cDNA Clone EZH2

• Plasmid: pCMV5 EZH2-HA
• Parent Plasmid: pCMV5
• Expressed: EZH2-HA
• Genbank: NM_004456
• Tag: HA
• Species: Human

Sequence of Insert

GGTACCGCCACCATGGGCCAGACTGGGAAGAAATCTGAGAAGGGACCAGTTTGTTGGCGGAAGCGTGTAAAATCAGAGTACATGCGACTGAGACAGCTCAAGAGGTTCAGACGAGCTGATGAAGTAAAGAGTATGTTTAGTTCCAATCGTCAGAAAATTTTGGAAAGAACGGAAATCTTAAACCAAGAATGGAAACAGCGAAGGATACAGCCTGTGCACATCCTGACTTCTGTGAGCTCATTGCGCGGGACTAGGGAGTGTTCGGTGACCAGTGACTTGGATTTTCCAACACAAGTCATCCCATTAAAGACTCTGAATGCAGTTGCTTCAGTACCCATAATGTATTCTTGGTCTCCCCTACAGCAGAATTTTATGGTGGAAGATGAAACTGTTTTACATAACATTCCTTATATGGGAGATGAAGTTTTAGATCAGGATGGTACTTTCATTGAAGAACTAATAAAAAATTATGATGGGAAAGTACACGGGGATAGAGAATGTGGGTTTATAAATGATGAAATTTTTGTGGAGTTGGTGAATGCCCTTGGTCAATATAATGATGATGACGATGATGATGATGGAGACGATCCTGAAGAAAGAGAAGAAAAGCAG
AAAGATCTGGAGGATCACCGAGATGATAAAGAAAGCCGCCCACCTCGGAA
ATTTCCTTCTGATAAAATTTTTGAAGCCATTTCCTCAATGTTTCCAGATA
AGGGCACAGCAGAAGAACTAAAGGAAAAATATAAAGAACTCACCGAACAG
CAGCTCCCAGGCGCACTTCCTCCTGAATGTACCCCCAACATAGATGGACC
AAATGCTAAATCTGTTCAGAGAGAGCAAAGCTTACACTCCTTTCATACGC
TTTTCTGTAGGCGATGTTTTAAATATGACTGCTTCCTACATCGTAAGTGC
AATTATTCTTTTCATGCAACACCCAACACTTATAAGCGGAAGAACACAGA
AACAGCTCTAGACAACAAACCTTGTGGACCACAGTGTTACCAGCATTTGG
AGGGAGCAAAGGAGTTTGCTGCTGCTCTCACCGCTGAGCGGATAAAGACC
CCACCAAAACGTCCAGGAGGCCGCAGAAGAGGACGGCTTCCCAATAACAG
TAGCAGGCCCAGCACCCCCACCATTAATGTGCTGGAATCAAAGGATACAG
ACAGTGATAGGGAAGCAGGGACTGAAACGGGGGGAGAGAACAATGATAAA
GAAGAAGAAGAGAAGAAAGATGAAACTTCGAGCTCCTCTGAAGCAAATTC
TCGGTGTCAAACACCAATAAAGATGAAGCCAAATATTGAACCTCCTGAGA
ATGTGGAGTGGAGTGGTGCTGAAGCCTCAATGTTTAGAGTCCTCATTGGC
ACTTACTATGACAATTTCTGTGCCATTGCTAGGTTAATTGGGACCAAAAC
ATGTAGACAGGTGTATGAGTTTAGAGTCAAAGAATCTAGCATCATAGCTC
CAGCTCCCGCTGAGGATGTGGATACTCCTCCAAGGAAAAAGAAGAGGAAA
CACCGGTTGTGGGCTGCACACTGCAGAAAGATACAGCTGAAAAAGGACGG
CTCCTCTAACCATGTTTACAACTATCAACCCTGTGATCATCCACGGCAGC
CTTGTGACAGTTCGTGCCCTTGTGTGATAGCACAAAATTTTTGTGAAAAG
TTTTGTCAATGTAGTTCAGAGTGTCAAAACCGCTTTCCGGGATGCCGCTG
CAAAGCACAGTGCAACACCAAGCAGTGCCCGTGCTACCTGGCTGTCCGAG
AGTGTGACCCTGACCTCTGTCTTACTTGTGGAGCCGCTGACCATTGGGAC
AGTAAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAA
GCATCTATTGCTGGCACCATCTGACGTGGCAGGCTGGGGGATTTTTATCA
AAGATCCTGTGCAGAAAAATGAATTCATCTCAGAATACTGTGGAGAGATT
ATTTCTCAAGATGAAGCTGACAGAAGAGGGAAAGTGTATGATAAATACAT
GTGCAGCTTTCTGTTCAACTTGAACAATGATTTTGTGGTGGATGCAACCC
GCAAGGGTAACAAAATTCGTTTTGCAAATCATTCGGTAAATCCAAACTGC
TATGCAAAAGTTATGATGGTTAACGGTGATCACAGGATAGGTATTTTTGC
CAAGAGAGCCATCCAGACTGGCGAAGAGCTGTTTTTTGATTACAGATACA
GCCAGGCTGATGCCCTGAAGTATGTCGGCATCGAAAGAGAAATGGAAATC
CCTGGCGGTAGCTACCCATACGATGTGCCAGATTACGCCTGAGGATCC

Amino Acid Sequence

MGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKIL
ERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKT
LNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELI
KNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQ
KDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQ
QLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHRKC
NYSFHATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKT
PPKRPGGRRRGRLPNNSSRPSTPTINVLESKDTDSDREAGTETGGENNDK
EEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEASMFRVLIG
TYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRK
HRLWAAHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEK
FCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCGAADHWD
SKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEI
ISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNC
YAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEI
PGGSYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11197
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 87301 Daltons