cDNA Clone NHE1

• Plasmid: pGEX6P-1-NHE1 K510-end
• Parent Plasmid: pGEX6P1
• Expressed: GST-NHE1 K510-end
• Genbank: NM_003047
• Tag: GST
• Species: Human

Sequence of Insert

gaattcaaaaagcaagagacgaagcgctccatcaacgaagagatccacacacagttcctggaccaccttctgacaggcatcgaagacatctgtggccactacggtcaccaccactggaaggacaagctcaaccggtttaataagaaatatgtgaagaagtgtctgatagctggcgagcgctccaaggagccccagctcattgccttctaccacaagatggagatgaagcaggccatcgagctggtggagagcgggggcatgggcaagatcccctctgccgtctccaccgtctccatgcagaacatc
caccccaagtccctgccttccgagcgcatcctgccagcactgtccaagga
caaggaggaggagatccgcaaaatcctgaggaacaacttgcagaagacca
ggcagcggctgcggtcctacaacagacacacgctggtggcagacccctac
gaggaagcctggaaccagatgctgctccggaggcagaaggcccggcagct
ggagcagaagatcaacaactacctgacggtgccagcccacaagctggact
cacccaccatgtctcgggcccgcatcggctcagacccactggcctatgag
ccgaaggaggacctgcctgtcatcaccatcgacccggcttccccgcagtc
acccgagtctgtggacctggtgaatgaggagctgaagggcaaagtcttag
ggttgagccgggatcctgcaaaggtggctgaggaggacgaggacgacgat
gggggcatcatgatgcggagcaaggagacttcgtccccaggaaccgacga
tgtcttcacccccgcgcccagtgacagccccagctcccagaggatacagc
gctgcctcagtgacccaggcccacaccctgagcctggggagggagaaccg
ttcttccccaaggggcagtaagcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFKKQETKRSINEEIHTQ
FLDHLLTGIEDICGHYGHHHWKDKLNRFNKKYVKKCLIAGERSKEPQLIA
FYHKMEMKQAIELVESGGMGKIPSAVSTVSMQNIHPKSLPSERILPALSK
DKEEEIRKILRNNLQKTRQRLRSYNRHTLVADPYEEAWNQMLLRRQKARQ
LEQKINNYLTVPAHKLDSPTMSRARIGSDPLAYEPKEDLPVITIDPASPQ
SPESVDLVNEELKGKVLGLSRDPAKVAEEDEDDDGGIMMRSKETSSPGTD
DVFTPAPSDSPSSQRIQRCLSDPGPHPEPGEGEPFFPKGQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11059
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 61909 Daltons