cDNA Clone NFkB p52

• Plasmid: pGEX6P-1-p52
• Parent Plasmid: pGEX6P1
• Expressed: GST-p52
• Genbank: X61498
• Tag: GST
• Species: Human

Sequence of Insert

gaattcGAGAGTTGCTACAACCCAGGTCTGGATGGTATTATTGAATATGA
TGATTTCAAATTGAACTCCTCCATTGTGGAACCCAAGGAGCCAGCCCCAG
AAACAGCTGATGGCCCCTACCTGGTGATCGTGGAACAGCCTAAGCAGAGA
GGCTTCCGATTTCGATATGGCTGTGAAGGCCCCTCCCATGGAGGACTGCC
CGGTGCCTCCAGTGAGAAGGGCCGAAAGACCTATCCCACTGTCAAGATCT
GTAACTACGAGGGACCAGCCAAGATCGAGGTGGACCTGGTAACACACAGT
GACCCACCTCGTGCTCATGCCCACAGTCTGGTGGGCAAGCAATGCTCGGA
GCTGGGGATCTGCGCCGTTTCTGTGGGGCCCAAGGACATGACTGCCCAAT
TTAACAACCTGGGTGTCCTGCATGTGACTAAGAAGAACATGATGGGGACT
ATGATACAAAAACTTCAGAGGCAGCGGCTCCGCTCTAGGCCCCAGGGCCT
TACGGAGGCCGAGCAGCGGGAGCTGGAGCAAGAGGCCAAAGAACTGAAGA
AGGTGATGGATCTGAGTATAGTGCGGCTGCGCTTCTCTGCCTTCCTTAGA
GCCAGTGATGGCTCCTTCTCCCTGCCCCTGAAGCCAGTCAtCTCCCAGCC
CATCCATGAcAGCAAATCTCCGGGGGCATCAAACCTGAAGATTTCTCGAA
TGGACAAGACAGCAGGCTCTGTGCGGGGTGGAGATGAAGTTTATCTGCTT
TGTGACAAGGTGCAGAAAGATGACATTGAGGTTCGGTTCTATGAGGATGA
TGAGAATGGATGGCAGGCCTTTGGGGACTTCTCTCCCACAGATGTGCATA
AACAGTATGCCATTGTGTTCCGGACACCCCCCTATCACAAGATGAAGATT
GAGCGGCCTGTAACAGTGTTTCTGCAACTGAAACGCAAGCGAGGAGGGGA
CGTGTCTGATTCCAAACAGTTCACCTATTACCCTCTGGTGGAAGACAAGG
AAGAGGTGCAGCGGAAGCGGAGGAAGGCCTTGCCCACCTTCTCCCAGCCC
TTCGGGGGTGGCTCCCACATGGGTGGAGGCTCTGGGGGTGCAGCCGGGGG
CTACGGAGGAGCTGGAGGAGGTGGCAGCCTCGGTTTCTTCCCCTCCTCCC
TGGCCTACAGCCCCTACCAGTCCGGCGCGGGCCCCATGgGcTGCTACCCG
GGAGGCGGGGGCGGGGCGTGAgtcgac

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFESCYNPGLDGIIEYDDFKLNSSIVEPKEPAPETADGPYLVIVEQPKQRGFRFRYGCEGPSHGGLPGASSEKGRKTYPTVKICNYEGPAKIEVDLVTHSDPPRAHAHSLVGKQCSELGICAVSVGPKDMTAQFNNLGVLHVTKKNMMGTMIQKLQRQRLRSRPQGLTEAEQRELEQEAKELKKVMDLSIVRLRFSAFLRASDGSFSLPLKPVISQPIHDSKSPGASNLKISRMDKTAGSVRGGDEVYLLCDKVQKDDIEVRFYEDDENGWQAFGDFSPTDVHKQYAIVFRTPPYHKMKIERPVTVFLQLKRKRGGDVSDSKQFTYYPLVEDKEEVQRKRRKALPTFSQPFGGGSHMGGGSGGAAGGYGGAGGGGSLGFFPSSLAYSPYQSGAGPMGCYPGGGGGA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1012
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion