cDNA Clone NHE1

• Plasmid: pCMV5-FLAG NHE1 K510-end
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-NHE1 K510-end
• Genbank: NM_003047
• Tag: FLAG
• Species: Human

Sequence of Insert

gaattcaaaaagcaagagacgaagcgctccatcaacgaagagatccacacacagttcctggaccaccttctgacaggcatcgaagacatctgtggccactacggtcaccaccactggaaggacaagctcaaccggtttaataagaaatatgtgaagaagtgtctgatagctggcgagcgctccaaggagccccagctcattgccttctaccacaagatggagatgaagcaggccatcgagctggtggagagcgggggcatgggcaagatcccctctgccgtctccaccgtctccatgcagaacatc
caccccaagtccctgccttccgagcgcatcctgccagcactgtccaagga
caaggaggaggagatccgcaaaatcctgaggaacaacttgcagaagacca
ggcagcggctgcggtcctacaacagacacacgctggtggcagacccctac
gaggaagcctggaaccagatgctgctccggaggcagaaggcccggcagct
ggagcagaagatcaacaactacctgacggtgccagcccacaagctggact
cacccaccatgtctcgggcccgcatcggctcagacccactggcctatgag
ccgaaggaggacctgcctgtcatcaccatcgacccggcttccccgcagtc
acccgagtctgtggacctggtgaatgaggagctgaagggcaaagtcttag
ggttgagccgggatcctgcaaaggtggctgaggaggacgaggacgacgat
gggggcatcatgatgcggagcaaggagacttcgtccccaggaaccgacga
tgtcttcacccccgcgcccagtgacagccccagctcccagaggatacagc
gctgcctcagtgacccaggcccacaccctgagcctggggagggagaaccg
ttcttccccaaggggcagtaagcggccgc

Amino Acid Sequence

MDYKDDDDKGSPEFKKQETKRSINEEIHTQFLDHLLTGIEDICGHYGHHH
WKDKLNRFNKKYVKKCLIAGERSKEPQLIAFYHKMEMKQAIELVESGGMG
KIPSAVSTVSMQNIHPKSLPSERILPALSKDKEEEIRKILRNNLQKTRQR
LRSYNRHTLVADPYEEAWNQMLLRRQKARQLEQKINNYLTVPAHKLDSPT
MSRARIGSDPLAYEPKEDLPVITIDPASPQSPESVDLVNEELKGKVLGLS
RDPAKVAEEDEDDDGGIMMRSKETSSPGTDDVFTPAPSDSPSSQRIQRCL
SDPGPHPEPGEGEPFFPKGQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11056
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 36356 Daltons