cDNA Clone MYPT1 (rat)

• Plasmid: pcDNA5-FRT/TO-FLAG MYPT1 wt
• Parent Plasmid: pcDNA5 FRT/TO FLAG2
• Expressed: FLAG-MYPT1 wt
• Genbank: NP_446342_
• Tag: FLAG
• Species: Rat

Sequence of Insert

GCGGCCGCGATGAAGATGGCGGACGCGAAGCAGAAGCGGAACGAGCAGCTGAAGCGCTGGATCGGCTCCGAGACGGACCTCGAGCCTCCCGTGGTGAAGCGCCAGAAGACCAAGGTGAAGTTCGACGATGGCGCCGTCTTCCTCGCCGCCTGCTCCAGCGGCGACACGGACGAGGTCCTCAAGCTGCTGCACCGCGGCGCCGACATCAATTACGCCAATGTGGACGGACTCACCGCCCTGCACCAGGCTTGCATTGATGACAATGTTGATATGGTGAAGTTTCTGGTAGAAAATGGAGCAAATATCAATCAACCTGACAATGAAGGCTGGATTCCACTCCATGCAGCCGCTTCCTGTGGATATCTGGATATTGCAGAATTTTTGATTGGTCAAGGAGCACATGTAGGAGCTGTCAACAGTGAAGGTGACACACCTTTAGATATTGCAGAGGAGGAAGCAATGGAAGAGCTACTTCAAAATGAGGTTAATCGGCAAGGTGTTGATATAGAAGCAGCTCGAAAAGAAGAGGAACGCATAATGCTTAGAGACGCGAGGCAGTGGTTGAACAGTGGTCACATCAGTGACGTCCGGCATGCAAAGTCCGGAGGCACAGCACTCCACGTGGCAGCGGCCAAAGGGTATACAGAAGTTTTAAAACTTTTAATACAGGCAGGCTATGATGTTAATATTAAAGATTATGATGGCTGGACACCTCTTCATGCTGCAGCTCACTGGGGTAAAGAAGAAGCATGTCGGATTTTAGTGGACAATCTGTGTGATATGGAGACGGTCAACAAAGTGGGCCAAACAGCCTTTGATGTAGCAGATGAAGACATTTTGGGATATCTAGAGGAGTTGCAAAAAAAACAAAATCTGCTCCATAGTGAAAAGCGGGATAAGAAATCTCCACTGATTGAATCAACAGCAAATATGGAAAATAATCAACCACAGAAGACTTTTAAAAACAAGGAAACGTTGATTATTGAGCCAGAGAAAAATGCATCTCGAATCGAGTCTCTGGAGCAAGAAAAGGCTGATGAGGAGGAGGAAGGCAAGAAGGATGAGTCCAGCTGCTCCAGTGAGGAGGATGAGGAGGATGACTCCGAGTCCGAAGCGGAGACAGATAAGACAAAACCCATGGCTTCTGTAACTAATGCTCACACTGCCAGCACTCAGGCAGCTCCTGCCGCTGTGACAACACCTACTCTGTCTTCCAACCAGGGGACCCCTACATCACCTGTTAAAAAGTTTCCTACATCAACTACAAAAATTTCTCCCAAAGAAGAAGAAAGAAAAGATGAATCTCCTGCATCCTGGAGGTTAGGACTTAGAAAGACTGGCAGTTATGGTGCCCTGGCTGAGATCACTGCATCTAAAGAAGCTCAGAAGGAGAAAGACACTGCAGGCGTGATACGTTCAGCTTCGAGTCCCAGACTCTCGTCCTCTTTGGATAATAAAGAAAAGGAGAAAGACAATAAAGGAACAAGACTTGCATATGTCGCCCCTACAATCCCAAGGCGACTAGGCAGTACGTCTGACATTGAAGAGAAGGAAAACAGAGAGTCTTCAAATTTGCGAACAAGTAGTTCTTACACAAGAAGAAAATGGGAAGATGATCTTAAAAAAAATAGTTCAATCAATGAAGGATCTACTTACCATAGAAGTACCTCAAATCGTTTGTGGGCTGAGGATAGTACTGAGAAAGAGAAGGACAGTGCTCCTACCGCAGCGACCATTCTTGTTGCTCCAACTGTTGTAAGTGCTGCAGCTTCTTCTACCACAGCCCTGACCACAACTACTGCTGGCACTCTTTCCTCCACATCAGAGGTCAGGGAGAGACGCAGGTCATACCTCACTCCTGTTAGGGATGAAGAGTCTGAATCCCAAAGGAAAGCAAGATCTAGACAAGCAAGACAGTCTAGACGGTCAACACAGGGGGTGACACTGACTGACCTCCAGGAAGCCGAAAAGACAATAGGAAGAAGCCGTTCTACGAGAACCAGAGAACAAGAAAACGAAGAAAAAGACAAAGAAGAAAAGGAAAAGCAGGATAAAGAGAAACAAGAAGAAAAGAAGGAGTCAGAAGTATCTAGAGAAGATGAATATAAGCAAAAGTATTCCAGAACATACGATGAGACTTATGCACGTTACAGACCAGTGTCAACTTCAAGTTCAAGCACTCCGTCGTCCTCCTCACTTTCTACTCTAGGCAGTTCACTCTATGCCTCAAGTCAGCTCAACAGGCCAAACAGCCTTGTAGGTATAACCTCTGCCTACTCCCGGGGATTAACCAAAGACAATGAAAGAGAGGGAGAGAAAAAAGAAGAGGAAAAAGAAGGGGAAGATAAGTCACAACCTAAATCAATCAGAGAACGACGGCGACCAAGAGAAAAACGGAGGTCTACTGGAGTCTCCTTCTGGACACAAGATAGTGATGAAAATGAGCAAGAGCGGCAGTCAGACACCGAGGATGGCTCCAGCAAGAGGGACACTCAGACGGATTCTGTTTCCAGGTATGACAGCAGTTCCACGTCATCAAGCGATCGGTATGACTCCTTGCTGGGTCGTTCTGCCTCATACAGTTACTTAGAAGAAAGGAAACCATATGGTAGCCGACTAGAAAAGGATGACTCAACTGACTTCAAAAAGCTTTATGAACAAATCTTAGCTGAAAATGAAAAACTAAAGGCACAGCTACATGACACAAATATGGAACTAACGGATCTAAAGTTGCAGTTGGAAAAAGCTACCCAGAGACAAGAACGATTTGCTGACAGGTCACTATTGGAGATGGAAAAAAGGGAACGAAGAGCTCTAGAAAGAAGAATATCTGAGATGGAAGAGGAGCTCAAAATGTTACCAGACTTAAAAGCAGACAACCAGAGGCTAAAGGATGAAAATGGGGCCTTGATCAGAGTTATAAGCAAACTTTCCAAATAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLKLLHRGADINYANVDGLTALHQACIDDNVDMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGDTPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERIMLRDARQWLNSGHISDVRHAKSGGTALHVAAAKGYTEVLKLLIQAGYDVNIKDYDGWTPLHAAAHWGKEEACRILVDNLCDMETVNKVGQTAFDVADEDILGYLEELQKKQNLLHSEKRDKKSPLIESTANMENNQPQKTFKNKETLIIEPEKNASRIESLEQEKADEEEEGKKDESSCSSEEDEEDDSESEAETDKTKPMASVTNAHTASTQAAPAAVTTPTLSSNQGTPTSPVKKFPTSTTKISPKEEERKDESPASWRLGLRKTGSYGALAEITASKEAQKEKDTAGVIRSASSPRLSSSLDNKEKEKDNKGTRLAYVAPTIPRRLGSTSDIEEKENRESSNLRTSSSYTRRKWEDDLKKNSSINEGSTYHRSTSNRLWAEDSTEKEKDSAPTAATILVAPTVVSAAASSTTALTTTTAGTLSSTSEVRERRRSYLTPVRDEESESQRKARSRQARQSRRSTQGVTLTDLQEAEKTIGRSRSTRTREQENEEKDKEEKEKQDKEKQEEKKESEVSREDEYKQKYSRTYDETYARYRPVSTSSSSTPSSSSLSTLGSSLYASSQLNRPNSLVGITSAYSRGLTKDNEREGEKKEEEKEGEDKSQPKSIRERRRPREKRRSTGVSFWTQDSDENEQERQSDTEDGSSKRDTQTDSVSRYDSSSTSSSDRYDSLLGRSASYSYLEERKPYGSRLEKDDSTDFKKLYEQILAENEKLKAQLHDTNMELTDLKLQLEKATQRQERFADRSLLEMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK

• Vector Type: Mammalian
• DU Number: DU30035
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee