cDNA Clone SPAK (mouse)

• Plasmid: pEBG6P SPAK (mouse) 60-end
• Parent Plasmid: pEBG6P
• Expressed: GST-SPAK (mouse) 60-end
• Tag: GST
• Species: Mouse

Sequence of Insert

GCCCCGGCAGCTCAGGCGGTCGGCTGGCCCATCTGCAGGGACGCGTACGAGCTCCAGGAGGTTATCGGCAGCGGAGCAACTGCGGTGGTTCAGGCAGCCCTATGCAAACCCAGGCAAGAACGCGTAGCCATAAAGCGGATCAACTTGGAAAAATGCCAGACGAGTATGGATGAACTCCTGAAAGAAATTCAAGCCATGAGCCAATGCAGCCATCCCAACGTAGTGACTTATTACACCTCCTTTGTGGTCAAAGATGAGCTGTGGCTGGTCATGAAATTACTAAGTGGAGGTTCCATGTTGGATATCATCAAATACATCGTTAATCGAGGAGAACATAAAAATGGTGTCCTAGAAGAGGCGATAATTGCAACAATTCTTAAGGAGGTTTTGGAAGGTTTAGACTATCTGCACAGAAACGGTCAGATCCATAGGGATTTGAAAGCTGGAAATATTCTTCTGGGTGAGGATGGCTCAGTACAAATAGCAGATTTTGGAGTAAGCGCATTCTTAGCCACAGGGGGTGATGTTACACGGAATAAAGTCAGAAAAACATTTGTTGGTACCCCATGTTGGATGGCCCCTGAAGTCATGGAACAGGTGAGAGGCTATGACTTCAAGGCTGATATGTGGAGTTTTGGAATAACTGCCATTGAATTAGCAACCGGAGCAGCGCCTTACCACAAATACCCTCCAATGAAAGTGCTAATGCTGACTTTGCAAAATGACCCGCCCACTTTAGAAACTGGTGTAGAGGATAAAGAAATGATGAAAAAATACGGCAAGTCCTTCAGAAAGTTACTGTCACTGTGTCTTCAGAAAGATCCTTCCAAAAGGCCCACAGCAGCAGAACTTTTAAAATGCAAGTTCTTCCAGAAAGCCAAGAACAGAGAGTACCTGATCGAGAAGCTGCTGACAAGAACCCCAGACATAGCCCAAAGAGCCAAGAAGGTAAGGCGAGTTCCTGGGTCGAGCGGTCACCTTCATAAGACTGAAGACGGCGACTGGGAGTGGAGCGATGACGAGATGGATGAGAAGAGCGAGGAGGGGAAGGCAGCCGCCTCCCAAGAGAAGTCACGAAGAGTAAAAGAAGAGAACTCAGAGATCTCGGTGAATGCTGGTGGCATCCCTGAGCAAATACAGTCTCTCTCTGTGCACGACTCTCAGGCCCAACCAAACGCTAATGAAGACTACAGAGAAGGTCCTTGTGCAGTCAACCTTGTTTTAAGATTAAGAAACTCCAGAAAGGAACTTAATGACATACGATTTGAGTTTACTCCAGGAAGAGATACAGCAGACGGTGTGTCTCAGGAGCTCTTCTCTGCTGGCTTGGTTGACGGCCATGATGTAGTTATAGTGGCTGCTAATTTACAGAAGATTGTAGATGACCCCAAAGCTTTAAAAACGTTGACATTTAAGTTGGCTTCTGGCTGCGATGGGTCGGAGATTCCTGATGAAGTGAAGCTGATCGGGTTCGCCCAGTTGAGTGTGAGCTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSAPAAQAVGWPICRDAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAASQEKSRRVKEENSEISVNAGGIPEQIQSLSVHDSQAQPNANEDYREGPCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQLSVS*

• Vector Type: Mammalian
• DU Number: DU13527
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All SPAK plasmids MUST be grown at 30 degrees C or less to prevent recombination.