cDNA Clone
MYPT1 (rat)

Plasmid
pCMV-FLAG MYPT1 S445A S472A S856A
Parent Plasmid
pCMV5 FLAG2
Expressed
FLAG-MYPT1 S445A S472A S856A
Tag
FLAG
Species
Rat
Sequence of Insert
GCGGCCGCGATGAAGATGGCGGACGCGAAGCAGAAGCGGAACGAGCAGCTGAAGCGCTGGATCGGCTCCGAGACGGACCTCGAGCCTCCCGTGGTGAAGCGCCAGAAGACCAAGGTGAAGTTCGACGATGGCGCCGTCTTCCTCGCCGCCTGCTCCAGCGGCGACACGGACGAGGTCCTCAAGCTGCTGCACCGCGGCGCCGACATCAATTACGCCAATGTGGACGGACTCACCGCCCTGCACCAGGCTTGCATTGATGACAATGTTGATATGGTGAAGTTTCTGGTAGAAAATGGAGCAAATATCAATCAACCTGACAATGAAGGCTGGATTCCACTCCATGCAGCCGCTTCCTGTGGATATCTGGATATTGCAGAATTTTTGATTGGTCAAGGAGCACATGTAGGAGCTGTCAACAGTGAAGGTGACACACCTTTAGATATTGCAGAGGAGGAAGCAATGGAAGAGCTACTTCAAAATGAGGTTAATCGGCAAGGTGTTGATATAGAAGCAGCTCGAAAAGAAGAGGAACGCATAATGCTTAGAGACGCGAGGCAGTGGTTGAACAGTGGTCACATCAGTGACGTCCGGCATGCAAAGTCCGGAGGCACAGCACTCCACGTGGCAGCGGCCAAAGGGTATACAGAAGTTTTAAAACTTTTAATACAGGCAGGCTATGATGTTAATATTAAAGATTATGATGGCTGGACACCTCTTCATGCTGCAGCTCACTGGGGTAAAGAAGAAGCATGTCGGATTTTAGTGGACAATCTGTGTGATATGGAGACGGTCAACAAAGTGGGCCAAACAGCCTTTGATGTAGCAGATGAAGACATTTTGGGATATCTAGAGGAGTTGCAAAAAAAACAAAATCTGCTCCATAGTGAAAAGCGGGATAAGAAATCTCCACTGATTGAATCAACAGCAAATATGGAAAATAATCAACCACAGAAGACTTTTAAAAACAAGGAAACGTTGATTATTGAGCCAGAGAAAAATGCATCTCGAATCGAGTCTCTGGAGCAAGAAAAGGCTGATGAGGAGGAGGAAGGCAAGAAGGATGAGTCCAGCTGCTCCAGTGAGGAGGATGAGGAGGATGACTCCGAGTCCGAAGCGGAGACAGATAAGACAAAACCCATGGCTTCTGTAACTAATGCTCACACTGCCAGCACTCAGGCAGCTCCTGCCGCTGTGACAACACCTACTCTGTCTTCCAACCAGGGGACCCCTACATCACCTGTTAAAAAGTTTCCTACATCAACTACAAAAATTTCTCCCAAAGAAGAAGAAAGAAAAGATGAATCTCCTGCATCCTGGAGGTTAGGACTTAGAAAGACTGGCGCTTATGGTGCCCTGGCTGAGATCACTGCATCTAAAGAAGCTCAGAAGGAGAAAGACACTGCAGGCGTGATACGTTCAGCTGCGAGTCCCAGACTCTCGTCCTCTTTGGATAATAAAGAAAAGGAGAAAGACAATAAAGGAACAAGACTTGCATATGTCGCCCCTACAATCCCAAGGCGACTAGGCAGTACGTCTGACATTGAAGAGAAGGAAAACAGAGAGTCTTCAAATTTGCGAACAAGTAGTTCTTACACAAGAAGAAAATGGGAAGATGATCTTAAAAAAAATAGTTCAATCAATGAAGGATCTACTTACCATAGAAGTACCTCAAATCGTTTGTGGGCTGAGGATAGTACTGAGAAAGAGAAGGACAGTGCTCCTACCGCAGCGACCATTCTTGTTGCTCCAACTGTTGTAAGTGCTGCAGCTTCTTCTACCACAGCCCTGACCACAACTACTGCTGGCACTCTTTCCTCCACATCAGAGGTCAGGGAGAGACGCAGGTCATACCTCACTCCTGTTAGGGATGAAGAGTCTGAATCCCAAAGGAAAGCAAGATCTAGACAAGCAAGACAGTCTAGACGGTCAACACAGGGGGTGACACTGACTGACCTCCAGGAAGCCGAAAAGACAATAGGAAGAAGCCGTTCTACGAGAACCAGAGAACAAGAAAACGAAGAAAAAGACAAAGAAGAAAAGGAAAAGCAGGATAAAGAGAAACAAGAAGAAAAGAAGGAGTCAGAAGTATCTAGAGAAGATGAATATAAGCAAAAGTATTCCAGAACATATGATGAGACTTATGCACGTTACAGACCAGTGTCAACTTCAAGTTCAAGCACTCCGTCGTCCTCCTCACTTTCTACTCTAGGCAGTTCACTCTATGCCTCAAGTCAGCTCAACAGGCCAAACAGCCTTGTAGGTATAACCTCTGCCTACTCCCGGGGATTAACCAAAGACAATGAAAGAGAGGGAGAGAAAAAAGAAGAGGAAAAAGAAGGGGAAGATAAGTCACAACCTAAATCAATCAGAGAACGACGGCGACCAAGAGAAAAACGGAGGTCTACTGGAGTCTCCTTCTGGACACAAGATAGTGATGAAAATGAGCAAGAGCGGCAGTCAGACACCGAGGATGGCTCCAGCAAGAGGGACACTCAGACGGATTCTGTTTCCAGGTATGACAGCAGTTCCACGTCATCAAGCGATCGGTATGACTCCTTGCTGGGTCGTTCTGCCGCATACAGTTACTTAGAAGAAAGGAAACCATATGGTAGCCGACTAGAAAAGGATGACTCAACTGACTTCAAAAAGCTTTATGAACAAATCTTAGCTGAAAATGAAAAACTAAAGGCACAGCTACATGACACAAATATGGAACTAACGGATCTAAAGTTGCAGTTGGAAAAAGCTACCCAGAGACAAGAACGATTTGCTGACAGGTCACTATTGGAGATGGAAAAAAGGGAACGAAGAGCTCTAGAAAGAAGAATATCTGAGATGGAAGAGGAGCTCAAAATGTTACCAGACTTAAAAGCAGACAACCAGAGGCTAAAGGATGAAAATGGGGCCTTGATCAGAGTTATAAGCAAACTTTCCAAATAAGCGGCCGC
Amino Acid Sequence
MDYKDDDDKGIPGIPGSTRAAAMKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLKLLHRGADINYANVDGLTALH
QACIDDNVDMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGDTPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERIML
RDARQWLNSGHISDVRHAKSGGTALHVAAAKGYTEVLKLLIQAGYDVNIKDYDGWTPLHAAAHWGKEEACRILVDNLCDMETVNKVGQTAFDVADEDILG
YLEELQKKQNLLHSEKRDKKSPLIESTANMENNQPQKTFKNKETLIIEPEKNASRIESLEQEKADEEEEGKKDESSCSSEEDEEDDSESEAETDKTKPMA
SVTNAHTASTQAAPAAVTTPTLSSNQGTPTSPVKKFPTSTTKISPKEEERKDESPASWRLGLRKTGAYGALAEITASKEAQKEKDTAGVIRSAASPRLSS
SLDNKEKEKDNKGTRLAYVAPTIPRRLGSTSDIEEKENRESSNLRTSSSYTRRKWEDDLKKNSSINEGSTYHRSTSNRLWAEDSTEKEKDSAPTAATILV
APTVVSAAASSTTALTTTTAGTLSSTSEVRERRRSYLTPVRDEESESQRKARSRQARQSRRSTQGVTLTDLQEAEKTIGRSRSTRTREQENEEKDKEEKE
KQDKEKQEEKKESEVSREDEYKQKYSRTYDETYARYRPVSTSSSSTPSSSSLSTLGSSLYASSQLNRPNSLVGITSAYSRGLTKDNEREGEKKEEEKEGE
DKSQPKSIRERRRPREKRRSTGVSFWTQDSDENEQERQSDTEDGSSKRDTQTDSVSRYDSSSTSSSDRYDSLLGRSAAYSYLEERKPYGSRLEKDDSTDF
KKLYEQILAENEKLKAQLHDTNMELTDLKLQLEKATQRQERFADRSLLEMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK*
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU17428
Unit Source
Protein Phosphorylation and Ubiquitylation Unit
Location
PPU
MTA
Dundee
Price
£125.00
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