cDNA Clone TTBK2

• Plasmid: pEGFP-C1 TTBK2 D163A
• Parent Plasmid: pEGFPC1
• Expressed: GFP TTBK2 D163A
• Genbank: AAH71556.1
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCACTAGTGGTACCATCGATGCGGCCGCGATGAGTGGGGGAGGAGAGCAGCCGGATATCCTGAGTGTTGG
AATCCTAGTGAAAGAAAGATGGAAAGTGTTGAGAAAGATTGGGGGTGGGG
GCTTTGGAGAAATTTACGATGCCTTGGACATGCTCACCAGGGAAAATGTT
GCACTGAAGGTGGAATCAGCTCAACAACCAAAACAAGTTCTGAAAATGGA
AGTTGCTGTTTTGAAAAAGCTGCAAGGGAAAGACCATGTTTGTAGATTTA
TTGGCTGTGGGAGGAATGATCGATTCAACTATGTGGTCATGCAGTTGCAG
GGTCGGAATCTGGCAGATCTTCGCCGTAGCCAGTCCCGAGGCACATTCAC
CATTAGTACCACTCTCCGGCTGGGTAGACAGATTTTGGAGTCTATTGAAA
GCATTCATTCTGTGGGATTCTTGCATCGAGACATCAAACCGTCGAACTTC
GCTATGGGTCGCTTTCCTAGTACATGTAGGAAATGTTACATGCTTGCTTT
TGGCTTGGCTCGACAATTTACCAATTCCTGTGGTGACGTCAGACCACCTC
GAGCTGTGGCAGGTTTTCGAGGGACAGTTCGTTATGCATCAATCAACGCA
CATCGGAACAGGGAAATGGGAAGACATGATGACCTTTGGTCCTTATTCTA
CATGTTGGTGGAGTTTGTGGTTGGTCAGCTGCCCTGGAGAAAAATAAAGG
ACAAGGAGCAAGTAGGCTCTATTAAGGAGAGATATGACCACAGGCTCATG
TTGAAACATCTCCCTCCAGAATTCAGCATCTTTCTAGACCATATCTCTTC
TTTGGATTATTTTACAAAACCAGACTACCAGCTTCTTACATCCGTGTTTG
ACAATAGCATCAAGACTTTTGGAGTAATTGAGAGTGACCCTTTTGACTGG
GAGAAGACTGGAAATGATGGCTCCCTAACAACCACCACTACTTCTACCAC
CCCTCAGTTGCACACTCGCTTGACCCCTGCTGCAATTGGAATTGCCAATG
CTACTCCCATCCCTGGAGACTTGCTTCGAGAAAATACAGATGAGGTATTT
CCAGATGAACAGCTTAGCGATGGAGAAAATGGCATCCCTGTTGGTGTGTC
ACCAGATAAATTGCCTGGATCTCTGGGACACCCCCGTCCCCAGGAGAAGG
ATGTTTGGGAAGAGATGGATGCCAACAAAAACAAGATAAAGCTTGGAATT
TGTAAGGCTGCTACTGAAGAGGAGAACAGCCATGGCCAGGCAAATGGTCT
TCTCAATGCTCCAAGCCTTGGGTCACCAATTCGTGTCCGCTCAGAGATTA
CTCAGCCAGACAGAGATATTCCACTGGTGCGAAAGTTACGTTCCATTCAC
AGCTTTGAGCTGGAAAAACGTCTGACCCTGGAGCCAAAGCCAGACACTGA
CAAGTTCCTTGAGACCTGCCTGGAGAAAATGCAGAAAGATACCAGTGCAG
GAAAAGAATCTATTCTCCCTGCTCTGCTGCATAAGCCTTGCGTTCCTGCT
GTGTCCCGTACTGACCACATCTGGCACTATGATGAAGAATATCTTCCAGA
TGCCTCCAAGCCTGCTTCTGCCAACACCCCTGAGCAGGCAGATGGTGGTG
