cDNA Clone Puromycin

• Plasmid: pCMV5 Puro
• Parent Plasmid: pCMV5
• Genbank: P13249
• Species: Streptomyces alboniger

Sequence of Insert

gaattccttgccacaacccacaaggagacgaccttccatgaccgagtaca
agcccacggtgcgcctcgccacccgcgacgacgtcccccgggccgtacgc
accctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcga
cccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctca
cgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgcc
gcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgtt
cgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccg
cgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagccc
gcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaaggg
tctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccg
gggtgcccgccttcctggagacctccgcgccccgcaacctccccttctac
gagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggacc
gcgcacctggtgcatgacccgcaagcccggtgcctgacgcccgccccacg
acccgcagcgcccgaccgaaaggagcgcacgaccccatggctccgaccga
agccacccggggcggccccgccgaccccgcacccgcccccgaggcccacc
gactctagataactgatcataatcagccataccacatttgtagaggtttt
acttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaa
tgaatgcaattgttgttgttaacttgtttattgcagcttataatggttac
aaataaagcaatagcatcacaaatttcacaaataaagcatttttttcact
gcattctagttgtggtttgtccaaactcatcaatgtatcttaacgcgtcg
agtgcattctagttgtggAAGTTCCTATTCCGAAGTTCCTATTCttcaaa
taGTATAGGAACTTCatatggcgatcgcaattggccggccggatcc

Amino Acid Sequence

MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQ
ELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSG
SRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEA
AERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11114
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: This clone made to use as a template to amplify CMV-Puro for the generation of pBS CMV-Puro.Neo-IRES.TK plasmid for making targeting constructs.; ; Estimated MW of expressed protein : 21494 Daltons