGTACCTCAAATCGTTTGTGGGCTGAGGATAGTACTGAGAAAGAGAAGGACAGTGCTCCTACCGCAGCGACCATTCTTGTTGCTCCAACTGTTGTAAGTGCTGCAGCTTCTTCTACCACAGCCCTGACCACAACTACTGCTGGCACTCTTTCCTCCACATCAGAGGTCAGGGAGAGACGCAGGTCATACCTCACTCCTGTTAGGGATGAAGAGTCTGAATCCCAAAGGAAAGCAAGATCTAGACAAGCAAGACAGTCTAGACGGTCAACACAGGGGGTGACACTGACTGACCTCCAGGAAGCCGAAAAGACAATAGGAAGAAGCCGTTCTACGAGAACCAGAGAACAAGAAAACGAAGAAAAAGACAAAGAAGAAAAGGAAAAGCAGGATAAAGAGAAACAAGAAGAAAAGAAGGAGTCAGAAGTATCTAGAGAAGATGAATATAAGCAAAAGTATTCCAGAACATACGATGAGACTTATGCACGTTACAGACCAGTGTCAACTTCAAGTTCAAGCACTCCGTCGTCCTCCTCACTTTCTACTCTAGGCAGTTCACTCTATGCCTCAAGTCAGCTCAACAGGCCAAACAGCCTTGTAGGTATAACCTCTGCCTACTCCCGGGGATTAACCAAAGACAATGAAAGAGAGGGAGAGAAAAAAGAAGAGGAAAAAGAAGGGGAAGATAAGTCACAACCTAAATCAATCAGAGAACGACGGCGACCAAGAGAAAAACGGAGGTCTACTGGAGTCTCCTTCTGGACACAAGATAGTGATGAAAATGAGCAAGAGCGGCAGTCAGACACCGAGGATGGCTCCAGCAAGAGGGACACTCAGACGGATTCTGTTTCCAGGTATGACAGCAGTTCCACGTCATCAAGCGATCGGTATGACTCCTTGCTGGGTCGTTCTGCCTCATACAGTTACTTAGAAGAAAGGAAACCATATGGTAGCCGACTAGAAAAGGATGACTCAACTGACTTCAAAAAGCTTTATGAACAAATCTTAGCTGAAAATGAAAAACTAAAGGCACAGCTACATGACACAAATATGGAACTAACGGATCTAAAGTTGCAGTTGGAAAAAGCTACCCAGAGACAAGAACGATTTGCTGACAGGTCACTATTGGAGATGGAAAAAAGGGAACGAAGAGCTCTAGAAAGAAGAATATCTGAGATGGAAGAGGAGCTCAAAATGTTACCAGACTTAAAAGCAGACAACCAGAGGCTAAAGGATGAAAATGGGGCCTTGATCAGAGTTATAAGCAAACTTTCCAAGTAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGAPGIPGATRAAAMKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLKLLHRGADINYANVDGLTALHQACIDDNVDMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGDTPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERIMLRDARQWLNSGHISDVRHAKSGGTALHVAAAKGYTEVLKLLIQAGYDVNIKDYDGWTPLHAAAHWGKEEACRILVDNLCDMETVNKVGQTAFDVADEDILGYLEELQKKQNLLHSEKRDKKSPLIESTANMENNQPQKTFKNKETLIIEPEKNASRIESLEQEKADEEEEGKKDESSCSSEEDEEDDSESEAETDKTKPMASVTNAHTASTQAAPAAVTTPTLSSNQGTPTSPVKKFPTSTTKISPKEEERKDESPASWRLGLRKTGSYGALAEITASKEAQKEKDTAGVIRSASSPRLSSSLDNKEKEKDNKGTRLAYVAPTIPRRLGSTSDIEEKENRESSNLRTSSSYTRRKWEDDLKKNSSINEGSTYHRSTSNRLWAEDSTEKEKDSAPTAATILVAPTVVSAAASSTTALTTTTAGTLSSTSEVRERRRSYLTPVRDEESESQRKARSRQARQSRRSTQGVTLTDLQEAEKTIGRSRSTRTREQENEEKDKEEKEKQDKEKQEEKKESEVSREDEYKQKYSRTYDETYARYRPVSTSSSSTPSSSSLSTLGSSLYASSQLNRPNSLVGITSAYSRGLTKDNEREGEKKEEEKEGEDKSQPKSIRERRRPREKRRSTGVSFWTQDSDENEQERQSDTEDGSSKRDTQTDSVSRYDSSSTSSSDRYDSLLGRSASYSYLEERKPYGSRLEKDDSTDFKKLYEQILAENEKLKAQLHDTNMELTDLKLQLEKATQRQERFADRSLLEMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK*

• Vector Type: Other
• DU Number: DU30199
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Upstream of ATG 40 nt sequence is identical with good expressing POPH vectors