GCAGCAATGGATTTATAGCTGTTAACCTGAGCTCTTGCAAGCAAGAAATT
GATTCCAAAGAATGGGTGATTGTGGACAAGGAGCAGGACCTTCAGGATTT
TAGGACAAATGAGGCTGTAGGACATAAAACAACTGGAAGTCCTTCTGATG
AGGAGCCTGAAGTACTTCAAGTCCTGGAGGCATCACCTCAAGATGAAAAG
CTCCAGTTAGGTCCTTGGGCAGAAAATGATCATTTAAAGAAGGAAACCTC
AGGTGTGGTCTTAGCACTTTCTGCAGAGGGTCCTCCTACTGCTGCTTCAG
AACAATATACAGATAGGCTGGAACTCCAGCCTGGAGCTGCTAGTCAGTTT
ATTGCAGCGACGCCCACAAGTCTAATGGAGGCGCAGGCAGAAGGACCCCT
TACAGCGATTACAATTCCTAGACCTTCTGTGGCATCTACACAGTCAACTT
CAGGAAGCTTTCACTGTGGTCAGCAGCCAGAGAAGAAAGATCTTCAGCCC
ATGGAGCCCACTGTGGAACTTTACTCTCCAAGGGAAAACTTCTCTGGCTT
GGTTGTGACAGAGGGTGAACCTCCTAGTGGAGGAAGCAGAACAGATTTGG
GGCTTCAGATAGATCACATTGGTCATGACATGTTACCCAACATTAGAGAA
AGTAACAAATCTCAAGACCTGGGACCAAAAGAACTTCCTGATCATAATAG
ACTGGTTGTGAGAGAATTTGAAAATCTCCCTGGGGAAACTGAAGAGAAAA
GCATCCTTTTAGAGTCAGATAATGAAGATGAGAAGTTAAGTAGAGGGCAG
CATTGTATTGAGATCTCCTCTCTCCCAGGAGATTTGGTAATTGTGGAAAA
GGATCACTCAGCTACTACTGAACCTCTTGATGTGACAAAAACACAGACTT
TTAGTGTGGTGCCAAATCAAGACAAAAATAATGAGATAATGAAGCTTCTG
ACAGTTGGAACTTCAGAAATTTCTTCCAGAGACATTGACCCACATGTTGA
AGGTCAGATAGGCCAAGTGGCAGAAATGCAAAAAAATAAGATATCTAAGG
ATGATGACATCATGAGTGAAGACTTGCCAGGTCATCAAGGAGACCTCTCT
ACTTTTTTGCACCAAGAGGGCAAGAGAGAGAAAATCACCCCTAGAAATGG
AGAACTATTTCATTGTGTTTCAGAGAATGAACATGGTGCCCCAACCCGGA
AGGATATGGTTAGGTCATCCTTTGTAACTAGACACAGCCGAATCCCTGTT
TTAGCACAAGAGATAGACTCAACTTTGGAATCATCCTCTCCAGTTTCTGC
AAAAGAAAAGCTCCTCCAAAAGAAAGCCTATCAGCCAGACCTAGTCAAGC
TTCTGGTGGAAAAAAGACAATTCAAGTCCTTCCTTGGCGACCTCTCAAGT
GCCTCTGATAAATTGCTAGAGGAGAAACTAGCTACTGTTCCTGCTCCCTT
TTGTGAGGAGGAAGTGCTCACTCCCTTTTCAAGACTGACAGTAGATTCTC
ACCTGAGTAGGTCAGCTGAAGATAGCTTTCTGTCACCCATCATCTCCCAG
TCTAGAAAGAGCAAAATTCCAAGGCCAGTTTCATGGGTCAACACAGATCA
GGTCAATAGCTCAACTTCGTCTCAGTTCTTTCCTCGGCCACCACCAGGAA
AGCCACCCACGAGGCCTGGAGTAGAAGCCAGGCTACGCAGATATAAAGTC
CTAGGGAGTAGTAACTCCGACTCAGACCTTTTCTCCCGCCTGGCCCAAAT
TCTTCAAAATGGATCTCAGAAACCCCGGAGCACTACTCAGTGCAAGAGTC
