cDNA Clone MLCK

• Plasmid: pGEX6P-1-smMLCK 475-838
• Parent Plasmid: pGEX6P1
• Expressed: GST-smMLCK 475-838
• Genbank: NM_005965
• Tag: GST
• Species: Human

Sequence of Insert

ggatccGACCACGAATATAAGTTCCGTGTACGTGCAATCAACGTGTATGG
AACCAGTGAGCCAAGCCAGGAGTCTGAACTCACAACGGTAGGAGAGAAAC
CTGAAGAGCCGAAGGATGAAGTGGAGGTGTCAGATGATGATGAGAAGGAG
CCCGAGGTTGATTACCGGACAGTGACAATCAATACTGAACAAAAAGTATC
TGACTTCTACGACATTGAGGAGAGATTAGGATCTGGGAAATTTGGACAGG
TCTTTCGACTTGTAGAAAAGAAAACTCGAAAAGTCTGGGCAGGGAAGTTC
TTCAAGGCATATTCAGCAAAAGAGAAAGAGAATATCCGGCAGGAGATTAG
CATCATGAACTGCCTCCACCACCCTAAGCTGGTCCAGTGTGTGGATGCCT
TTGAAGAAAAGGCCAACATCGTCATGGTCCTGGAGATCGTGTCAGGAGGG
GAGCTGTTTGAGCGCATCATTGACGAGGACTTTGAGCTGACGGAGCGTGA
GTGCATCAAGTACATGCGGCAGATCTCGGAGGGAGTGGAGTACATCCACA
AGCAGGGCATCGTGCACCTGGACCTCAAGCCGGAGAACATCATGTGTGTC
AACAAGACGGGCACCAGGATCAAGCTCATCGACTTTGGTCTGGCCAGGAG
GCTGGAGAATGCGGGGTCTCTGAAGGTCCTCTTTGGCACCCCAGAATTTG
TGGCTCCTGAAGTGATCAACTATGAGCCCATCGGCTACGCCACAGACATG
TGGAGCATCGGGGTCATCTGCTACATCCTAGTCAGTGGCCTTTCCCCCTT
CATGGGAGACAACGATAACGAAACCTTGGCCAACGTTACCTCAGCCACCT
GGGACTTCGACGACGAGGCATTCGATGAGATCTCCGACGATGCCAAGGAT
TTCATCAGCAATCTGCTGAAGAAAGATATGAAAAACCGCCTGGACTGCAC
GCAGTGCCTTCAGCATCCATGGCTAATGAAAGATACCAAGAACATGGAGG
CCAAGAAACTCTCCAAGGACCGGATGAAGAAGTACATGGCAAGAAGGAAA
TGGCAGAAAACGGGCAATGCTGTGAGAGCCATTGGAAGACTGTCCTCTTA
Ggaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSDHEYKFRVRAINVYGTSEPSQESELTTVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTINTEQKVSDFYDIEERLGSGKFGQVFRLVEKKTRKVWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1106
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; (Using complete ORF numbering: c1551t silent in D517, c2076t silent in N692)