CAGGATCTCCTCACAATCCAAAAACACCACCCAAGAGTCCAGTTGTCCCT
CGCAGGAGTCCCAGTGCCTCTCCTCGAAGCTCATCCTTGCCTCGCACGTC
TAGTTCCTCACCATCTAGGGCTGGACGGCCCCACCATGACCAGAGGAGTT
CGTCCCCACATCTGGGGAGAAGCAAGTCACCTCCCAGCCACTCAGGATCT
TCCTCCTCCAGGAGGTCCTGCCAACAGGAGCATTGCAAACCCAGCAAGAA
TGGCCTGAAAGGATCCGGCAGCCTCCACCACCACTCAGCCAGCACTAAAA
CCCCCCAAGGGAAGAGTAAGCCAGCCAGTAAACTCAGCAGATAGCGGCCG
Caagggcgaattc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSTSGTIDAAAMSGGGEQPDILSVGILVKERWKVLRKIGGGGFGEIYDALDMLTRENV
ALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNDRFNYVVMQLQGRNLADLRRSQSRGTFTISTTLRLGRQILESIESIHSVGFLHRDIKPSNF
AMGRFPSTCRKCYMLAFGLARQFTNSCGDVRPPRAVAGFRGTVRYASINAHRNREMGRHDDLWSLFYMLVEFVVGQLPWRKIKDKEQVGSIKERYDHRLM
LKHLPPEFSIFLDHISSLDYFTKPDYQLLTSVFDNSIKTFGVIESDPFDWEKTGNDGSLTTTTTSTTPQLHTRLTPAAIGIANATPIPGDLLRENTDEVF
PDEQLSDGENGIPVGVSPDKLPGSLGHPRPQEKDVWEEMDANKNKIKLGICKAATEEENSHGQANGLLNAPSLGSPIRVRSEITQPDRDIPLVRKLRSIH
SFELEKRLTLEPKPDTDKFLETCLEKMQKDTSAGKESILPALLHKPCVPAVSRTDHIWHYDEEYLPDASKPASANTPEQADGGGSNGFIAVNLSSCKQEI
DSKEWVIVDKEQDLQDFRTNEAVGHKTTGSPSDEEPEVLQVLEASPQDEKLQLGPWAENDHLKKETSGVVLALSAEGPPTAASEQYTDRLELQPGAASQF
IAATPTSLMEAQAEGPLTAITIPRPSVASTQSTSGSFHCGQQPEKKDLQPMEPTVELYSPRENFSGLVVTEGEPPSGGSRTDLGLQIDHIGHDMLPNIRE
SNKSQDLGPKELPDHNRLVVREFENLPGETEEKSILLESDNEDEKLSRGQHCIEISSLPGDLVIVEKDHSATTEPLDVTKTQTFSVVPNQDKNNEIMKLL
TVGTSEISSRDIDPHVEGQIGQVAEMQKNKISKDDDIMSEDLPGHQGDLSTFLHQEGKREKITPRNGELFHCVSENEHGAPTRKDMVRSSFVTRHSRIPV
LAQEIDSTLESSSPVSAKEKLLQKKAYQPDLVKLLVEKRQFKSFLGDLSSASDKLLEEKLATVPAPFCEEEVLTPFSRLTVDSHLSRSAEDSFLSPIISQ
SRKSKIPRPVSWVNTDQVNSSTSSQFFPRPPPGKPPTRPGVEARLRRYKVLGSSNSDSDLFSRLAQILQNGSQKPRSTTQCKSPGSPHNPKTPPKSPVVP
RRSPSASPRSSSLPRTSSSSPSRAGRPHHDQRSSSPHLGRSKSPPSHSGSSSSRRSCQQEHCKPSKNGLKGSGSLHHHSASTKTPQGKSKPASKLSR*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU17482
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee